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ProteomicsNo One-Size-Fits-All: Method-Dependent Recovery of Distinct EV Subsets Across 11 Plasma derived EV Isolation Methods
We evaluated eleven EV isolation methods to define their performance charac-teristics and optimal applications. EVs were quantified by NanoFCM, profiled for tetraspanins (CD9, CD63, CD81) via MSD assays, and further characterized by LC-MS/MS proteomics.
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD075093 | Pride
Reproducibility Extracellular vesicles (evs) Isolation methods Standardization Liquid biopsy Proteomics Biomarkers 
ProteomicsIsolation of Extracellular Vesicles from human plasma samples: The importance of controls
EV isolation from the plasma using differential Ultracentrifugation method
ORGANISM(S): Homo sapiens (Human) 
2026-05-07 | PXD037903 | Pride
Ultracentrifugation Extracellular vesicles Plasma Isolation Thromboplastin 
ProteomicsRat EV proteins isolated from different tissues
Isolation and chrecterization of EV proteins in different organs.
ORGANISM(S): Rattus norvegicus (Rat) 
2022-02-15 | PXD020568 | Pride
Ev Rat 
ProteomicsCD9-HPLC-IAC Isolation of Serum Extracellular Vesicles for Proteomic Analysis
We developed a column-based CD9 antibody-immobilized HPLC immunoaffinity (CD9-HPLC-IAC) technology for EV isolation from a microliter-scale of serum for downstream proteomic analysis. The CD9-HPLC-IAC method achieved EV isolation from 40 μL of serum in 30 min with a yield of 8.0×109 EVs. Proteomic a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD027025 | Pride
Serum Immunoaffinity isolation Cd9 Extracellular vesicles Hplc Proteomics 
ProteomicsComparative Analysis of Extracellular Vesicle Isolation from Equine Serum and Plasma
To compare the efficiency, reproducibility, and proteomic profiles of extracellular vesicles (EVs) isolated from equine serum and plasma using size-exclusion chromatography (SEC) and differential ultracentrifugation (UC)+SEC, and to assess how the starting material influences EV cargo. Blood was col...
ORGANISM(S): Equus caballus (Horse) 
2026-01-23 | PXD068077 | Pride
Evs Exploris 480 Horse Sp3 Evosep one Sec uc 
UnknownRapid and efficient isolation platform for plasma extracellular vesicles: EV-FISHER.
Not available
S-EPMC9676503 | biostudies-literature
ProteomicsCharacterization of equine bronchoalveolar lavage fluid extracellular vesicle proteomes across four isolation methods
Growing evidence supports the importance of extracellular vesicle (EV) as mediators of communication in pathological processes, including those underlying respiratory disease. However, establishing methods for isolating and characterizing EVs remains challenging, particularly for respiratory samp...
ORGANISM(S): Equus caballus (Horse) 
2025-05-07 | PXD054702 | Pride
Methods optimization Respiratory Bronchoalveolar lavage Extracellular vesicles Equine Proteome Cell surface markers Lc-ms/ms 
UnknownEfficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer.
Not available
S-EPMC9099953 | biostudies-literature
ProteomicsIsolation defines identity: Functional consequences of EV purification strategies
The biological activity of extracellular vesicles (EVs) is largely defined by their molecular cargo, yet the impact of isolation workflows on EV proteomes and function remains incompletely understood. Here, we compared four isolation strategies for EVs derived from malignant ascites and ES-2 ovarian...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-10-23 | MSV000099567 | MassIVE
ProteomicsSecretome containing extracellular vesicles (EV) from cardiovascular progenitors (EV-CPC) vs the secretome containing EV from Fibroblasts (EV-FB)
Secretome containing extracellular vesicles (EV) seem to mediate the benefits of cell therapy for ischemic heart failure. Our project has the objective of comparing the secretome containing extracellular vesicles (EV) from cardiac progenitor cells (EV-CPC) vs the secretome containing EV from Fibrobl...
ORGANISM(S): Homo sapiens (Human) 
2023-09-22 | PXD022129 | Pride
Extracellular vesicles (ev) Fibroblasts Secretome from cardic progenitors Cardiovascular progenitor cells (cpc) 
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