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ProteomicsAPEX2 mediated proximity labeling to investigate the proximal proteome of mitochondria tethered ULK1 in wild type and fip200 knock down cells
The object of this project is to investigate the proximal proteome of ULK1 during mitophagy and the effects of a FIP200 knock down using the APEX2-mediated proximity labeling system. APEX2-ULK1 or APEX2 was fused to 2xFKBP-myc and stably expressed in a cell line stably expressing a truncated FIS1-FR...
ORGANISM(S): Homo sapiens (Human) 
2024-10-12 | PXD047277 | Pride
Fip200 Mitophagy Autophagy Frb-rapalog-fkbp interaction Ulk1 Apex2 Er 
ProteomicsEndosulfines Igo1 and Igo2 promote hyperosmotic stress signaling via inhibition of protein phosphatase 2A
The hyperosmotic stress response in budding yeast is a paradigm for cellular responses to physicochemical stimuli that is often used for modeling signal transduction pathways. Here, we describe the phosphatase PP2A-Cdc55 as a novel master regulator of hyperosmotic stress signaling. We show that its ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-11-02 | PXD019646 | Pride
Stress response Phosphorylation Igo2 Gis1 Igo1 Phosphatase Pp2a Saccharomyces cerevisiae Quantitative proteomics Rph1 Hyperosmotic stress Stress signaling Cdc55 Endosulfines 
ProteomicsNovel interconnections of HOG signaling revealed by combined use of two proteomic software packages
Modern quantitative mass spectrometry (MS)-based proteomics enables researchers to unravel signaling networks by monitoring proteome-wide cellular responses to different stimuli. MS-based analysis of signaling systems usually requires an integration of multiple quantitative MS experiments, which rem...
ORGANISM(S): Saccharomyces Cerevisiae 
2019-06-23 | PXD013789 | panorama
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