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ProteomicsK-562 GalNAc-T1/2 Bump-and-hole Whole cell lysate proteomics data
Engineered GalNAc-T glycosyltransferases were used to incorporate a chemically modified GalNAc analog into the glycoproteins on the cell surface of K-562 cells. The chemical modification included a bioorthogonal alkyne tag that allowed for introduction of a clickable, acid-cleavable biotin-picolyl-a...
ORGANISM(S): Homo sapiens (Human) 
2020-04-22 | PXD017989 | Pride
Human Mucin Bioorthogonal Galnac-t Click Glycoprotein K-562 
ProteomicsChemical Genetic Identification of Cyclin-G-associated kinase (GAK) Substrates Reveals its Role in Regulating Na+/K+-ATPase and Sipa1L1 in Neurons
Recent genetic evidence implicates the serine/threonine kinase cyclin G-associated kinase (GAK) as a Parkinson’s disease risk. However, its role in neuronal function and many downstream effectors remain unclear. Employing a chemical genetics method, we show here that the sodium potassium pump (Na+...
ORGANISM(S): Mus musculus (Mouse) 
2019-01-07 | PXD011319 | Pride
Cyclin-g-associated kinase Sodium potassium pump Atp1a3 Gak 
UnknownChemical genetic identification of GAK substrates reveals its role in regulating Na<sup>+</sup>/K<sup>+</sup>-ATPase.
Not available
S-EPMC6312924 | biostudies-literature
ProteomicsCell-type specific labelling of glycoproteins in engineered K-562 cells (BOCTAG)
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected cells incorpora...
ORGANISM(S): Homo sapiens (Human) 
2022-09-10 | PXD035430 | Pride
Human Mono-culture Silac Whole lysates K-562 
ProteomicsCell-type specific labelling of O-linked glycoproteins in engineered K-562 cells
We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected cells incorpora...
ORGANISM(S): Homo sapiens (Human) 
2022-09-10 | PXD035437 | Pride
Human Mono-culture Silac Whole lysates K-562 
ProteomicsMapping ISG15 conjugation sites associated with cytosolic lipid droplets in macrophages during infection with Mycobacterium tuberculosis
ISG15 is an interferon-stimulated genes (ISGs) and an ubiquitin-like protein that undergoes conjugation to substrate proteins. Similar to ubiquitin conjugation, ISG15 conjugation is mediated by the ISG15 conjugation machinery. Recognition of Mycobacterium tuberculosis (Mtb) induces the accumulation ...
ORGANISM(S): Mycobacterium tuberculosis H37Rv Homo sapiens (Human) 
2026-10-16 | PXD059415 | Pride
Macrophage Mycobacterium tuberculosis Bcg Isg15 Lipid droplet Mycobacterium bovis Bacteria Infection Ubiquitin Human 
ProteomicsMapping ISG15 conjugation sites in THP1 cells during infection with Mycobacterium tuberculosis
ISG15 is an interferon-stimulated genes (ISGs) and an ubiquitin-like protein that undergoes conjugation to substrate proteins. Similar to ubiquitin conjugation, ISG15 conjugation is mediated by the ISG15 conjugation machinery. Recognition of Mycobacterium tuberculosis (Mtb) induces the accumulation ...
ORGANISM(S): Mycobacterium tuberculosis H37Rv Homo sapiens (Human) 
2026-10-16 | PXD075338 | Pride
Human Mycobacterium tuberculosis Isg15 Lipid droplets 
UnknownHIV-1 Infection of Primary CD4+ T Cells Regulates the Expression of Specific Human Endogenous Retrovirus HERV-K (HML-2) Elements.
Not available
S-EPMC5730760 | biostudies-literature
UnknownDegradation by Design: New Cyclin K Degraders from Old CDK Inhibitors.
Not available
S-EPMC10804372 | biostudies-literature
UnknownARK: Aggregation of Reads by K-Means for Estimation of Bacterial Community Composition.
Not available
S-EPMC4619776 | biostudies-literature
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