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ProteomicsDiscovery of Tamoxifen and N-Desmethyl Tamoxifen Protein Targets in MCF-7 Cells Using Large-Scale Protein Folding and Stability Measurements
The proteins in an MCF-7 cell line were probed for tamoxifen (TAM) and n-desmethyl tamoxifen (NDT) induced stability changes using the Stability of Proteins from Rates of Oxidation (SPROX) technique in combination with two different quantitative proteomics strategies. Together the SILAC- and iTRAQ-S...
ORGANISM(S): Homo sapiens (Human) 
2019-11-08 | PXD006732 | Pride
Silac Tamoxifen Sprox Mcf-7 N-desmethyl tamoxifen 
ProteomicsDynamics of protein expression reveals primary targets and secondary messengers of estrogen receptor signaling in MCF-7 breast cancer cells
Estrogen receptor-mediated proliferation of breast cancer cells is facilitated through expression of multiple primary target genes, products of which induce the secondary response to stimulation. To differentiate between the primary and secondary target genes of estrogen receptor signaling, we measu...
ORGANISM(S): Homo sapiens (Human) 
2016-04-14 | PXD002797 | Pride
Estradiol Srm Estrogen receptor Ere Silac Estrogen-regulated proteins Mcf-7 
ProteomicsLarge-Scale Analysis of Breast Cancer-Related Conformational Changes in Proteins using SILAC-SPROX
Proteomic methods for disease state characterization and biomarker discovery have traditionally utilized quantitative mass spectrometry methods to identify proteins with altered expression levels in disease states. Here we report on the large-scale use of protein folding stability measurements to ch...
ORGANISM(S): Homo sapiens (Human) 
2017-07-10 | PXD006703 | Pride
Mcf-7 Chemical denaturation Bt-474 Mda-mb-468 Protein folding 
ProteomicsMCF-7 phosphoproteome LC-MS/MS
MCF-7 cells were stimulated with TNF-alpha in order to identify IKKb substrates. conditions: TNF alpha stimulation TNF alpha stimulation + SC-514 IKK (kinase dead mutant) + TNF alpha stimulation IKK(WT) + TNF alpha stimulation basal Already validated IKK substrates were used to train random fores...
ORGANISM(S): Homo sapiens (Human) 
2018-10-17 | PXD001873 | Pride
Mcf-7 Phosphoproteome Tnf stimulation Ikkb 
ProteomicsGlobal Analysis of Protein Folding Thermodynamics for Disease State Characterization, MCF7 vs MCF10A
Protein biomarkers can be used to characterize and diagnose disease states such as cancer. They can also serve as therapeutic targets. Current methods for protein biomarker discovery, which generally rely on the large-scale analysis of gene and/or protein expression levels, fail to detect protein b...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080760 | MassIVE
ProteomicsFunction and relevance of sushi domain-containing protein 2 (SUSD2) in circulating tumor cells of metastatic estrogen receptor positive breast cancer
Albeit responding well to therapy, estrogen receptor positive breast cancers frequently become subsequently resistant. This leads to distant metastasis and release of circulating tumor cells (CTCs) into the blood stream and ends with the death of the patient. Here we analyzed the proteome of CTC-ITB...
ORGANISM(S): Homo sapiens (Human) 
2025-02-04 | PXD047444 | Pride
Breast cancer Susd2 Estrogen receptor positive Circulating tumor cells Metastasis 
ProteomicsGlobal Analysis of Protein Folding Thermodynamics for Disease State Characterization, MCF7 vs MDAMB231
Protein biomarkers can be used to characterize and diagnose disease states such as cancer. They can also serve as therapeutic targets. Current methods for protein biomarker discovery, which generally rely on the large-scale analysis of gene and/or protein expression levels, fail to detect protein b...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080761 | MassIVE
ProteomicsLarge-Scale Analysis of Breast Cancer-Related Conformational Changes in Proteins using Limited Proteolysis
Conformational changes in proteins can lead to disease. Thus, methods for identifying conformational changes in proteins can further improve our understanding and facilitate detection of disease states. Here we combine limited proteolysis (LiP) with Stable Isotope Labeling with Amino Acids in Cell ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080770 | MassIVE
ProteomicsDiscovery of Tamoxifen and N-Desmethyl Tamoxifen Protein Targets in MCF-7 Cells Using Large-Scale Protein Folding and Stability Measurements, part 2
The proteins in an MCF-7 cell line were probed for tamoxifen (TAM) and n-desmethyl tamoxifen (NDT) induced stability changes using the Stability of Proteins from Rates of Oxidation (SPROX) technique in combination with two different quantitative proteomics strategies. Together the SILAC- and iTRAQ-S...
ORGANISM(S): Homo sapiens (Human) 
2019-11-08 | PXD006747 | Pride
Tamoxifen Sprox N-desmethyl tamoxifen 
ProteomicsHistone Kla is regulated by cellular glycolysis
We have identified a new histone modification, which is derived from glycolysis end product, lactate. By SILAC-MS/MS based quantification, and U-13C6 glucose labeling experiments, we demonstrate that histone Kla is regulated by cellular glycolysis pathway
ORGANISM(S): Homo sapiens (Human) 
2019-08-01 | PXD014870 | Pride
Human mcf-7 cells silac 
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