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ProteomicsAntagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest
The seminal description of cellular restriction factor APOBEC3G and its antagonism by HIV-1 Vif has underpinned two decades of research on the host-virus interaction. As well as APOBEC3G and its homologues, however, we have recently discovered that Vif is also able to degrade the PPP2R5 family of re...
ORGANISM(S): Homo sapiens (Human) 
2020-04-17 | PXD018271 | Pride
Hiv Pp2a Proteomics Vif 
ProteomicsARIH2 is a novel Vif-dependent regulator of CUL5-mediated APOBEC3G degradation in HIV infection
Discovery of novel host-virus interactions leads to a better understanding of mechanisms underlying infection and points to potential therapeutic targets at the interface between virus and host proteins. Recently, global, virus-host interaction networks have been mapped using affinity purification-m...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-29 | MSV000084855 | MassIVE
ProteomicsARIH2 is a novel Vif-dependent regulator of CUL5-mediated APOBEC3G degradation in HIV infection
Discovery of novel host-virus interactions leads to a better understanding of mechanisms underlying infection and points to potential therapeutic targets at the interface between virus and host proteins. Recently, global, virus-host interaction networks have been mapped using affinity purification-m...
ORGANISM(S): Homo sapiens (Human) 
2019-04-11 | PXD009012 | Pride
Ap-ms Host-virus interactions Cullin ring e3 ligases Arih2 Cul5 Hiv 
ProteomicsTemporal proteomic analysis of HIV-Infection Reveals Remodelling of the Cellular Phosphoproteome by Phylogenetically Diverse Lentiviral Vif Variants
HIV accessory proteins manipulate host factors to evade cellular restriction and enhance viral replication. We used multiplex tandem mass tag (TMT)-based whole cell proteomics to gain a comprehensive, time-based overview of proteins and processes subverted during HIV infection. To make specific func...
ORGANISM(S): Homo sapiens (Human) 
2016-09-29 | PXD004187 | Pride
Hiv Whole cell proteome Tmt Temporal 
GenomicsCBFb Stabilizes HIV Vif to Counteract APOBEC3 at the Expense of RUNX1 Target Gene Expression [ChIP-seq]
The HIV-1 accessory protein Vif hijacks a cellular Cullin-RING ubiquitin ligase, CRL5, to promote degradation of the APOBEC3 (A3) family of restriction factors. Recently, the cellular transcription cofactor CBFb was shown to form a complex with CRL5-Vif and to be essential for A3 degradation and vir...
ORGANISM(S): Homo sapiens 
2013-01-17 | E-GEOD-42575 | biostudies-arrayexpress
TranscriptomicsTat RNA silencing suppressor activity contributes to perturbation of lymphocyte miRNAs by HIV-1
Herein expression trends of host miRNA were measured in CEMx174 lymphocytes infected with HIV-1NL4-3 or derivative strains deficient in Tat RSS activity (RSS) or vif-/vpr (VV), or unexposed to virus. As expected from previous studies, miRNA trends were different in naive and HIV-1 infected cells. ...
ORGANISM(S): Homo sapiens 
2011-03-01 | E-GEOD-21892 | biostudies-arrayexpress
ProteomicsProbing the HIV-Host Interactome in Infected Cells
Although genetically compact, HIV-1 commandeers vast arrays of cellular machinery that sustain and protect it during cycles of viral outgrowth. Transposon-mediated saturation linker scanning mutagenesis was used to isolate fully replication-competent viruses harboring a potent foreign epitope tag. U...
ORGANISM(S): Homo sapiens (Human) 
2016-07-06 | PXD003762 | Pride
Protein interactions Ap-ms Env I-dirt Vif 
TranscriptomicsMiSeq 2x300 paired-end sequencing of HEK293T cells stably expressing eGFP-PPP2R5A transduced with a HIV-1 Vif mutant library
The goal of this experiment was to map the binding surface between the HIV-1 Vif protein and the cellular phospho-regulator PPP2R5A. Previous studies had shown that Vif can target PPP2R5A for proteasomal degradation but the binding surface required had yet to be elucidated. For this experiment, HEK2...
ORGANISM(S): Homo sapiens 
2019-10-01 | E-MTAB-8357 | biostudies-arrayexpress
ProteomicsA3G-Vif-CBFB-CRL5 Cross-Linking with DSSO
Here we use an optimization cross-linking mass spectrometry (XL-MS) pipeline for the structural characterization of a dynamic HIV-host protein complex. Using DSSO-based XL-MS analysis, residue-protein proximity restraints based on functional genetics, and integrative modeling, we define the structu...
ORGANISM(S): Homo sapiens (Human) Human immunodeficiency virus 
2021-11-03 | PXD025391 | Pride
Xl-ms Dsso Apobec3g Hiv vif Integrative modeling 
ProteomicsHIV-1 infection induces Vif-mediated SUMOylation of host RNA splicing factors important for proper viral RNA splicing
HIV-1 exploits host cell post-translation modifications (PTMs) to facilitate production of infectious particles. These modifications include SUMOylation, a dynamically regulated PTM involving covalent attachment of small ubiquitin-like modifiers (SUMOs) to lysine (K) residues of target proteins. SUM...
ORGANISM(S): Homo sapiens (Human) 
2026-04-28 | PXD075554 | Pride
Rna splicing Hiv Sumoylation 
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