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We performed the cleavage under targets and tagmentation (Cut & tag) assay followed by sequencing enriched DNA fragments to reveal the direct downstream targets of Pbx1. Firstly, we overexpressed Pbx1b with Pbx1b-IRES-GFP retrovirus in murine peripheral B cells to ensure the yields of DNA fragments....
ORGANISM(S): Mus musculus 
The MAM326 cancer cell line was derived from a mammary tumor of bitransgenic FVB/N s-SHIP-GFP X C(3)1-Tag mice. The MAM326 cell line contained approximatively 10% of s-SHIP/GFP+ cells. This cell subpopulation was more resistant to drugs. To investigate the molecular basis of this chemoresistance, GF...
ORGANISM(S): Mus musculus 
Identify CTPS1 partners obtained by immunoprecipitation of a GFP-CTPS1 fusion protein (N-terminal tag) expressed in the HEK 293T cell line.
ORGANISM(S): Homo sapiens (Human) 
2025-09-10 | PXD047654 | Pride
Genome-wide target genes of ZmAN3 were identified through ChIP-seq on the growth zone of the maize leaf, encompassing the division, transition and expansion zone. For ChIP-seq, ZmAN3 was fused to the GSyellow TAP tag and expressed from the ubiquitin promoter (pUBIL). The pUBIL:ZmAN3-GSyellow constru...
ORGANISM(S): Zea mays 
Tiling ChIP-CHIP Ssn6-GFP, Tp11-GFP, Tup12-GFP and Hst4 vs WT Expression 6 Samples
ORGANISM(S): Schizosaccharomyces pombe 
Genomics
The CUT&Tag of ZmWRKY38-GFP
Single cells from Ptenpc-/-Smad4pc-/-mTmG+ prostate tumors were isolated into single cells which were FACS-sorted for GFP+ and Tomato+ cells and RNA was purified with TRIzol (Life Technologies). RNA expression profiling was performed using the Mouse Genome 430 2.0 Array (Affymetrix) to generate a P...
ORGANISM(S): Mus musculus 
In order to identify epigenetic protein complexes recruited to histone PTMs in the deadly human malaria parasite Plasmodium falciparum, we set out to perform histone peptide pulldowns with various histone peptides. Biotinylated peptides corresponding to P. falciparum histone variant H2A.Z, H2B.Z and...
ORGANISM(S): Plasmodium falciparum 
2019-10-15 | PXD010644 | Pride
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