Sort   by:  
 Page size 
ProteomicsQuantitation of plasma protein concentrations by mass spectrometry without the use of isotopic standards. C4PR_LIV
We applied a non targeted mass spectrometric assay (LCMSE), to quantify 55 abundant plasma proteins in specimens from 31 healthy volunteers. Quantitation was done by HI3 peptide measurement using a single internal standard to estimate protein concentrations. Results for 7 apolipoproteins were compar...
ORGANISM(S): Homo sapiens (Human) 
2015-10-20 | PXD000347 | Pride
Lcmse Quantitation Mass spectrometry Plasma proteins Proteomics Immunoassay 
ProteomicsMolecular physiological characterization of a high heat resistant spore forming Bacillus subtilis food isolate
Bacterial endospores (spores) are among the most resistant living forms on earth. Spores of Bacillus subtilis A163 show extremely high resistance to wet heat compared to spores of laboratory strains. In this study, we found that spores of B. subtilis A163 were indeed very wet heat resistant and rele...
ORGANISM(S): Bacillus Subtilis 
2021-03-21 | MSV000087080 | MassIVE
ProteomicsHigh resolution time-lapse proteomic analysis of sporulation
Bacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome changeover during sporulation, a time-lapse proteomic analysis was performed in a cell population that was induced to sporulate synchronously. Here, we are the first to comprehensively inve...
ORGANISM(S): Bacillus Subtilis 
2021-04-03 | MSV000087154 | MassIVE
ProteomicsYBX3 is a versatile RNA-binding protein that controls amino acid import by regulating SLC mRNA abundance
Summary: Amino acids (AA) support protein synthesis, and thus cell growth and proliferation. Specialized transporters mediate AA exchange across membranes and their regulation is critical for AA homeostasis. Here, we report that the DNA- and RNA-binding protein YBX3 regulates the expression of AA t...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-11-06 | MSV000083108 | MassIVE
ProteomicsLabel-free LC-MSe in tissue and serum reveals protein networks underlying differences between benign and malignant serous ovarian tumors
Purpose: To identify proteins and (molecular/biological) pathways associated with differences between benign and malignant epithelial ovarian tumors. Experimental procedures: Serum of six patients with a serous adenocarcinoma of the ovary was collected before treatment, with a control group consisti...
ORGANISM(S): Homo sapiens (Human) 
2014-10-13 | PXD001084 | Pride
Ovarian cancer Topx peptide quantitation Serum Tissue All ions fragmentation Label free quantitation 
ProteomicsFunctional characteristics of neonatal rat beta cells With distinct markers
Neonatal beta cells are considered developmentally immature and hence less glucose-responsive. To study the acquisition of mature glucose-responsiveness, we compared glucose-regulated redox state, insulin synthesis and secretion of beta cells purified from neonatal or 10-weeks old rats to their tran...
ORGANISM(S): Rattus norvegicus (Rat) 
2014-12-12 | PXD001577 | Pride
Lcmse Rat Diabetes Label free quantitation Pancreas 
ProteomicsQuantitative proteomics of human pancreatic beta cells
Outline: using label-free alternate-scanning LC-MS/MS, we compared the proteomic architecture of core functional pathways in pancreatic beta cells of human origin. Results: we quantified the relative molar abundance of 707 proteins mainly composed of functional pathways of metabolism, protein synthe...
ORGANISM(S): Homo sapiens (Human) 
2015-08-07 | PXD001539 | Pride
Human Lcmse Diabetes Biomarker Proteomics Islet beta cell Pancreas 
ProteomicsElevated gpNMB in type 1 Gaucher disease patients
Gaucher disease (GD) is characterized by the presence of glucosylceramide-laden macrophages (Gaucher cells) as the result of deficiency in the lysosomal hydrolase glucocerebrosidase (GBA). Non-neuronopathic type 1 GD is effectively treated by infusions with macrophage-targeted recombinant glucocereb...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD001598 | Pride
Gpnmb Lcmse Gaucher disease Proteomics Biomarker 
ProteomicsNeisseria meningitides small RNA's regulate Citric acid cycle activity under Feast and Famine conditions
We identified twin small non-coding RNAs regulating tricarboxylic acid (TCA) cycle activity in Neisseria meningitidis. Expression of TCA cycle enzymes was elevated in sRNA deletion mutants. Direct interaction between sRNAs and the ribosomal entry sites on target mRNAs was demonstrated. Expression of...
ORGANISM(S): Neisseria meningitidis serogroup B 
2017-03-22 | PXD000891 | Pride
Data independant mass spectrometry Pathogens Neisseria meningitides Feast and famine Small rna's 
ProteomicsDynamics of the physiology, metabolome and proteins during the circadian cycle of Synechocystis sp. PCC 6803 under culturing conditions relevant for mass culturing
Here, we present the results of a study of the model cyanobacterium Synechocystis sp. PCC 6803, cultured in a lab-scale photobioreactor simulating physiological conditions present during large-scale culturing. The culture is flushed with N2 and CO2 without additional O2. This leads to an anoxic envi...
ORGANISM(S): Synechocystis sp. PCC 6803 
2016-09-27 | PXD001619 | Pride
Lcmse Synechocystis sp. pcc 6803 Proteomics 
Sort   by:  
 Page size