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ProteomicsArabidopsis thaliana anti-all-WRKY pull-downs from whole seedlings
The experiments were performed to monitor dynamics in WRKY transcription factor abundancies upon treatment with flg22, a peptide derived from the bacterial flagella. Mock-treated or WT seedlings treated for 2 h with flg22 were used to prepare crude nuclear lysates. Then pull downs were performed wit...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-07-30 | PXD008971 | Pride
Wrky Arabidopsis Pull-down Flg22 
ProteomicsProteomic changes in Oryza sativa leaves in response to MSP1 and flg22 treatments
Rice blast disease caused by Magnaporthe oryzae is one of the most damaging diseases affecting rice productivity. Previously, we reported a novel M. oryzae- secreted protein MSP1, which triggers cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. To ...
ORGANISM(S): Oryza sativa (Rice) 
2018-07-06 | PXD008069 | Pride
Msp1 Label-free quantitative proteomics Magnaporthe oryzae Pamp-triggered immunity Rice 
ProteomicsBarley seed proteome analysis - Genes for seed longevity in barley identified by genomic analysis on near isogenic lines
Genes controlling differences in seed longevity between two barley (Hordeum vulgare) accessions were identified by combining a quantitative genetics approach with ˈomicsˈ technologies in Near Isogenic Lines (NILs). The NILs were derived from crosses between the spring barley landraces L94 from Ethio...
ORGANISM(S): Hordeum vulgare (Barley) 
2018-07-23 | PXD009464 | Pride
Longevity Seed Barley Label-free quantification 
ProteomicsLysine acetylation of chloroplast proteins
In photosynthesis, light is absorbed by the thylakoid embedded light harvesting pigment protein complexes (LHCs) that surround the photosynthetic reaction centers, photosystem II (PSII) and photosystem I (PSI). The distribution of light energy between the photosystems is balanced through state trans...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-07-03 | PXD007625 | Pride
Lysine Arabidopsis Acetylation Chloroplast 
ProteomicsOxidative stress-triggered interactions between the succinyl- and acetyl-proteomes of rice leaves
Protein lysine acylation, such as succinylation and acetylation are important posttranslational modification (PTM) mechanisms, with key roles in the regulation of diverse metabolic and cellular processes. Antibody-based affinity enrichment, high-resolution LC-MS/MS analysis and integrated bioinforma...
ORGANISM(S): Oryza sativa (Rice) 
2018-07-23 | PXD007175 | Pride
Lysine acetylation Hydrogen peroxide Proteome Lysine succinylation Rice 
ProteomicsTemporal proteome analyses of the alternative rubber crop Taraxacum koksaghyz
Taraxacum koksaghyz is currently one of the most investigated plants as a potential source of natural rubber. This poly(cis-1,4-isoprene) polymer and further valuable secondary metabolites are produced in laticifer cells, which exist as a network of elongated tubules throughout the plant and have a ...
ORGANISM(S): Taraxacum 
2020-05-28 | PXD011773 | Pride
Barnase Articulated laticifers Latex-deficient plant Taraxacum koksaghyz 
ProteomicsLysine acetylation of chloroplast proteins 2
In photosynthesis, light is absorbed by the thylakoid embedded light harvesting pigment protein complexes (LHCs) that surround the photosynthetic reaction centers, photosystem II (PSII) and photosystem I (PSI). The distribution of light energy between the photosystems is balanced through state trans...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-07-03 | PXD007630 | Pride
Arabidopsis Lc-msms Plant 
ProteomicsQuantitative analysis of the Arabidopsis thaliana leaf acetylome after treatment with deacetylase inhibitors trichostatin A and apicidin
Lysine acetylation is a common post-translational modification in eukaryotes and prokaryotes which is known to be involved in the regulation of various cellular processes, such as transcriptional activation, metabolic signaling, and energy homeostasis. Nevertheless, its role in plant primary metabol...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-10-25 | PXD006695 | Pride
Lysine acetylation Arabidopsis Photosynthesis Histone deacetylases Rubisco activase 
ProteomicsCysteine oxidation as a regulatory mechanism of Arabidopsis plastidial NAD-dependent malate dehydrogenase
Malate dehydrogenases (MDHs) catalyze a reversible NAD(P)-dependent-oxidoreductase reaction that plays an important role in central metabolism and redox homeostasis of plant cells. Recent studies suggest a moonlighting function of plastidial NAD-dependent MDH (plNAD-MDH) in plastid biogenesis, indep...
ORGANISM(S): Escherichia Coli 
2024-04-29 | PXD048384 | JPOST Repository
ProteomicsArabidopsis thaliana DOG1 pull-downs from seed tissues
Functionally complemented dog1-1 mutant with a YFP-DOG1 fusion protein under the control of its native promoter were used as starting material for in vivo pull-down of YFP: DOG1 and its interacting proteins from seed tissues in native conditions. DOG1 interacting proteins were identified by mass spe...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-07-07 | PXD006347 | Pride
Arabidopsis thaliana Dormancy Dog1 Seed Germination 
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