Sort   by:  
 Page size 
ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
Membrane proteins are crucial targets for cancer biomarker discovery and drug development. However, in addition to the inherent challenges of hydrophobicity and low abundance, complete membrane proteome coverage of clinical specimen is usually hindered by the requirement of large amount of starting ...
ORGANISM(S): Rattus rattus (Black rat) Homo sapiens (Human) 
2015-11-16 | PXD001548 | Pride
High-ph reverse phase stagetip Membrane proteins Lung cancer 
ProteomicsMining Missing Membrane Proteins by High-pH Reverse Phase StageTip Fractionation and Validation by Multiple Reaction Monitoring Mass Spectrometry
Several significant efforts have been made in the past decade to map all human proteome. However, more than 3000 proteins are grouped as missing having no evidence of translation. Membrane protein annotation of the current missing proteins list showed 34%to be missing membrane proteins, which are ch...
ORGANISM(S): Homo sapiens (Human) 
2015-07-27 | PXD002224 | Pride
High-ph reverse phase Stagetip Missing proteins Lung cancer 
ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showi...
ORGANISM(S): Homo Sapiens (human) 
2016-09-08 | PXD004904 | JPOST Repository
Proteomicsmouse microglia using High-pH fractionation and LC-MS/MS
To build a reference proteome, we established a BV-2 microglial proteome to a depth of 5494 unique protein groups using a novel strategy that combined FASP, StageTip-based high pH fractionation, and high-resolution mass spectrometry quickly and cost-efficiently. By bioinformatics analysis, the BV-2 ...
ORGANISM(S): Mus musculus (Mouse) 
2013-09-11 | PXD000168 | Pride
Mouse High-ph fractionation Microglia Lc-ms/ms 
UnknownMining Missing Membrane Proteins by High-pH Reverse-Phase StageTip Fractionation and Multiple Reaction Monitoring Mass Spectrometry.
Not available
S-EPMC4761227 | biostudies-literature
ProteomicsIn-depth proteomic analysis of secretome and whole cells of mouse astrocyte
In this study, 3 biological replicates with 3 technical replicates for the conditioned media (CM) and the whole cell lysates (WCL) of C8-D1A cell line were analyzed using 108 LC-MS/MS runs. Each peptide sample was separated into 6 fractions using stageTip based High-pH fractionation. The peptide sam...
ORGANISM(S): Mus musculus (Mouse) 
2014-06-05 | PXD000501 | Pride
Mouse Whole cell proteome High-ph fractionation Secretome Astrocyte Lc-ms/ms 
ProteomicsIn-depth proteome analysis of human ascites using high-pH peptide fractionation
For in-depth profiling, ascites from patients with benign disease or gastric cancer were subjected to high-resolution mass spectromtery analysis. By high-pH peptide fractionation, 3481 protein groups were identified in 72 LC-MS/MS runs.
ORGANISM(S): Homo sapiens (Human) 
2018-04-16 | PXD003351 | Pride
Ascites High-ph peptide fractionation Gastric cancer 
ProteomicsMembrane Proteome Profiling of Peripheral Immune Cells in Non-Small Cell Lung Cancer Patients Treated with First Line Combination Chemotherapy
Four lung adenocarcinoma patients with progression free survival (PFS) ranged from 2.13 to 14.5 months were recruited in the study. Paired PBMCs were collected before and after first regimen of PEM/CIS treatment for individualized analysis of membrane proteome profiles. The membrane protein fraction...
ORGANISM(S): Homo sapiens (Human) 
2018-01-02 | PXD006297 | Pride
Immune cell profiling Llung adenocarcinoma Membrane proteomics 
ProteomicsEffects of lipid diets on the metaproteome of mouse colon mucosa
Eight mice colon samples were submitted for iTRAQ labeling, high pH reversed phase fractionation and LC-MS/MS analysis.
ORGANISM(S): Mus musculus (Mouse) Bacteroidetes Firmicutes bacterium 
2019-02-20 | PXD008152 | Pride
Fatty acids Lipid diet Metaproteome Mouse Itraq Colon mucosa 
ProteomicsEffects of lipid diets on the metaproteome of the mice colon submucosa
Eight mice colon (submucosa) samples were submitted for iTRAQ labeling, high pH reversed phase fractionation and LC-MS/MS analysis.
ORGANISM(S): Mus musculus (Mouse) Bacteroidetes Firmicutes MN01-01 
2019-02-20 | PXD008165 | Pride
Lipid diet Metaproteome Submucosa Mice Colon Itraq 
Sort   by:  
 Page size