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ProteomicsShotgun analysis of nuclear proteins from mouse brain
Nuclei were isolated from the mouse brain. Proteins were extracted with addition of Bensonase and cleaned up by acetone precipitation. Proteis were resuspended in 50 mM ammonium bicarbonate and PPS Silent Surfactant. Samples were sonicated on ice to improve the solubility of proteins, then alkylated...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
MSV000079301 | MassIVE
ProteomicsProteomic and bioinformatic analysis of the nuclear intrinsically disordered proteome
Intrinsically disordered proteins (IDPs) are biologically active molecules which are involved in many cellular functions although they do not possess a defined three-dimensional structure. They are mostly signalling and regulatory proteins. This study is the first large-scale proteomic analysis of t...
ORGANISM(S): Homo sapiens (Human) 
2015-09-21 | PXD002676 | Pride
Nuclear proteome Subproteome Transcription factors Intrinsically disordered proteins 
ProteomicsIs transthyretin a regulator of Ubc9 SUMOylation?
Ageing and mutations of transthyretin (TTR), the thyroid hormones and retinol transporting protein lead to amyloidosis by destabilizing the structure of TTR. Because protein structure is regulated through posttranslational modifications, we investigated the Small Ubiquitin-like Modifier (SUMO)ylatio...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080754 | MassIVE
ProteomicsImipramine affects the expression of proteins involved in oxidative stress and immunity in rat primary glial cell culture
Glial cells (microglia and astrocytes) have recently became appreciated as an important target for antidepressant drugs. Here we report on the results of comprehensive proteomic analysis of the alteration in protein profile of rat primary mixed glial culture exposed to imipramine. Two-dimensional di...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-05-17 | PXD007977 | Pride
Imipramine  mixed glial cell culture  microglia  proteome  2d-dige 
ProteomicsAn insight into the proteome of uveal melanoma-derived ectosomes reveals the presence of potentially useful biomarkers
Cancer cells are known to release extracellular vesicles that often promote disease development and progression. The present study investigated protein content and glycosylation pattern of ectosomes released in vitro by human primary uveal melanoma Mel202 cell line. Ectosomes released by Mel202 cell...
ORGANISM(S): Homo sapiens (Human) 
2019-08-08 | PXD012003 | Pride
Glycosylation Uveal melanoma Proteomics Extracellular vesicles Lectins Ectosomes 
ProteomicsIntegrative genomics reveal a role for MCPIP1 in adipogenesis and adipocyte metabolism
Obesity is considered a serious chronic disease, which is associated with increased risk of developing cardiovascular diseases, non-alcoholic fatty liver disease and type 2 diabetes. Monocyte chemoattractant protein-1-induced protein-1 (MCPIP1), also called Regnase-1, is an RNase that decreases stab...
ORGANISM(S): Mus musculus (Mouse) 
2020-01-07 | PXD010148 | Pride
Adipogenesis Adipocytes Mcpip1 Lc-ms/ms 
ProteomicsProteome analysis of PC12 cells reveals alterations in translation regulation and actin signalling induced by clozapine
Although antipsychotics are routinely used in the treatment of schizophrenia for last decades, their precise mechanism of action is still unclear. In this study we investigated changes in PC12 cells’ proteome under the influence of clozapine, risperidone and haloperidol to identify protein pathways ...
ORGANISM(S): Rattus norvegicus (Rat) 
2021-09-08 | PXD014422 | Pride
Proteome Clozapine Translation Actin signalling Mtor 
ProteomicsIs transthyretin a regulator of Ubc9 SUMOylation?
Ageing and mutations of transthyretin (TTR), the thyroid hormones and retinol transporting protein lead to amyloidosis by destabilizing the structure of TTR. Because protein structure is regulated through posttranslational modifications, we investigated the Small Ubiquitin-like Modifier (SUMO)ylatio...
ORGANISM(S): Homo sapiens (Human) 
2016-08-15 | PXD004515 | Pride
Post-translational modifications Sumoylation Transthyretin Ubc9 Ufds 
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