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ProteomicsProteins associated with chromatin in Hela cells detected by LC-MS
Chromatin fraction was seperated from Hela cells. Proteins associated with chromatin was detected by LC-MS.
ORGANISM(S): Homo sapiens (Human) 
2024-11-15 | PXD053976 | Pride
Hela chromatin lc-ms 
ProteomicsAnalysis of protein-DNA interactions in chromatin by UV induced cross-linking and mass spectrometry
Protein–DNA interactions are key to the functionality and stability of the genome. Identification and mapping of protein–DNA interaction interfaces and sites is crucial for understanding DNA-dependent processes. Here, we present a workflow that allows mass spectrometric (MS) identification of protei...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2020-10-20 | PXD020290 | Pride
Mass spectrometry Protein-dna interactions Uv-cross-linking 
ProteomicsProteomic profiling reveals distinct phases to the restoration of chromatin following DNA replication
Chromatin organization must be maintained during cell proliferation to preserve cellular identity and genome integrity. However, DNA replication results in transient displacement of DNA bound proteins, and it is unclear how they regain access to newly replicated DNA. Using quantitative MS-based prot...
ORGANISM(S): Homo sapiens (Human) 
2023-02-02 | PXD038113 | Pride
Proteomics Lc-ms Tmt 
ProteomicsLC-MS/MS analysis of histone modifications in IR-treated HeLa cells
To systematically identify individual and combinatorial chromatin modifications involved in early DDR, we performed PTM assessments by using unbiased, label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) with histones extracted from irradiation (IR)-treated Hela cells and the matched...
ORGANISM(S): Homo Sapiens 
2023-07-03 | PXD043381 |
ProteomicsxIP-MS: topological analysis of chromatin-associated protein complexes using single affinity purification
In recent years, cross-linking mass spectrometry (XL-MS) has proven to be a robust and effective method of interrogating macromolecular protein complex topologies at peptide resolution. Traditionally, XL-MS workflows have utilized homogenous complexes obtained through time-limiting reconstitution, t...
ORGANISM(S): Homo sapiens (Human) 
2015-11-17 | PXD002987 | Pride
Affinity purification Chemical cross-linking mass-spectrometry Chromatin 
ProteomicsSUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage
Small Ubiquitin-like Modifiers play critical roles in the DNA Damage Response (DDR). To increase our understanding of SUMOylation in the mammalian DDR, we employed a quantitative proteomics approach to identify dynamically regulated SUMO-2 conjugates and modification sites upon treatment with the DN...
ORGANISM(S): Homo sapiens (Human) 
2015-03-16 | PXD001810 | Pride
Dna Damage Mms Sumo-2 Sumo Chromatin Repair Site 
ProteomicsThe SETD3 interacting protein was identified and screened by TurboID method, and Search for potential histidine methylation modification sites
we employed a TurboID approach that utilized a modified promiscuous biotin ligase BirA for proximity labeling by fusing SETD3 with this enzyme, By introducing biotin, cellular fractions of SETD3-TurboID-transfected cells, both cytosolic and chromatin fractions, underwent immunoprecipitation, followe...
ORGANISM(S): Homo sapiens (Human) 
2024-03-12 | PXD046472 | Pride
Turboid Hela s3 Lc-ms/ms 
ProteomicsMethylome profiling of SetDB1 deficient ESCs reveals coordinated epigenetic cross-talk during pluripotency
SetDB1 is best known as a chromatin modifier catalyzing H3K9me3. However, recent studies show that SetDB1 can mediate H3K27me3-deposition by PRC2, CTCF-binding, recruit DNA methyltransferases, and potentially regulate additional chromatin processes. To gain further insights, we profiled the protein ...
ORGANISM(S): Homo sapiens (Human) 
2026-08-20 | PXD074363 | Pride
Hela Pulldown Epigenetics Co-immunoprecipitation Nuclear extract Gfp Chromatin Setdb1 
ProteomicsThe OGT TPR Interactome defined by BioID
BioID performed in HeLa cells for the TPR domain of The O-GlcNAc Transferase and eGFP (negative control). Used to determine TPR interactors and the TPR interactome in HeLa cells. Three biological replicates were performed, with each replicate having an experimental (TPR-BirA*) and negative (eGFP-Bir...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-06-24 | MSV000085626 | MassIVE
ProteomicsOnline Weak Cation Exchange-HILIC × Reversed Phase LC UVPD-HRMS of intact HeLa Histones
Intact HeLa core Histones were analyzed using an online two-dimensional liquid chromatographic separation as well as one dimensional LC (RPLC and WCX-HILIC) coupled with UVPD-MS
ORGANISM(S): Homo sapiens (Human) 
2018-09-19 | PXD008978 | Pride
Histones Top-down proteomics Online 2dlc Uvpd 
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