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ProteomicsComparison non-targeting hRNA hESCs versus UBE2K shRNA hESCs
Histones modulate gene expression by chromatin compaction, regulating numerous processes such as differentiation. However, the mechanisms underlying histone degradation remain elusive. When compared with their differentiated counterparts, immortal human embryonic stem cells (hESCs) have a unique chr...
ORGANISM(S): Homo sapiens (Human) 
2020-06-01 | PXD018625 | Pride
Histones Ubiquitin proteasome system Hescs 
ProteomicsTranslational and Post-translational LC-MS of Naïve and Primed hESCs
Deciphering the regulatory network for human naïve and primed pluripotency is of fundamental theoretical and applicable significance. Here, by combining quantitative proteomics, phosphoproteomics and acetylproteomics analyses, we revealed RNA processing and translation as the most differentially-reg...
ORGANISM(S): Homo sapiens (Human) 
2021-12-10 | PXD030230 | Pride
Lc-ms Primed Human Hesc Naïve 
MetabolomicsPRODH regultes naive pluripotency by influencing mitochondrial oxidative phosphorylation (Untargeted metabolomics)

Naïve human embryonic stem cells (hESCs) that resemble the pre-implantation epiblasts are fueled by a combination of aerobic glycolysis and oxidative phosphorylation, but their mitochondrial regulators are poorly understood. Here we report that, proline dehydrogenase (PRODH), a mitochondria-local...

2024-02-12 | MTBLS7840 | MetaboLights
MetabolomicsPRODH regultes naive pluripotency by influencing mitochondrial oxidative phosphorylation (Targeted metabolomics)

Naïve human embryonic stem cells (hESCs) that resemble the pre-implantation epiblasts are fueled by a combination of aerobic glycolysis and oxidative phosphorylation, but their mitochondrial regulators are poorly understood. Here we report that, proline dehydrogenase (PRODH), a mitochondria-local...

2024-02-12 | MTBLS7832 | MetaboLights
MetabolomicsThe functional role of OGDH for maintaining mitochondrial respiration and identity of primed human embryonic stem cells

Human embryonic stem cells (hESCs) can self-renew infinitely or differentiate into the 3 germ layer lineages in vitro with certain cues, holding great promise to model early human embryo development ex vivo. It is wildly accepted that hESCs take advantage of both glycolysis and ...

2022-03-03 | MTBLS4223 | MetaboLights
ProteomicsA post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells
While the transcriptional network of human embryonic stem cells (hESCs) has been extensively studied, relatively little is known about how post-transcriptional modulations determine hESC function. RNA-binding proteins play central roles in RNA regulation, including translation and turnover. Here we ...
ORGANISM(S): Homo sapiens (Human) 
2018-05-16 | PXD007270 | Pride
Shrna Lc-ms Hescs 
ProteomicsCo-immumnoprecipitation experiments to identify protein bindining partners of CSDE1
While the transcriptional network of human embryonic stem cells (hESCs) has been extensively studied, relatively little is known about how post-transcriptional modulations determine hESC function. RNA-binding proteins play central roles in RNA regulation, including translation and turnover. Here we ...
ORGANISM(S): Homo sapiens (Human) 
2018-05-16 | PXD007271 | Pride
Lc-ms Co-ip Hescs 
ProteomicsMetabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis
A hallmark of cancer cells is the metabolic switch from oxidative phosphorylation (OXPHOS) to glycolysis, a phenomenon referred to as the “Warburg effect”, which is also observed in primed human pluripotent stem cells (hPSCs). Here, we report that downregulation of SIRT2 and upregulation of SIRT1 is...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD006036 | Pride
Acetylation Sirt2 Differentiation Human pluripotent stem cells 
ProteomicsTo functionally investigate the manner that UBQLN1 regulate mitochondria function,we searched for the UBQLN1-interacting proteins,we first conducted LC-MS with 3Flag-UBQLN1 cell line
To functionally investigate the manner that UBQLN1 regulatr mitochondria function,we searched for UBQLN1-interacting proteins,we conduct LC-MS with 3flag-UBQLN1 cell line
ORGANISM(S): Homo sapiens (Human) 
2024-07-03 | PXD036634 | Pride
Hescs lc-ms 
TranscriptomicsALKBH1 deficiency leads to loss of homeostasis in human diploid somatic cells
DNA N6-methyladenosine (6mA) in eukaryotic genomes has recently been observed in diverse species. 6mA has been reported to associate with multiple physiological processes including embryonic development and tumorigenesis, and ALKBH1 is a primary 6mA demethylase in mouse and human cells. To research ...
ORGANISM(S): Homo sapiens 
2020-05-22 | GSE150936 | GEO
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