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ProteomicsLarge-scale filter-aided sample preparation method for the analysis of the ubiquitinome
Protein ubiquitination regulates key cellular functions including protein homeostasis and signal transduction. The digestion of ubiquitinated proteins with trypsin yields a glycine-glycine remnant bound to the modified lysine residue (K-ε-GG) that can be recognized by specific antibodies for immunoa...
ORGANISM(S): Homo sapiens (Human) 
2017-03-30 | PXD005421 | Pride
Ubiquitinome Large-scale Portein digestion Post-translational modifications Lfasp Proteome 
ProteomicsThe ubiquitin landscape of the Influenza A Virus polymerase
During influenza A virus (IAV) infections, viral proteins are targeted by cellular E3 ligases for modification with ubiquitin. Here, we decipher and functionally explore the ubiquitin landscape of the IAV polymerase during infection of human alveolar epithelial cells by applying mass spectrometry an...
ORGANISM(S): Homo sapiens (Human) Influenza A virus (A/Wilson-Smith/1933(H1N1)) 
2023-03-11 | PXD030816 | Pride
Influenza a virus Pb1 Affinity enrichment Pa Viral rna-dependent rna polymerase Ubiquitination K-ε-gg motif Lc-msms Pb2 
ProteomicsUbiquitin-mediated modulation of necroptotic effector MLKL
We aimed to identify lysine (K) residues that are endogenously ubiquitylated upon TNF-induced necroptosis. To this end, we used a quantitative mass spectrometry-based approach. Briefly, MDFs were treated with TSZ or left untreated and protein lysates were digested with trypsin into peptides. Ubiquit...
ORGANISM(S): Mus musculus (Mouse) 
2021-04-13 | PXD018857 | Pride
Ubiquitin Tmt Cancer Mlkl Mouse Msms 
ProteomicsMapping Ubiquitination Sites in Alzheimer's Disease
Alzheimer’s Disease (AD) is characterized by the deposition of protein aggregates such as neurofibrillary tangles (NFTs) and amyloid (Aβ) plaques in the brain. Post-translational modification through ubiquitylationplays critical roles in clearing misfolded protein aggregates. Investig...
ORGANISM(S): Homo sapiens (Human) 
2019-06-25 | PXD009199 | Pride
Ubiquitylome Ad Gg remnant peptides 
ProteomicsRefined preparation and use of K-GG antibody enables routine quantification of ubiquitination sites
Not available
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-04-03 | MSV000079097 | MassIVE
ProteomicsMus musculus primary CD4+ T cell di-glycine remnant and whole cell proteomics
It is known that ubiquitination is important for T cell receptor (TCR) signaling during T cell activation but the breadth of ubiquitination events triggered during TCR signaling is not completely understood. This dataset utilizes di-glycine remnant profiling combined with mass spectrometry to identi...
ORGANISM(S): Mus musculus (Mouse) 
2019-05-09 | PXD012831 | Pride
Mus musculus primary cd4+ t cell di-glycine remnant and whole cell proteomics 
ProteomicsUbiquitin remnant profiling for identification of the ubiquitylation sites in mysterin
Ubiquitin remnants obtained from the cells expressing variants of ubiquitin ligase mysterin were analyzed by mass spectrometry to identify the ubiquitylation sites in mysterin. HN indicates the RING finger mutant (H4014N). RZ indicates the RZ finger mutant (H4509A). A1A2 indicates the ATPase mutant ...
ORGANISM(S): Homo sapiens (Human) 
2026-10-02 | PXD055333 | Pride
Lc-msms Ubiquitinome Human cells 
ProteomicsProteome wide screening for ubiquitination, ISGylation and NEDDylation sites in wildtype and ISG15 knockout mouse hearts after transverse aortic constriction
Hearts(left ventricles) were harvested from control wildtype and ISG15 knockout mice and wildtype and ISG15 knockout mice 4 weeks after pressure overload induced by transverse aortic constriction. Nano LC-MS/MS analysis was performedon left ventricle tissue following protein extraction, trypsin dige...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-10 | PXD032267 | Pride
Isgylation Mouse K-e-gg motif Heart Ubiquitination Tac 
ProteomicsTCellXTalk enables the detection of co-modified peptides for the study of protein post-translational modification cross-talk in T cells
Protein function is regulated by post-translational modifications (PTMs) that may act individually or interact with others in a phenomenon termed PTM cross-talk. Multiple databases have been dedicated to PTMs, including recent initiatives oriented to the in silico prediction of PTM interactions. The...
ORGANISM(S): Homo sapiens (Human) 
2018-09-19 | PXD010176 | Pride
Ptms Cross-talk Tcr activation T cells 
ProteomicsQuantitative K-GG site analysis
Post-translational modification of proteins by ubiquitin (UQ) and UQ-like modifiers is emerging as a central pathway in DNA replication. We previously showed that chromatin in the vicinity of replisomes is rich in SUMO and depleted in UQ, whereas an opposite pattern is observed in mature chromatin. ...
ORGANISM(S): Homo sapiens (Human) 
2016-01-13 | PXD002408 | Pride
Ubiquitylation Usp7 inhibitor Lc-ms/ms 
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