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ProteomicsLEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation
Quantitative crosslinking analysis to probe in vitro conformational dynamics governing ESCRT-mediated nuclear envelope formation. CHMP7 was crosslinked with D12-BS3 while CHMP7 in the presence of LEM2 winged helix domain was crosslinked with H12-BS3. Samples were mixed after crosslinking and analy...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-24 | MSV000084837 | MassIVE
ProteomicsUnravelling hidden components of the chloroplast envelope proteome
Chloroplast is a major plant cell organelle that fulfills essential metabolic and biosynthetic functions. Located at the interface between the chloroplast and other cell compartments, the chloroplast envelope system is a strategic barrier controlling exchanges of ions, metabolites and proteins, thus...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-04-15 | PXD010545 | Pride
Lc-msms Chloroplast Arabidopsis Envelope 
TranscriptomicsNsaRS is a Cell-Envelope-Stress Sensing Two-Component System of Staphylococcus aureus
S. aureus possesses 16 two-component systems (TCS), two of which (GraRS & NsaRS) belong to the intramembrane-sensing histidine kinase (IM-HK) family, conserved within the firmicutes. NsaRS has recently been documented as being important for nisin-resistance in S. aureus. In this study we present a d...
ORGANISM(S): Staphylococcus aureus 
2011-05-16 | E-GEOD-27061 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of C2C12 cells cultured either in 20% serum (undifferentiated) or in 2% horse serum (differentiated) to detail the global changes of gene expression in lamins and nuclear envelope assoicated proteins during myogenesis
During muscle differentiation, myogenesis sepcific genes are differentially regulated, including Lamins that function at least in maintenance of nuclear architecture and regulation of gene expression. We used microarrays to detail the global changes of gene expression in lamins and nuclear envelope ...
ORGANISM(S): Mus musculus 
2007-11-22 | E-GEOD-4694 | biostudies-arrayexpress
ProteomicsIdentification of chloroplast envelope proteins with critical importance for cold acclimation
Differential proteome analysis allowed to identify envelope proteins critical for cold acclimation and frost tolerance in Arabidopsis thaliana.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-12-08 | PXD015794 | Pride
Ntt Shotgun proteomics Envelope Mex1 Chloroplast Proteomics 
ProteomicsComparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope
The nuclear envelope (NE) is a subdomain of the ER with a distinctive protein composition that underpins its role in nuclear compartmentalization. To expand an understanding of NE functions, we refined methods to reveal low abundance transmembrane (TM) proteins concentrated at the NE. De facto, th...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-01-25 | MSV000091154 | MassIVE
TranscriptomicsThe cell envelope stress response mediated by the LiaFSRLm three-component system of Listeria monocytogenes is controlled via the phosphatase activity of the bifunctional histidine kinase LiaSLm
Most members of the phylum Firmicutes harbor a two-component system, LiaSR, which is involved in the response to cell envelope stress elicited most notably by inhibitors of the lipid II cycle. In all LiaSR systems studied in detail LiaSR-mediated signal transduction was shown to be negatively contro...
ORGANISM(S): Listeria monocytogenes EGD-e 
2010-11-16 | E-GEOD-24107 | biostudies-arrayexpress
ProteomicsIdentification of HLA-B*57:01-restricted endogenous and HIV epitopes
A novel modified HLA-B*57:01-restricted epitope was identified in one of the reversed-phase fractions following immunoaffinity purification of HLA-peptide complexes from C1R cells expressing HIV envelope protein. Also includes six immunopeptidome datasets from several B57 and env transfected cell ly...
ORGANISM(S): HIV-1 M:B_HXB2R Homo sapiens (Human) 
2018-02-19 | PXD004471 | Pride
Hla-b*57:01 Hiv envelope Immunopeptidomics Kynurenine 
TranscriptomicsThe PDZ-binding motif of SARS-CoV envelope protein is a determinant of viral pathogenesis
A recombinant SARS-CoV lacking the envelope (E) protein is attenuated in vivo. Here we report that E protein PDZ-binding motif (PBM), a domain involved in protein-protein interactions, is a major virulence determinant in vivo. Elimination of SARS-CoV E protein PBM by using reverse genetics led to at...
ORGANISM(S): Mus musculus 
2014-10-31 | E-GEOD-52920 | biostudies-arrayexpress
ProteomicsCombining proximity labeling and crosslinking mass spectrometry for proteomic dissection of nuclear envelope interactome
Analysis of LAP1 interactome in HEK293T cells using proximity labeling or proximity labeling combined with crosslinking methods.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-20 | MSV000084606 | MassIVE
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