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GenomicsRNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
RNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
PRJNA561512 | ENA
ProteomicsGST Pull-down-MS identify candidate host factors that interact with CHIKV nsP4
The Chikungunya virus (CHIKV) is a single positive-stranded RNA virus transmitted by mosquitoes. More than 40 countries have reported cases of CHIKV infection to varying degrees. Currently, there is no licensed specific antiviral drug or vaccine for CHIKV in the world. In this study, we have identif...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD047753 | Pride
Chikungunya virus  nonstructural protein 4 
ProteomicsPeptidomics-guided identification of TRPV1-targeting peptides from Apis mellifera venom and macrophage proteomics
Bee venom has been traditionally used in the treatment of inflammatory disorders and is known to contain diverse bioactive peptide components. In this study, we systematically characterized the Apis mellifera venom peptidome and investigated peptides enriched under TRPV1 pull-down conditions with po...
ORGANISM(S): Mus musculus (Mouse) Apis mellifera (Honeybee) 
2026-04-17 | PXD075103 | Pride
Peptidomics Proteomics Macrophage Inflammation Apis mellifera venom Pull-down Trpv1 
ProteomicsMass spec results of Tmc7-GST pull-down
For the GST pull-down of DRG ganglion, the GST- or Tmc7-GST-associated beads were further incubated with cell lysates derived from DRG ganglion. The pulled-down proteins were then separated by SDS-PAGE gel electrophoresis and subsequently stained with silver staining Kit. Finally, the target molecul...
ORGANISM(S): Mus Musculus 
2024-03-08 | PXD050466 |
ProteomicsThe mass spectrometry raw data of proteins that interact with GRA35 in neuronal cells
To investigate the impact of Toxoplasma gondii GRA35 on neuronal cells and the underlying mechanisms, we conducted GST-pull down experiments to isolate proteins that bind to GRA35-GST and GST in neuronal cells, respectively. The dataset labeled "20240706_WJ_ GRA35_N_B01.raw" corresponds to the GRA35...
ORGANISM(S): Mus musculus (Mouse) 
2025-08-26 | PXD067653 | Pride
Neuron Gra35 Toxoplasma gondii Gst-pull down 
ProteomicsIdentification and Tetramer Structure of SPD_0310 Hemin-storage Protein Linked to Streptococcus pneumoniae Iron Homeostasis and Virulence
We found that the homologous proteins of SPD_0310 of Streptococcus pneumoniae widely exists in Gram-positive bacteria. SPD_0310 has heme binding ability. In the GST pull-down experiment, we found that it can interact with several proteins.
ORGANISM(S): Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) 
2022-08-12 | PXD030298 | Pride
Spd_0310 Gst pull-down Streptococcus pneumoniae 
ProteomicsProteomic identification of Amuc_1100-interacting proteins by GST pull-down LC–MS/MS
To identify potential interacting proteins of the outer membrane protein Amuc_1100 from Akkermansia muciniphila, GST pull-down assays were performed using lysates from HTR-8/SVneo cells, mouse placenta, and small intestine tissues. The bound proteins were analyzed by LC–MS/MS on an Orbitrap Exploris...
ORGANISM(S): Homo Sapiens Mus Musculus 
2025-11-13 | PXD070702 |
ProteomicsIdentify HILPS-interacting proteins and HIF1a phosphorylation sites
To dissect the molecular mechanisms of HILPS in promoting cell survival under hypoxia, we conducted RNA pull-down assay in combination with mass spectrometry analysis to identify HILPS-interacting proteins. Pull-down of antisense HILPS was used as a negative control. To identify the potential phosph...
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD042226 | Pride
Human Lc-ms/ms 
ProteomicsPotential binding partners of profilin protein from Leishmania donovani
For identifying potential ligands of Leishmania profilin (LdPfn) protein, full length profilin was cloned and expressed with GST tag. Only GST tag protein was used as a negative control. Employing pull down assay and with the help of LC-MS, the potential binding partners of profilin protein in Leish...
ORGANISM(S): Leishmania donovani BPK282A1 
2022-03-10 | PXD026036 | Pride
Proteomics Mass spectrometry Profilin Leishmania 
TranscriptomicsRNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
ZFP36L1 is a tandem zinc-finger RNA-binding protein that recognizes conserved Adenylate-Uridylate-rich Elements (AREs) located in 3' untranslated regions (UTRs) to mediate RNA decay. We hypothesized that ZFP36L1 is a negative regulator of a post-transcriptional hub involved in the RNA half-life regu...
ORGANISM(S): Homo sapiens 
2020-10-02 | GSE136179 | GEO
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