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ProteomicsCombined 15N-Labelling and TandemMOAC Quantifies Phosphorylation of MAP Kinase Substrates Downstream of MKK7 in Arabidopsis
Reversible protein phosphorylation is a widespread posttranslational modification that plays a key role in eukaryotic signal transduction. Due to the large dynamic range of protein abundance, low stoichiometry and transient nature of protein phosphorylation, the detection and accurate quantification...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-01-02 | PXD008200 | Pride
Phosphoproteomics Tandem moac 15n labeling Mapk Mpk substrate 
ProteomicsAssessment of Label-Free Quantification in Discovery Proteomics and Impact of Technological Factors and Natural Variability of Protein Abundance
Proteome wide protein quantification has become facile in the last years supported by label-free discovery proteomics approaches and powerful, easy to use software. We set out to independently evaluate this highly peptide centric technology focusing on the performance of some of the currently most p...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-03-07 | PXD004025 | Pride
Arabidopsis Label-free Protein inference Progenesis qip Shotgun proteomics Maxquant Epigenetics Scaffold 
ProteomicsProteomics of Tomato Trichome Metabolism
Glandular trichomes (GT) are specialized cell factories that have the capacity to produce large amounts of metabolites which can amount to over 10% of the leaf dry weight. The specific expression of secondary metabolite pathways in glandular trichomes has facilitated their elucidation. However, litt...
ORGANISM(S): Solanum lycopersicum 
2017-07-04 | PXD003154 | Pride
Trichomes Tomato 
ProteomicsVariation in auxin sensing guides AUX/IAA repressor ubiquitylation and destruction
Auxin is a drug-like small molecule and morphogen that triggers the formation of SCFTIR1/AFB-AUX/IAA co-receptor complexes leading to ubiquitylation and proteasome-dependent degradation of AUX/IAA transcriptional repressors in plants. Here, we systematically dissect auxin sensing by SCFTIR1-IAA6 and...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-06-14 | PXD004027 | Pride
Phytohormone Ubiquitylation Auxin Scf-type e3 ligase Ubiquitination Tir1/afbs Proteolysis Aux/iaa 
ProteomicsDegron flexibility enforces auxin sensing
Cullin RING-type E3 ubiquitin ligase SCFTIR1/AFB1-5 and their ubiquitylation targets, AUX/IAAs, sense auxin concentrations in the nucleus. TIR1 binds a surface- exposed degron in AUX/IAAs promoting their ubiquitylation and rapid auxin-regulated proteasomal degradation. Here, we resolved TIR1·auxin·I...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-05-27 | PXD015392 | Pride
Degron Auxin Phytohormones Aux/iaa Scftir1 Intrinsic disorder Allostery 
ProteomicsComparative Transcriptomics and Proteomics on Pi Depleted Arabidopsis Roots
Plant adaptation to limited phosphate (Pi) availability comprises a wide range of strategies to conserve and remobilize internal Pi stores and to enhance Pi acquisition. Vigorous restructuring of root system architecture provides a developmental strategy for topsoil exploration and Pi scavenging. Ch...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-05-06 | PXD003449 | Pride
Arabidopsis Phosphate Root Proteomics 
ProteomicsTargeted Proteomics of NERD and FLS2 on LTQ-Orbitrap Velos Mass Spectrometer
Targeted proteomics has become increasingly popular recently because of its ability to precisely quantify selected proteins in complex cellular backgrounds. We demonstrate the utility of an LTQ-Orbitrap Velos Pro mass spectrometer in targeted parallel reaction monitoring (PRM) despite its unconventi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-12-09 | PXD001119 | Pride
Ltq-orbitrap Prm Fls2 Targeted proteomics Nerd 
ProteomicsAccumulation of small molecules and proteins in Arabidopsis thaliana root exudates by increased activity of stress-related mitogen-activated protein kinases.
A delicate balance is required for plants to distinguish mutualist from parasitic organisms. Mitogen-activated protein kinase (MAPK) cascade is one of the signaling modules that mediate transduction of extracellular microbial signals into appropriate cellular response. Here we employ a transgenic sy...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-07-14 | PXD006328 | Pride
Arabidopsis thaliana Proteomics Roots Metabolomics Root exudates Plant defense Metabolite profiling Signaling 
MetabolomicsStress-related mitogen-activated protein kinases stimulate the accumulation of small molecules and proteins in Arabidopsis thaliana root exudates
A delicate balance in cellular signaling is required for plants to distinguish mutualist from parasitic organisms in its environment. Mitogen-activated protein kinase (MAPK) cascades are one of the signaling modules that mediate transduction of extracellular microbial signals into appropriate cellul...
2017-08-25 | MTBLS441 | MetaboLights
ProteomicsChanges in Protein Turn-over Rates Regulate Tryptophan and Glucosinolate Biosynthesis, IAA Transport and Photosynthesis in Arabidopsis Growth and Defense Transitions
Changes in protein synthesis and degradation rates, transcript, protein and phytohormone abundance of 99 targets using qPCR and LC-MS parallel reaction monitoring (PRM) were measured in the transitions between optimal growth conditions (homeostasis), fully induced pattern triggered immunity (PTI) an...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-10-13 | PXD041215 | Pride
Translational regulation Photosynthesis Protein degradation rates Pti Glucosinolates Myc2 Phytohormone Proteomics Protein synthesis rates Auxin Pin proteins 
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