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ProteomicsIP-MS of GFP tagged MRV μ2 protein
To determine which cellular proteins interacts with the viral MRV protein μ2 and its mutant μ2-P208S, GFP-tagged constructions of μ2 and μ2-P208S in both C- and N- terminus were transfected in 293T cells alongside control GFP alone for 24h, and submitted to GFP pulldown before IP were prepared for M...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD029701 | Pride
Virus ip-ms ap-ms gfp mrv reovirus μ2 
ProteomicsProteomics data of GFP::PGL-3 IP-MS compared to GFP control
We carried out immunoprecipitation-mass spectrometry (IP-MS) analysis to identify the protein components of PGL granules in heat-stressed embryos. GFP::PGL-3 proteins were immunoprecipitated from extracts of embryos grown at 26 oC and the interacting proteins were identified by LC-MS/MS. Proteins in...
ORGANISM(S): Caenorhabditis elegans 
2023-04-01 | PXD040246 | Pride
Gfp::pgl-3 Ip-ms C.elegans Pgl granules 
ProteomicsPlasmodium falciparum bromo-domain containing epigenetic protein complexes recruited to modified histone tails as identified by histone peptide pulldown (HPP)-MS/MS and further characterized by GFP-IP-MS/MS and HA-IP-MS/MS
In order to identify epigenetic protein complexes recruited to histone PTMs in the deadly human malaria parasite Plasmodium falciparum, we set out to perform histone peptide pulldowns with various histone peptides. Biotinylated peptides corresponding to P. falciparum histone variant H2A.Z, H2B.Z and...
ORGANISM(S): Plasmodium falciparum 
2019-10-15 | PXD010644 | Pride
Epigenetic complex Bromo-domain protein Hpp Gcn5 Gfp-ip Histone peptide pulldown Interactor Plasmodium falciparum Taf1 Histone acetylation Ha-ip Reader complex Histone methylation 
TranscriptomicsAIN-2::GFP IP of mRNAs associated with miRISC in stage sychronized C. elegans populations
AIN-2::GFP IP were used to purify miRISC from stage sychronized C.elegans populations (Egg, L1, L2, L3 and L4 stages). The mRNA composition of the IP results and the corresponding total RNA samples were analyzed by WUSTL Caenorhabditis elegans Whole Genome 23k Oligo Array. The miRISC associated mRNA...
ORGANISM(S): Caenorhabditis elegans 
2009-05-21 | E-GEOD-16208 | biostudies-arrayexpress
ProteomicsRNA Pol II IP-MS and GFP-CUL3 IP-MS by DIA
DIA MS dataset for RNA Polymerase II (Pol II) or GFP-CUL3 co-immunoprecipitants prepared from human DLD-1 SPT5-AID cells treated with +/- NVP-2 +/- auxin (for Pol II IP) or +/- auxin (GFP IP).
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-11-26 | MSV000096543 | MassIVE
ProteomicsCharacterization of GFP-Gdown1 interactors by IP and LC-MS/MS
Approximately half of purified mammalian RNA polymerase II (Pol II) is associated with a tightly interacting sub-stoichiometric subunit, Gdown1. Previous studies have established that Gdown1 inhibits transcription initiation through competitive interactions with general transcription factors and blo...
ORGANISM(S): Homo sapiens (Human) 
2022-02-14 | PXD029425 | Pride
Gfp Human Ip Gdown1 
OtherAIN-2::GFP IP of mRNAs associated with miRISC in stage sychronized C. elegans populations
AIN-2::GFP IP were used to purify miRISC from stage sychronized C.elegans populations (Egg, L1, L2, L3 and L4 stages). The mRNA composition of the IP results and the corresponding total RNA samples were analyzed by WUSTL Caenorhabditis elegans Whole Genome 23k Oligo Array. The miRISC associated mRNA...
ORGANISM(S): Caenorhabditis elegans 
2009-05-22 | GSE16208 | GEO
ProteomicsProtein interaction partners of FRUITFULL in inflorescence meristems and pistils by IP-MS.
To study the possibility that the tissue-specific gene targets and regulation by FUL are due to a different composition in the FUL transcription factor (TF) protein complex/es in both tissues, we determined the protein-protein interactions of FUL in planta. Inflorescence meristems (IMs) of 35S:AP1-G...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-03-30 | PXD037091 | Pride
Arabidopsis thaliana Ip-ms Protein complex Fruitfull 
ProteomicsGFP-Trap-IP-MS
To analyze proteins copurified with GFP-ALLO-1, worm eggs expressing GFP-ALLO-1 or GFP in the atg-11 background were lysed in co-IP buffer, and GFP-ALLO-1 or GFP was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were...
ORGANISM(S): Caenorhabditis Elegans 
2018-01-31 | PXD007847 | JPOST Repository
TranscriptomicsIdentification of the miRNA targetome in hippocampal neurons using RIP-seq
We established a neuron-specific Argonaute2:GFP-RNA immunoprecipitation followed by high throughput sequencing (AGO2-RIP-seq) to analyse the regulatory role of miRNAs in mouse hippocampal neurons. Using this technique, we identified more than two thousand miRNA target genes in hippocampal neurons, r...
ORGANISM(S): Mus musculus 
2015-07-31 | E-GEOD-65543 | biostudies-arrayexpress
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