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ProteomicsQualitatively Different T Cell Phenotypic Responses to IL-2 versus IL-15 are Unified by Identical Dependences on Receptor Signal Strength and Duration
3 biological replicates of F15R-Kit cells stimulated with 500pM IL-2 or IL-15 for 15 minutes, and two biological replicates of unstimulated cells (total 8 conditions) were iTRAQ8plex labeled, combined and analyzed together. Phosphotyrosine peptides were enriched for by immunoprecipitation followed b...
ORGANISM(S): Homo sapiens (Human) 
2013-11-07 | PXD000440 | Pride
Phosphotyrosine Quantification Itraq8plex Il-2 Il-15 Phosphorylation 
ProteomicsQuantitative proteomic analysis of tumour tissues isolated from early and late stage human cervical cancer patients
Human papillomavirus (HPV) infection related malignancy remains a severe public health problem worldwide, although HPV prophylactic vaccine has been introduced 15 years ago . HPV-associated cancers comprise 29.1% of all 2.2 million infection-related cancers, including nearly 100% of cervical cancers...
ORGANISM(S): Homo sapiens (Human) 
2023-01-26 | PXD029103 | Pride
Itraq8plex Cervical cancer Lc-ms/ms 
ProteomicsAnalysis of pummelo proteome using iTRAQ
For protein identification, the following parameters were used. Peptide mass tolerance = 20 ppm, Missed cleavage = 2, MS/MS tolerance = 0.1 Da, Enzyme = Trypsin, Fixed modification: Carbamidomethyl (C), iTRAQ8plex(K), iTRAQ8plex(N-term), Variable modification：Oxidation(M)，Decoy database pattern=Reve...
ORGANISM(S): Citrus maxima (Pomelo) (Citrus grandis) 
2020-01-27 | PXD000527 | Pride
Itraq floral bud 
ProteomicsTemperature-dependent Proteome Study of Thermoanaerobacter tengcongensis
Comprehensive and quantitative information of the thermophile proteome is an important source for understanding of the survival mechanism under high growth temperature. Thermoanaerobacter tengcongensis (T. tengcongensis), a typical anaerobic thermophilic eubacterium, was selected to quantitatively e...
ORGANISM(S): Thermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) 
2013-08-13 | PXD000264 | Pride
Ms/ms Tte Itraq 
ProteomicsThe comparative proteomics research of Toxoplasma infection in mice liver
The comparative proteomics research of Toxoplasma infection in mice liver
ORGANISM(S): Mus musculus (Mouse) 
2016-03-31 | PXD003399 | Pride
Itraq8plex Liver Tripletof 5600 Toxoplasma gondii 
ProteomicsProtein Expression Profile of PBMC from CA and OP patient samples
To identify and quantify protein profiles from peripheral blood mononuclear cells (PBMC) of osteopetrosis patients with isobaric Tagging for Relative and Absolute protein Quantification (iTRAQ) based proteomic technology and to find differentially expressed proteins in osteopetrosis. Total protein w...
ORGANISM(S): Homo sapiens (Human) 
2013-10-11 | PXD000346 | Pride
Human  pbmc 
ProteomicsA multi-omic analysis of an Enterococcus faecium mutant reveals genetic mutation and dramatic changes of mRNA and protein expressions
Three iTRAQ8plex experiment replicate using TripleToF 5600 system. Protein identification were performed using the ProteinPilot software (version 4.0.8085,Paragon Algorithm version 4.0.0.0).All MS/MS spectra was search against amino acids sequences which is annotated from genome. The search p...
ORGANISM(S): Enterococcus faecalis (Streptococcus faecalis) 
2014-04-16 | PXD000326 | Pride
Enterococcus faecium Lc-ms/ms Transcript Space flight Protein 
ProteomicsDifferentially embedded glioblastoma tumor protein quantitation
Glioblastoma multiforme (GBM) is the most aggressive malignant primary brain tumor with a dismal mean survival even with the current standard of care. Although in vitro cell systems can provide mechanistic insight into the regulatory networks governing GBM cell proliferation and migration, clinical ...
ORGANISM(S): Homo sapiens (Human) 
2014-07-09 | PXD001038 | Pride
Glioblastoma Quantitation Protein expression Itraq Tumor 
ProteomicsSemen of gibel carp - Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish
In our study, differential male nucleus events and development behaviors were revealed from the fertilized eggs in response to the sperm from males of genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) in gibel carp. When the eggs of maternal fish were fertilized by ...
ORGANISM(S): Carassius gibelio 
2018-06-14 | PXD008919 | Pride
Gibel carp Semen proteomics Gsd Tsd Itraq 
ProteomicsThinPrep Cervical Smear iTRAQ LC-MS/MS
The protein content from ThinPrep cervical smear specimens was purified with TCA precipitation. The protein pellets were dissolved and protein concentration was measured with the Bradford assay. 100 ug of protein for each sample were reduced with TCEP, cysteines were blocked with MMTS and finally d...
ORGANISM(S): Homo sapiens (Human) 
2013-05-09 | PXD000109 | Pride
Hpv Orbitrap elite Thinprep Itraq 
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