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ProteomicsProteomics from centrosome-enriched fractions of Jurkat E6-1 human T cells, human embryonic kidney 293 cells, resting human T lymphoblasts and activated human T lymphoblasts
Centrosome-enriched fractions of Jurkat E6-1 human T cells (JK), human embryonic kidney (HEK) 293 cells, resting human T lymphoblasts (LT CTRL) and activated human T lymphoblasts (LT ACT), with one biological replicate for each condition, were analysed by label-free LC-MS/MS. For further details, re...
ORGANISM(S): Homo sapiens (Human) 
2025-12-18 | PXD063199 | Pride
Hek T lymphoblasts Jurkat Post-translational modifications Human Itraq Lc-ms/ms 
ProteomicsTime-course of ischemia-reperfusion in a pig model
We have performed an unbiased, open-search analysis of the oxidative PTMs and quantitative multiplexed proteomics that take place in the pig ischemic heart tissue during the first 24h after I/R. We used 40 castrated male Large-White pigs weighing 30 to 40 kg. Closed-chest 40 min I/R was performed i...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2024-05-24 | PXD045771 | Pride
Ptms Ischemic heart tissue Ischemia reperfusion (i/r) Quantitative proteomics Pig 
ProteomicsTime-course of ischemia-reperfusion in a mouse model
We have performed an unbiased, open-search analysis of the oxidative PTMs and quantitative multiplexed proteomics that take place in the mouse heart tissue during the first 48h after I/R. Surgically-induced MI was performed and animals were sacrificed at 10 min, 20 min, 40 min, 80 min, 2 hours, 6 ho...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-24 | PXD045759 | Pride
Ptms Mouse Ischemia reperfusion (i/r) Heart tissue Quantitative proteomics 
ProteomicsProteomics analysis of myocardial tissue from ischemia/reperfusion (I/R) pig model treated with cardiosphere-derived cells (CDCs)
Some studies report better outcomes in cell therapy for myocardial infarction (MI) with repeated administrations. We aimed to elucidate the potential differences in terms protein expression after one or three doses of cardiosphere-derived cells (CDCs) in a porcine MI model. CDCs were isolated from s...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2025-12-22 | PXD070301 | Pride
Quantitative proteomics I/r pig model Tmt18plex Cardiosphere-derived cells 
ProteomicsMultiplexed quantitative proteomics of extracellular vesicles released by endMSCs (EV-endMSCs)
The extracellular vesicles released by endMSCs (EV-endMSCs)were characterized by multiplexedquantitative proteomics approach. We hypothesized that inflammatory priming of adult stem cells may contribute to increase their therapeutic effectiveness, thus, extracellular vesicles derived from IFN-γ-prim...
ORGANISM(S): Homo sapiens (Human) 
2019-12-17 | PXD015465 | Pride
Ifnγ-primed Extracellular vesicles Human mesenchymal stem cells Proteomic Endometrial 
ProteomicsNeutrophil depletion experiment in ischemia reperfusion mouse model
To elucidate the role of neutrophils in the molecular alterations observed after I/R, we performed an experiment of I/R in neutrophil-depleted mice. Neutrophil depletion was performed by anti-Ly6G injection and IgG was used as vehicle. We have performed an unbiased, open-search analysis of the oxida...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-24 | PXD045756 | Pride
Ischemia reperfusion Mouse Heart tissue Quantitative proteomics 
ProteomicsProteomics from centrosome-enriched fractions from resting and activated T lymphoblasts
Human resting and activated T lymphoblast (LT CTRL and LT ACT) centrosome-enriched subcellular fractions from LT CTRL and LT ACT were prepared as analysed by multiplexed quantitative proteomics and LC-MS/MS. Samples from different donors were pooled after first lysis. For further details, refer to r...
ORGANISM(S): Homo sapiens (Human) 
2025-12-18 | PXD063195 | Pride
T lymphoblasts Post-translational modifications Human Itraq Lc-ms/ms 
ProteomicsIdentification of spidroin proteins in the silk glands of Nephila clavipes spiders
Glands were frozen at -80 ºC immediately after being retrieved and stored under this condition until initiating the proteomics analysis protocol. Glands were washed with cold PBS and immersed in 120 l of homogenization buffer (50 mM Tris-HCl, pH 6.8, 10 mM DTT, 4% (w/v) SDS). Glands were boiled for ...
ORGANISM(S): Trichonephila clavipes 
2022-12-07 | PXD023141 | Pride
Spider silk  proteomics  liquid chromatography-tandem mass spectrometry  silk gut 
MetabolomicsFlow cytometry has a significant impact on the cellular metabolome (Lipidomic LC-MS assay)
The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this ...
2018-11-06 | MTBLS631 | MetaboLights
ProteomicsCharacterization of HEK293 secretome by LC-MS/MS
Characterization of the extracellular environment of produced VLPs and coproduced extracellular vesicles (EVs)by quantitative proteomics approach. Three conditions were studied: non-transfected, transfected with an empty plasmid as control and with a plasmid coding for HIV-1 Gag polyprotein.
ORGANISM(S): Homo sapiens (Human) 
2020-09-28 | PXD018114 | Pride
Separation downstream processes Microvesicles Vlp Mass spectrometry Hiv Immunomodulation Exosomes 
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