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Proteomics: Proteomic analysis of Nicotiana benthamiana upon infection of grapevine fanleaf virus strains
Nicotiana benthamiana was infected with several strains of grapevine fanleaf virus (GFLV). Apical tissue was collected 4, 7, and 12 days after inoculation, with identical samples for shotgun proteomics and transcriptomics analysis. Five leaf discs per leaf were collected a pooled by three plants int...
ORGANISM(S): Nicotiana benthamiana 
2023-07-20 | PXD037185 | Pride
Tobacco Virus Grapevine Plant Lc-msms 
ProteomicsAsian citrus psyllid proteomics: Shotgun MS/MS analysis of impact of plant host on insect proteome + DIGE analysis of impact of plant host on insect proteome
The Asian citrus psyllid (Diaphorina citri) is a pest of citrus and the primary insect vector of the bacterial pathogen, ‘Candidatus Liberibacter asiaticus’ (CLas), which is associated with citrus greening disease. Variability in CLas titer in insects collected from infected plants has been attribut...
ORGANISM(S): Diaphorina citri (Asian citrus psyllid) 
2022-04-27 | PXD011401 | Pride
Asian citrus psyllid Citrus greening Diaphorina citri Insect vector Candidatus liberibacter asiaticus Huanglongbing 
ProteomicsDifferentially expressed proteins wereassociated withtwo color morphs of Asian citrus psyllid, Diaphorina citri
Diaphorina citriis a vector of ‘CandidatusLiberibacter asiaticus,’(CLas), associated with citrus greening or Huanglongbing (HLB) disease in citrus. D. citriexhibits two group three different color morph variants, blueand non-blue (includes gray and yellow). Blue morphs have a greater capacity fo...
ORGANISM(S): Diaphorina citri (Asian citrus psyllid) 
2021-04-26 | PXD017150 | Pride
Color polymorphism Immunity Proteomics survey 
ProteomicsIsolation of aphid-Potato leafroll virus (PLRV) proteins complexes from viruliferous insects using affinity purification-mass spectrometry
The control of insect borne disease is recognized as one of the major agricultural, animal and human health challenges of today. Viruses in the family Luteoviridae are phloem-limited, plant viruses that are vectored by aphids in a circulative manner. They are responsible for a wide-range of economic...
ORGANISM(S): Potato leafroll virus Myzus persicae 
2021-05-27 | PXD022167 | Pride
Aphid Phloem-limited plant virus Ap-ms. Potato pathogen Potato leafroll virus Plrv Luteoviridae Polerovirus Circulative transmission Myzus persicae 
ProteomicsProteomic analysis of various Bemisia tabaci population with variable TYLCV transmission abilities
Begomoviruses, the largest, most damaging and emerging group of plant viruses in the world, infect hundreds of plant species and new virus species of the group are discovered each year. They are transmitted by species of the whitefly Bemisia tabaci. Tomato yellow leaf curl virus (TYLCV) is one of th...
ORGANISM(S): Bemisia tabaci 
2020-10-26 | PXD016964 | Pride
Bemisia tabaci Tylc Transmission Bacterial symbiont 
ProteomicsAsian citrus psyllid hemolymph LC-MS/MS
Citrus greening disease, also known as Huanglongbing (HLB), is the most serious disease of citrus plants. It is associated with the Gram-negative bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), which is transmitted between host plants by the hemipteran insect vector Diaphorina citri in a circu...
ORGANISM(S): Diaphorina citri (Asian citrus psyllid) 
2018-08-16 | PXD009257 | Pride
Hemolymph Vitellogenin Diaphorina citri Citrus Psyllid Candidatus liberibacter asiaticus Lc-ms/ms 
ProteomicsAptamer Affinity Mass Spectrometry
Antibody-based affinity purification of macromolecular complexes has revolutionized the study of protein-protein interactions. Here, we present AptA-MS (Aptamer Affinity – Mass Spectrometry), a robust strategy using a specific, high-affinity RNA aptamer against GFP to identify novel interactors of a...
ORGANISM(S): Homo sapiens (Human) 
2020-06-17 | PXD015620 | Pride
Hct116 Aptamer Rna aptamer Heat shock factor S2 
ProteomicsPeptidomics of the Asian Citrus Psyllid
The aim of this study was to develop methods for the extraction of endogenous peptides from the Asian citrus psyllid, vector of the bacteria that cause the major citrus disease Huanglongbing. In addition, the native peptidome of the Asian citrus psyllid is described, with an emphasis on candidate ne...
ORGANISM(S): Diaphorina citri (Asian citrus psyllid) 
2020-03-19 | PXD014801 | Pride
Neuropeptides Peptidome Psyllid 
ProteomicsBacteria Associated with Russian Wheat Aphid (Diuraphis noxia) Enhance Aphid Virulence to Wheat
Phenotypic responses to biotic stresses are often studied as the interactions between two species; however, in the phytobiome, these responses frequently result from complex interactions involving several organisms. Here, we show that variation in chlorosis caused by Russian wheat aphid (Diuraphis n...
ORGANISM(S): Diuraphis noxia 
2020-11-09 | PXD010663 | Pride
Aphid saliva | proteome | enterobacteriaceae | multitrophic interactions | microbiome | phytobiome 
ProteomicsEvaluation of a bead-free immunoprecipitation technique coupled with tandem mass spectrometry for identification of plant-virus protein interactions (DeBlasio_JBT_2017)
Potato leafroll virus (PLRV) is an aphid-borne, RNA virus in the Luteoviridae that causes significant loss to potato production worldwide. Precision methods to block virus infection in plants or the transmission of a virus by the insect represent new approaches to virus disease management and requir...
ORGANISM(S): Nicotiana benthamiana 
2018-10-24 | PXD006338 | Pride
Host-pathogen interactions Bead-free immunoprecipitation  plant-virus interactome  tandem mass spectrometry Luteoviridae Polerovirus Molecular virology Potato leafroll virus Phloem-limited 
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