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ProteomicsAPEX2 mediated proximity labeling to investigate the proximal proteome of mitochondria tethered ULK1 in wild type and fip200 knock down cells
The object of this project is to investigate the proximal proteome of ULK1 during mitophagy and the effects of a FIP200 knock down using the APEX2-mediated proximity labeling system. APEX2-ULK1 or APEX2 was fused to 2xFKBP-myc and stably expressed in a cell line stably expressing a truncated FIS1-FR...
ORGANISM(S): Homo sapiens (Human) 
2024-10-12 | PXD047277 | Pride
Fip200 Mitophagy Autophagy Frb-rapalog-fkbp interaction Ulk1 Apex2 Er 
ProteomicsIP-MS of human YKT6 upon mutation of possible ULK1-phosphorylation sites
Autophagy is a catabolic process during which cytosolic material is enwrapped in a newly formed double membrane structure called the autophagosome, and subsequently targeted for degradation in the lytic compartment of the cell. The fusion of autophagosomes with the lytic compartment is a tightly reg...
ORGANISM(S): Homo sapiens (Human) 
2023-03-02 | PXD038148 | Pride
Human Q exactive hf Hela Ip-ms Ykt6 Lfq 
ProteomicsPhosphorylation Mapping of Atg13 in Response to Atg1 Activity and Rapamycin Treatment in Budding Yeast
We performed phosphorylation site mapping on of budding yeast Atg13 applying a quantitative mass spectrometry-based proteomics approach based on stable isotope labeling with amino acids in cell culture (SILAC). We determined changes in the Atg13 phosphorylation pattern in response of inhibition of k...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-01-20 | PXD028461 | Pride
Phosphorylation Autophagy Saccharomyces cerevisiae Quantitative proteomics 
ProteomicsProximity labelling identification of ULK1 wild-type (WT) and ULK1-K46I (kinase dead) interactors upon mitochondria tethering in U2OS WT cells
This project involved generating three stable cell lines using a Flp-In system, each containing specific integrated constructs: "FKBPx2-myc-APEX2-ULK1", "FKBPx2-myc-APEX2-ULK1-K46I" (kinase-dead mutant), and "FKBPx2-myc-APEX2" (control). All cell lines additionally express an outer mitochondrial mem...
ORGANISM(S): Homo sapiens (Human) 
2026-04-24 | PXD068381 | Pride
Mitophagy Autophagy Ulk1-k46i Ulk1 Proximity labeling Apex2 
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