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MetabolomicsDissection of two routes to naïve pluripotency using different kinase inhibitors
Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gsk3 (2i). A relevant effect of 2i is the inhibition of Cdk8/19, which are negative regulators of the Mediator complex, responsible for the activity of enhancers. Inhibition of Cdk8/19 (Cdk8/19i) stimul...
2021-02-10 | MTBLS301 | MetaboLights
TranscriptomicsTransformants of wheat GSK3/Shaggy-like kinase TaSK5 vs wild-type Col-0
A novel cold-inducible GSK3/Shaggy-like kinase cDNA (TaSK5) was isolated from winter wheat by a macroarray-based differential screening approach. Sequence analysis of TaSK5 revealed high similarity to Arabidopsis subgroup I GSK3/Shaggy-like kinases ASK-alpha, ASK-gamma and ASK-epsilon. Transgenic Ar...
ORGANISM(S): Arabidopsis thaliana 
2014-11-02 | E-GEOD-59556 | biostudies-arrayexpress
TranscriptomicsHuman cortical organoids. Chronic treatment with GSK3 inhibitor CHIR99021. Bulk RNAseq.
Here we used human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Cortical organoids were dif...
ORGANISM(S): Homo sapiens 
2020-06-04 | E-MTAB-8325 | biostudies-arrayexpress
ProteomicsRegulation of Glycogen Synthase kinase 3beta by phosphorylation and O-beta-linked N-acetylglucosaminylation: implications on tau protein phosphorylation
Glycogen synthase kinase 3 (GSK3) plays a pivotal role in signaling pathways related to insulin metabolism and in the pathogenesis of Alzheimer s disease (AD) and other neurodegenerative disorders. More particularly, the GSK3beta isoform is involved in AD-associated tau pathology as one of the major...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-02-09 | MSV000094049 | MassIVE
ProteomicsApplication of TurboID-mediated proximity labeling and phosphoproteomics for mapping a GSK3 kinase signaling network in Arabidopsis
Transient protein-protein interactions (PPIs), such as those between posttranslational modifying enzymes and their substrates, play key roles in cellular regulation, but are difficult to identify. Here we demonstrate the application of enzyme-catalyzed proximity labeling (PL), using the engineered p...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-05-10 | PXD017085 | Pride
Proximity labeling Turboid Mass spectrometry Protein interactome Protein-protein interaction Gsk3 kinase Phosphoproteomics Plant Biotinylation 
TranscriptomicsPerturbation of the Akt/Gsk3-beta signaling pathway is common to Drosophila expressing expanded untranslated CAG, CUG and AUUCU repeat RNAs
Recent evidence supports a role for RNA as a common pathogenic agent in both the “polyglutamine” and “untranslated” dominant expanded repeat disorders. One feature of all repeat sequences currently associated with disease is their predicted ability to form a hairpin secondary structure at the RNA...
ORGANISM(S): Drosophila melanogaster 
2011-04-28 | E-GEOD-27178 | biostudies-arrayexpress
TranscriptomicsPhosphatidylinositol 3-Kinase (PI3K) Signaling via Glycogen Synthase Kinase-3 (Gsk-3) Regulates DNA Methylation of Imprinted Loci.
The two vertebrate Gsk-3 isoforms, Gsk-3a and Gsk-3b, are encoded by distinct genetic loci and exhibit mostly redundant function in murine embryonic stem cells (ESCs). Here we report that deletion of both Gsk-3a and Gsk-3b in mouse ESCs results in misregulated expression of imprinted genes and hypom...
ORGANISM(S): Mus musculus 
2010-11-15 | E-GEOD-20015 | biostudies-arrayexpress
ProteomicsRegulatory phosphorylation of eIF6 by GSK3
The overall goal of this project is to determine the key sites in the C-terminus of eIF6 that are recognized and phosphorylated by GSK3. Analysis of kinase reactions using the site-specific mutants as well as the C-terminus deletion mutants of eIF6 indicated that there are GSK3-specific motifs in th...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-06-16 | MSV000085596 | MassIVE
ProteomicsGSK3 phosphorylation sites in Lpd
Neural crest cells are multipotent cells that delaminate from the neuroepithelium, migrating throughout the embryo. Aberrant migration causes developmental defects. Animal models are improving our understanding of neural crest anomalies, but in vivo migration behaviours are poorly understood. Here, ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-05 | PXD044150 | Pride
Gsk3 Phosphorylation Cid/etd tandem mass spectrometry Lpd 
ProteomicsIdentification of phosphorylation sites and binding proteins of Saccharomyces cerevisiae Tda1
Tda1 in Saccharomyces cerevisiae is a protein kinase that is activated in response to glucose starvation. However, how it is activated has not been investigated for a long time. In this analysis, yeast cells were grown in high (2%) or low (0.05%) glucose medium, and the lysate were subjected to immu...
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2025-03-24 | PXD060707 | JPOST Repository
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