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ProteomicsPlasmodium falciparum bromo-domain containing epigenetic protein complexes recruited to modified histone tails as identified by histone peptide pulldown (HPP)-MS/MS and further characterized by GFP-IP-MS/MS and HA-IP-MS/MS
In order to identify epigenetic protein complexes recruited to histone PTMs in the deadly human malaria parasite Plasmodium falciparum, we set out to perform histone peptide pulldowns with various histone peptides. Biotinylated peptides corresponding to P. falciparum histone variant H2A.Z, H2B.Z and...
ORGANISM(S): Plasmodium falciparum 
2019-10-15 | PXD010644 | Pride
Epigenetic complex Bromo-domain protein Hpp Gcn5 Gfp-ip Histone peptide pulldown Interactor Plasmodium falciparum Taf1 Histone acetylation Ha-ip Reader complex Histone methylation 
MetabolomicsGolgi-IP, a tool for multimodal analysis of Golgi molecular content
The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emerged as a docking platform for cellular signaling pathways inc...
2023-06-27 | MTBLS6511 | MetaboLights
ProteomicsBVES-HA IP-mass from AAV9-BVES-HA injected mouse skeletal muscle
To identify BVES-interacting proteins from mouse skeletal muscle, we performed IP-mass from AAV9-BVES-HA injected mouse skeletal muscle using the anti-HA magnetic beads.
ORGANISM(S): Mus musculus (Mouse) 
2023-02-27 | PXD036346 | Pride
Bves Ap-ms Mouse Skeletal muscle 
ProteomicsWhole-cell abundance profiling and interaction analysis of UBQLN2
To address whether expression of ALS/FTD-associated UBQLN2 mutant variants changes the cellular proteome, we performed label-free quantification-based global protein abundance profiling of patient-derived LCLs and CRISPR engineered HeLa cells. UBQLN2 mutant in both cell lines carried the mutation T4...
ORGANISM(S): Homo sapiens (Human) 
2022-06-24 | PXD029747 | Pride
Whole proteome Ha-ip Hela Lcl Als/ftd Ubqln2 
TranscriptomicsMicroRNA-Directed siRNA Biogenesis in Caenorhabditis elegans
C.elegans small RNAs from HA::ALG-1, HA::ALG-2 and HA::RDE-1 IP and rde-1 mutants Small RNAs were cloned from transgenic or mutant C. elegans adults. Sequencing was performed using 454 and Illumina platforms.
ORGANISM(S): Caenorhabditis elegans 
2010-04-14 | E-GEOD-20649 | biostudies-arrayexpress
TranscriptomicsProfiling of Argonaute (AGO) 2-associated sRNAs after infection of bacterial pathogen Pseudomonas syringae
Transgenic Arabidopsis plants (AGO2::HA:AGO2) were treated with either mock (10 mM MgCl2) or Pseudomonas syringae pv. tomato (Pst) expressing avrRpt2 (R2) at a concentration of 2 x 107 cfu/ml for 14 hours. sRNAs associated with AGO1 and AGO2 were co-immunoprecipitated using antibodies against either...
ORGANISM(S): Arabidopsis thaliana 
2011-05-10 | E-GEOD-26161 | biostudies-arrayexpress
ProteomicsSIK2-dependent Phosphoproteome and Interactome upon Salmonella infection
HeLa cells transfected with non-targeting control- and anti-SIK2 siRNA were left uninfected or infected with Salmonella and lysed 30 min pi. Following tryptic-digest peptides were labeled using TMT-pro 16-plex and phosphopeptides enriched. The endogenously tagged SIK2-HA IP workflow. Hela wild type ...
ORGANISM(S): Homo sapiens (Human) 
2021-05-06 | PXD021859 | Pride
Homo sapiens (human) 
ProteomicsIdentification of co-factors of eIF4E1b protein in mouse ovary.
We have identified eukaryotic translation initiation factor 4E family member 1B (Eif4e1b) as a regulator of maternal RNA translation, whose maternal deletion leads to 2-cell arrest phenotype in mouse embryos. eIF4E1b protein binds mRNA selectively in oocytes and controls their translation in early e...
ORGANISM(S): Mus musculus (Mouse) 
2023-06-12 | PXD041622 | Pride
Mouse Initiation facto Eif-4e1b Affinity Ovary 
ProteomicsC-terminal amides mark proteins for degradation via SCF/FBXO31 - Interaction Proteomics of FBXO31 in HEK293T cells
FBXO31 is a substrate adapter for the SCF-type ubiquitin ligase complex SCF/FBXO31 which ubiquitylates C-terminal amide-bearing proteins (CTAPs), thereby triggering their proteasomal degradation in human cells. Immunoprecipitation followed by MS (IP-MS) was performed on HA-tagged cDNA-expressed var...
ORGANISM(S): Homo sapiens (Human) 
2024-11-05 | PXD055535 | Pride
Ap-ms Fbxo31 Ip-ms Oxidative stress Ubiquitin ligase 
ProteomicsLC-MS of interacting partners Toxoplasma gondii GRA57
We identified GRA57 as a Toxoplasma gondii secreted effector required for parasite survival of interferon gamma in HFFs. To identify interaction partners of GRA57 during infection of HFFs, which could indicate how GRA57 functions to protect the parasite, we performed co-immunoprecipitation (IP) expe...
ORGANISM(S): Homo sapiens (Human) Toxoplasma gondii RH 
2023-06-21 | PXD041352 | Pride
Toxoplasma gondii Hff Effectors Dense granule 
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