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ProteomicsProtein co-immmunoprecipitation using antibodies specific for the antimicrobial peptide FsPDF2 from Fagus sylvatica
To assess the specificity of the antibodies used in the quantitative sandwich ELISA, co-immunoprecipitation (CoIP) was performed. Proteins binding to these antibodies were detected using LC-MS/MS in DDA mode.
ORGANISM(S): Fagus sylvatica 
2026-06-08 | PXD060631 | Pride
Co- immunoprecipitation (coip) Antimicrobial peptides Fagus sylvatica 
ProteomicsMS-based identification of small proteins in Campylobacter jejuni
The Gram-negative bacterium Campylobacter jejuni is a widespread food-borne pathogen. Knowing the proteins encoded by the bacterial pathogen and how their expression is regulated is essential to understand how it survives, colonizes, and causes diseases. The present study focusses on small proteins...
ORGANISM(S): Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 
2024-05-23 | PXD036790 | Pride
Ribosome profiling Gelfree fractionator Sorf Small proteins Gelc-ms/ms Campylobacter jejuni Translatome 
ProteomicsMembrane proteins of Planctopirus limnophila
Bacteria of the phylum Planctomycetes have been postulated to be the ‘missing link’ between prokaryotes and eukaryotes. Although phylogenetically the Planctomycetes were grouped into Gram-negative bacteria, it has been reported that they have no outer membrane. Here we present 1D-gel-LC/MS/MS based...
ORGANISM(S): Planctopirus limnophila DSM 3776 
2017-04-18 | PXD005738 | Pride
Planctomycetes 1d-gel-lc-ms/ms Membrane proteins 
ProteomicsAdaptation of Dinoroseobacter shibae to different kinds of oxidative stress
The Gram-negative photoheterotrophic bacterium Dinoroseobacter shibae is a member of the high abundant marine Roseobacter group. Living in the photic zone environment of marine ecosystems D. shibae is frequently exposed to oxygen. Oxic environments are hazardous and therefore effective defense mecha...
ORGANISM(S): Dinoroseobacter shibae (strain DFL 12) 
2021-03-12 | PXD013791 | Pride
Diamide Paraquat Dinoroseobacter shibae Oxidative stress Hydrogen peroxide Gelc-ms/ms Rhizobial iron regulator 
ProteomicsWithin-host adaptive phenotypic responses of bovine Staphylococcus aureus
The Gram-positive bacterium S. aureus is one of the most common causes of contagious bovine mastitis. A predominant S. aureus clone persists within its host over a long period. In the present study, we compared the surface proteome of two isolates, collected from a naturally infected dairy cow with ...
ORGANISM(S): Staphylococcus aureus 
2021-08-14 | PXD025013 | Pride
Chronic bovine mastitis Surface shaving Biotinylaton Host adaption Staphylococcus aureus Cell surface proteome 
ProteomicsIdentification and quantification of small proteins and peptides in Staphylococcus aureus
Very small proteins with less than 100 amino acids (SP100) of Staphylococcus aureuse are underrepresented in proteomic analyses so far. However, in the last few years a variety of such small proteins with regulatory and virulence associated functions have been detected in several bacteria. The intro...
ORGANISM(S): Staphylococcus aureus 
2021-05-26 | PXD017932 | Pride
Proteogenomics Sp100 Small proteins Staphylococcus aureus 
ProteomicsIdentification of small proteins in nodules of Robinia pseudoacacia
Robinia pseudoacacia L. (black locust) is capable of nitrogen fixation in association with rhizobia. Global transcriptional analysis in Robinia pseudoacacia nodules revealed the presence of three highly abundant small transcripts that may encode small proteins. The high number of proline and glycin...
ORGANISM(S): Robinia pseudoacacia 
2026-03-06 | PXD044747 | Pride
ProteomicsBiogenesis and DNA cargo of Dinoroseobacter shibae outer membrane vesicles suggests vesicle formation is coupled to replication
The Gram-negative photoheterotrophic bacterium Dinoroseobacter shibae is a member of the high abundant marine Roseobacter group. In the ocean, OMVs have about the same abundance as bacteria, a distinct depth distribution, and contain DNA from a variety of marine bacterial taxa. In the present study,...
ORGANISM(S): Dinoroseobacter shibae (strain DFL 12) 
2020-12-16 | PXD014351 | Pride
Dinoroseobacter shibae Gelc-ms/ms Outer membrane vesicles (omvs) 
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