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ProteomicsTMEM260 is a protein-specific O-mannosyltransferase
Mutations in the TMEM260 gene cause structural heart defects and renal anomalies syndrome (SHDRA), but the function of the encoded protein remains unknown. We report that TMEM260 is an ER-located protein O-mannosyltransferase that selectively glycosylates defined extracellular immunoglobulin, plexin...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-05-05 | PXD032328 | Pride
O-mannosylation Glycoproteomics Glycopeptide Tmem260 
ProteomicsGlobal view of domain-specific O-linked mannose glycosylation in glycoengineered cells
Protein O-linked mannose (O-Man) glycosylation is an evolutionary conserved post-translational modification, whose biosynthesis is initiated by three non-redundant enzyme families, POMT1/POMT2, TMTC1-4 and TMEM260. In this study, we applied a targeted workflow for membrane glycoproteomics to five hu...
ORGANISM(S): Homo sapiens (Human) 
2024-06-10 | PXD045597 | Pride
Lc-msms O-man Glycoproteomics Ptm Glycobiology O-mannose 
ProteomicsA Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C-and O- Mannosylated Peptides
C-mannosylated peptides are easily detected by mass spectrometry (MS) from purified proteins but not from complex biological samples. Enrichment of specific glycopeptides by lectin affinity prior to MS analysis has been widely applied to support glycopeptide identification, but was up till now not a...
ORGANISM(S): Homo sapiens (Human) 
2022-05-13 | PXD031355 | Pride
Etcid Bc2l-a lectin Glycoproteomics O-mannosylation C-mannosylation 
ProteomicsMolecular-Level Architecture of Chlamydomonas reinhardtii’s Glycoprotein-Rich Cell Wall
Microalgae are a renewable and promising biomass for large-20 scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-level mode...
ORGANISM(S): Homo sapiens (Human) 
2024-01-09 | PXD048024 | Pride
Lc-msms O-man Glycoproteomics Ptm Glycoprotein Cell wall 
ProteomicsTruncated N-glycans destabilize POMT1 and POMT2 but do not limit cellular O-mannosylation in HEK293 cells
Protein O-mannosylation is initiated by the ER-resident enzymes POMT1 and POMT2, both of which carry multiple N-glycans essential for proper folding and activity. Although congenital disorders of glycosylation type I (CDG-I) disrupt the early N-glycosylation process, their impact on POMT biogenesis ...
ORGANISM(S): Homo sapiens (Human) 
2026-08-02 | PXD073937 | Pride
Alg3  pomt1  pomt2  kiaa1549  n-glycosylation  o-mannosylation 
ProteomicsKIAA1549-BRAF O-mannosylation in mNSC cells
O-Mannosylation in h9 mouse neuronal stem cells (mNSC) explored by differential O-Man glycoproteomics and BC2L-A lectin affinity chomatography
ORGANISM(S): Mus musculus (Mouse) 
2026-08-02 | PXD062030 | Pride
Kiaa1549 O-mannosylation 
ProteomicsNucleocytoplasmic O-Man glycoproteome
Dynamic cycling of N-Acetylglucosamine (GlcNAc) on serine (Ser) and threonine (Thr) residues (O-GlcNAcylation) is an essential process in all eukaryotic cells except yeast, e.g Saccharomyces cerevisiae. O-GlcNAcylation modulates signaling and cellular processes in an intricate interplay with protein...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) Schizosaccharomyces pombe 
2015-12-08 | PXD002924 | Pride
O-glycoproteomics Saccharomyces cerevisiae 
ProteomicsFruit fly O-GlcNAc glycoproteomics
The reversible posttranslational O-GlcNAc modification of serine or threonine residues of intracellular proteins is involved in many cellular events from signaling cascades to epigenetic and transcriptional regulation. O-GlcNAcylation is a conserved nutrient-dependent process involving two enzymes,...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-04-26 | PXD025344 | Pride
Fusion O-glcnac Drosophila melanogaster 
ProteomicsMapping the O-mannose glycoproteome in Saccharomyces cerevisiae
O-Mannosylation is a vital protein modification conserved from fungi to humans. In an essential step towards the full understanding of O-mannosylation we mapped the O mannose glycoproteome in baker´s yeast.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-01-18 | PXD003238 | Pride
Saccharomyces cerevisiae Glycoproteome O-mannose 
ProteomicsCHO O-Mannosylation independent of POMT1/2
Protein O-mannosylation is found in yeast and metazoans and a family of conserved orthologous polypeptide O-mannosyltransferases is believed to initiate this important posttranslational modification. We recently discovered that the large families of cadherins and protocadherins carry highly conserve...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2017-05-18 | PXD004341 | Pride
Mannose Glycoproteomics O-mannosyltransferase O-glycosylation 
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