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H157 cells transfected with vector and overexpressed AACT plasmids were treated and used for MS detection. The MS data were acquired using an Orbitrap Exploris 480 Mass Spectrometer coupled with an EASY-NLC 1200 liquid phase LC-MS system. DIA raw data files were analyzed by DIA-NN software. The diff...
ORGANISM(S): Homo sapiens (Human) 
2023-07-18 | PXD036119 | Pride
H157 cells were treated with nanocomposite AANL-SeNPs and harvested for mass spectrometry detection. The mass spectrum data were obtained by timsTOF Pro combined with UltiMate 3000 RSLCnano liquid chromatograph system. DIA-NN software is used to analyze DIA original data files. The differentially ex...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD044000 | Pride
Single cells from Ptenpc-/-Smad4pc-/-mTmG+ prostate tumors were isolated into single cells which were FACS-sorted for GFP+ and Tomato+ cells and RNA was purified with TRIzol (Life Technologies). RNA expression profiling was performed using the Mouse Genome 430 2.0 Array (Affymetrix) to generate a P...
ORGANISM(S): Mus musculus 
We investigated the functions of ECM1 subtypes ECM1a (inlcuding wild type and mutant) and ECM1b in ovarian cancer cell line HEYA8. While ECM1a is oncogenic, ECM1b is a tumor suppressor. To identify the molecules binding to ECM1a or ECM1b within cells, protein samples were generated by co-IP using HA...
ORGANISM(S): Homo sapiens (Human) 
2021-05-27 | PXD025239 | Pride
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