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ProteomicsIdentification of photosystem I components from Chlamydomonas reinhardtii grown under oxic and anoxic conditions
Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here we reveal a chloroplast PSI from the...
ORGANISM(S): Chlamydomonas reinhardtii 
2023-03-11 | PXD027067 | Pride
Photosystem i Anoxia Blue native page Psi Chlamydomonas reinhardtii 
ProteomicsAltered N-glycan composition impacts flagella mediated adhesion in Chlamydomonas reinhardtii
For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion. However, it is not known whether only the presence or also the composition of N-glycans a...
ORGANISM(S): Chlamydomonas reinhardtii 
2020-12-22 | PXD018353 | Pride
Adhesion Sugarpy Prm Chlamydomonas reinhardtii N-glycosylation Flagella Lc-ms/ms 
ProteomicsThe red alga Porphyridium as a host for molecular farming: Efficient production of immunologically active hepatitis C virus glycoprotein
Microalgae are promising production platforms for the cost-effective production of recombinant proteins. We have recently established that the red alga Porphyridium purpureum provides superior transgene expression properties, due to the episomal main- tenance of transformation vectors as multicopy p...
ORGANISM(S): Porphyridium purpureum 
2024-05-21 | PXD052401 | Pride
N-glycosylation Hcv Porphyridium 
ProteomicsPhosphoproteomic analysis of Chlamydomonas PetD mutants
The cytochrome b6f complex (b6f) links photosystem II and photosystem I in photosynthetic electron transfer and is distributed between appressed and non-appressed thylakoid membranes. It also activates the state-transition 7 kinase (STT7), which phosphorylates light-harvesting complex proteins, faci...
ORGANISM(S): Chlamydomonas reinhardtii 
2026-04-04 | PXD060640 | Pride
Petd Chlamydomonas Phosphorylation Stt7 
ProteomicsPhosphoproteomic analysis of the high light response of Chlamydomonas reinhardtii
Characterization of the phosphoproteome of Chlamydomonas reinhardtii before and 24h after the transition from normal to high light.
ORGANISM(S): Chlamydomonas reinhardtii 
2023-02-21 | PXD036166 | Pride
Photosystems Photosynthesis Phosphorylation Chlamydomonas 
ProteomicsChemical crosslinking of plastocyanin and the cytochrome b6f complex
In this work we investigated the role of plastocyanin (PC) phosphorylation in photosynthetic electron transfer, focusing on PC's interaction with both cytochrome b6f, (Cytb6f) and photosystem I (PSI) in Chlamydomonas reinhardtii. While the binding and electron transfer between PC and PSI are well ch...
ORGANISM(S): Chlamydomonas reinhardtii 
2025-08-25 | PXD060078 | Pride
Cytochrome b6f Plastocyanin Chlamzdomonas Edc Crosslinking 
ProteomicsTemporal Changes in the Chlamydomonas Proteome in Response to High Light
Quantitative proteomic analysis of Chlamydomonas reinhardtii exposed to high light. Samples were taken 0h, 1h, 4h and 24h after beginning of the treatment.
ORGANISM(S): Chlamydomonas reinhardtii 
2019-04-23 | PXD010160 | Pride
Algae High light Q exactive plus Label-free quantification Chlamydomonas 
ProteomicsChemical crosslinking of photosystem I antenna proteins
Chemical crosslinking (DSS) of proteins in conjunction with mass spectrometry was performed to identify protein-protein interactions between the light-harvesting proteins of PSI and PSII.
ORGANISM(S): Chlamydomonas reinhardtii 
2025-04-29 | PXD051493 | Pride
Photosynthesis Lhc Chlamydomonas Light harvesting 
ProteomicsSugarPy facilitates the universal, discovery-driven analysis of intact glycopeptides
Protein glycosylation is a complex post-translational modification with crucial cellular functions in all domains of life. Currently, large-scale glycoproteomics approaches rely on glycan database dependent algorithms and are thus unsuitable for discovery-driven analyses of glycoproteomes. Therefore...
ORGANISM(S): Chlamydomonas reinhardtii Haloferax volcanii (Halobacterium volcanii) Homo sapiens (Human) Cyanidioschyzon merolae 
2021-03-09 | PXD017345 | Pride
Glycosylation Glycoproteomics Microalgae Archaea Sugarpy Is-cid Human breast milk Computational proteomics Lc-ms/ms 
ProteomicsChlamydomonas reinhardtii quantification of Mannosidase 1A and Xylosyltransferase1A
We have identified insertional mutants of mannosidase 1A and xylosyltransferase 1A in Chlamydomonas reinhardtii. To verify a knockout/knockdown of the enzymes in the insertional mutant strains, PRM measurements were performed. Mass spectrometric analyses of intact N-glycopeptides revealed that disru...
ORGANISM(S): Chlamydomonas reinhardtii 
2018-02-27 | PXD005257 | Pride
Chlamydomonas reinhardtii N-glycosylation Quantification 
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