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ProteomicsEvosep One Enables Robust Quantitative Deep Proteome Coverage using Tandem Mass Tags while Significantly Reducing Machine Time
The balance between comprehensively analyzing the proteome and using valuable mass spectrometry time is a genuine challenge in the field of proteomics. Multidimensional fractionation strategies have significantly increased proteome coverage, but often at the cost of increased mass analysis time, des...
ORGANISM(S): Homo sapiens (Human) 
2019-04-04 | PXD012520 | Pride
Evosep Nonsmall cell lung carcinoma (nsclc) patient-derived xenografts (pdx) Tmt Mass analysis time Lc-ms/ms 
ProteomicsFelipe Sheep EV DIA LC/MS/MS using the Orbitrap Astral
DIA analysis of sheep EVs using Evosep one and Orbitrap Astral
ORGANISM(S): Ovis aries 
2026-09-14 | PXD077206 | Pride
Ev Dia Astral Sheep 
ProteomicsOptimised Sample Handling Minimises Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics
Chemical proteomics approaches often yield low peptide recovery because they aim to enrich low-abundance proteins. Poor sample handling further reduces recovery through peptide adsorption to plastic surfaces and losses due to vacuum evaporation. We systematically mapped these losses across buffers, ...
ORGANISM(S): Homo sapiens (Human) 
2026-06-23 | PXD069322 | Pride
Evosep Optimisation Chemoproteomics 
ProteomicsOne-Tip enables comprehensive proteome coverage in minimal cells and single zygotes
We present One-Tip, a lossless proteomics methodology that seamlessly combines swift, one-pot sample preparation with narrow-window data-independent acquisition mass spectrometric analysis. With simplest sample processing, One-Tip reproducibly identifies > 9,000 proteins from ~1000 cells and ~ 6,000...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-09-15 | PXD044991 | Pride
Astral Proteomics Zygote 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - HEK293T - Global expression proteomics
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. This platform profiles cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiobiotin (IA-...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054105 | Pride
Data independant acquistion Label free quantification 
UnknownA Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-robust Proteomics.
Not available
S-EPMC6210218 | biostudies-literature
UnknownFully Automated Workflow for Integrated Sample Digestion and Evotip Loading Enabling High-Throughput Clinical Proteomics.
Not available
S-EPMC11251069 | biostudies-literature
ProteomicsAbundance analysis of chromatin-residing proteins during lineage conversion using protein aggregation capture (PAC)
We performed abundance analysis of chromatin-residing proteins in BLaER1 cells during the early stages of B-cell-to-macrophage transdifferentiation. We employed protein aggregation capture (PAC) to analyze chromatin-residing protein abundances at 0, 6 and 24 h after induction of transdifferentiation...
ORGANISM(S): Homo sapiens (Human) 
2026-05-27 | PXD076495 | Pride
Mass spectrometry Transdifferentiation Human Chromatin Blaer1 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - 80 compound screen (HEK293T and Jurkat)
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiob...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054127 | Pride
Chemoproteomics Data independant acquisition Label-free qauntification 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - Library Build, concentration response, structure activity relationships and in-cell treatment
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiob...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054145 | Pride
Chemoproteomics. lavel-free quantification Data independant acquisition 
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