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ProteomicsIdentification of photosystem I components from Chlamydomonas reinhardtii grown under oxic and anoxic conditions
Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here we reveal a chloroplast PSI from the...
ORGANISM(S): Chlamydomonas reinhardtii 
2023-03-11 | PXD027067 | Pride
Photosystem i Anoxia Blue native page Psi Chlamydomonas reinhardtii 
ProteomicsThe red alga Porphyridium as a host for molecular farming: Efficient production of immunologically active hepatitis C virus glycoprotein
Microalgae are promising production platforms for the cost-effective production of recombinant proteins. We have recently established that the red alga Porphyridium purpureum provides superior transgene expression properties, due to the episomal main- tenance of transformation vectors as multicopy p...
ORGANISM(S): Porphyridium purpureum 
2024-05-21 | PXD052401 | Pride
N-glycosylation Hcv Porphyridium 
ProteomicsPhosphoproteomic analysis of the high light response of Chlamydomonas reinhardtii
Characterization of the phosphoproteome of Chlamydomonas reinhardtii before and 24h after the transition from normal to high light.
ORGANISM(S): Chlamydomonas reinhardtii 
2023-02-21 | PXD036166 | Pride
Photosystems Photosynthesis Phosphorylation Chlamydomonas 
ProteomicsChemical crosslinking of plastocyanin and the cytochrome b6f complex
In this work we investigated the role of plastocyanin (PC) phosphorylation in photosynthetic electron transfer, focusing on PC's interaction with both cytochrome b6f, (Cytb6f) and photosystem I (PSI) in Chlamydomonas reinhardtii. While the binding and electron transfer between PC and PSI are well ch...
ORGANISM(S): Chlamydomonas reinhardtii 
2025-08-25 | PXD060078 | Pride
Cytochrome b6f Plastocyanin Chlamzdomonas Edc Crosslinking 
ProteomicsSugarPy facilitates the universal, discovery-driven analysis of intact glycopeptides
Protein glycosylation is a complex post-translational modification with crucial cellular functions in all domains of life. Currently, large-scale glycoproteomics approaches rely on glycan database dependent algorithms and are thus unsuitable for discovery-driven analyses of glycoproteomes. Therefore...
ORGANISM(S): Chlamydomonas reinhardtii Haloferax volcanii (Halobacterium volcanii) Homo sapiens (Human) Cyanidioschyzon merolae 
2021-03-09 | PXD017345 | Pride
Glycosylation Glycoproteomics Microalgae Archaea Sugarpy Is-cid Human breast milk Computational proteomics Lc-ms/ms 
ProteomicsChemical crosslinking of photosystem I antenna proteins
Chemical crosslinking (DSS) of proteins in conjunction with mass spectrometry was performed to identify protein-protein interactions between the light-harvesting proteins of PSI and PSII.
ORGANISM(S): Chlamydomonas reinhardtii 
2025-04-29 | PXD051493 | Pride
Photosynthesis Lhc Chlamydomonas Light harvesting 
ProteomicsChlamydomonas reinhardtii quantification of Mannosidase 1A and Xylosyltransferase1A
We have identified insertional mutants of mannosidase 1A and xylosyltransferase 1A in Chlamydomonas reinhardtii. To verify a knockout/knockdown of the enzymes in the insertional mutant strains, PRM measurements were performed. Mass spectrometric analyses of intact N-glycopeptides revealed that disru...
ORGANISM(S): Chlamydomonas reinhardtii 
2018-02-27 | PXD005257 | Pride
Chlamydomonas reinhardtii N-glycosylation Quantification 
ProteomicsTemperature induced remodeling of the photosynthetic machinery of the red alga Cyanidioschyzon merolae
Cyanidioschyzon merolae is a thermophilic red alga with an optimum growth temperature of 42°C. In this study we investigated the acclimation process of the alga to a colder temperature (25°C). To this aim we performed quantitative proteomic analyses of whole cells as well as solubilized thylakoid pr...
ORGANISM(S): Cyanidioschyzon merolae 
2017-04-03 | PXD005615 | Pride
Red algae Photosystems Acclimation Cyanidioschyzon merolae 
ProteomicsPhosphorylation of RBOHF by CIPK11 and CIPK26 in vitro
RBOHF from Arabidopsis thaliana represents a multifunctional NADPH oxidase regulating biotic and abiotic stress tolerance, developmental processes and guard cell aperture. The molecular components and mechanisms determining RBOHF activity remain to be elucidated. While the Ca2+ sensor-activated kina...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-11-06 | PXD011587 | Pride
Arabidopsis thaliana Cipk Calcium Phosphorylation Rbohf Aba 
ProteomicsComparison of wildtype and gr1 knock-out mutants of Physcomitrella patens using quantitative proteomics
(1) While it is known that redox-regulation in chloroplasts is vital for functional photosynthesis, the interplay of different redox-cascades in balancing reactive oxygen species (ROS) with metabolic regulation via thiol switching is still partly unresolved. We investigate the importance of the glut...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2020-05-06 | PXD012843 | Pride
Glutathion reductase Light response Physcomitrella patens Gr1 
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