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MetabolomicsGain-of-function genetic screens in human cells identify SLC transporters overcoming environmental nutrient restrictions

Solute carrier (SLC) transporters control fluxes of nutrients and metabolites across membranes and thereby represent a critical interface between the microenvironment and cellular and subcellular metabolism. Because of substantial functional overlap, the interplay and relative contributions of SL...

2022-09-20 | MTBLS4268 | MetaboLights
MetabolomicsSystematic analyses of lipid mobilization by human lipid transfer proteins

Lipid transfer proteins (LTPs) maintain the specialised lipid compositions of biological membranes, and many are associated with disease. In eukaryotes, they support organellar functions by transporting lipids between compartmentalised metabolic pathways. However, for the majority of the hundreds...

2025-11-14 | MTBLS9567 | MetaboLights
ProteomicsImproved Sensitivity in Low-Input Proteomics using Micro-Pillar Array-based Chromatography
Capitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nano-LC-ESI-MS/MS is currently the most sensitive analytical technology for the comprehensive characterization of complex protein samples. However, despite tremendous technological impr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014124 | Pride
Nano-lc-esi-ms/ms nano-flow hplc hela cell digest 
ProteomicsBoosting MS1-only proteomics with machine learning allows 2000 protein identifications in single-shot human proteome analyses using 5-minute HPLC gradients
Proteome-wide analyses rely on tandem mass spectrometry and extensive separation of proteolytic mixtures imposing considerable instrumental time consumption that is one of the main obstacles in a broader acceptance of proteomics in biomedical and clinical research. Recently, we presented a fast prot...
ORGANISM(S): Homo sapiens (Human) 
2021-03-18 | PXD023977 | Pride
Faims Fusion lumos Mass spectrometry Ms1-only Hela Protein identification 
ProteomicsRetention time calibration for RP-HPLC at pH 9
The change in pH of the mobile phase drastically changes the retention mechanism of peptides, rendering currently available retention time prediction models useless. The aim of this study is to create a dataset of peptide retention times under basic (pH 9) HPLC conditions, allowing for the creation ...
ORGANISM(S): Homo sapiens (Human) 
2026-02-13 | PXD064311 | Pride
Hela Basic ph Rp-hplc 
ProteomicsDevelopment and performance evaluation of an ultra-low flow nano liquid chromatography-tandem mass spectrometry set-up
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the most commonly used technique for the identification and characterization of proteins. The ionization and transmission efficiency of the electrospray process is a critical factor in LC-MS/MS, ultimately limiting the sensitivity of the a...
ORGANISM(S): Homo sapiens (Human) 
2014-07-10 | PXD000396 | Pride
Lc-ms hplc gradient human hela 
ProteomicsA PrSM-level Side-by-Side Comparison of On-line LCMS methods on Intact Histone H3 and H4 Proteoforms
The heterogeneity of histone H3 proteoforms has made histone H3 top-down analysis challenging. To enhance the detection coverage on the proteoforms, performing liquid chromatography (LC) front-end to MS detection is recommended. Here, by using optimized Electron-Transfer/High-Energy Collision Dissoc...
ORGANISM(S): Homo sapiens (Human) 
2025-03-03 | PXD026820 | Pride
Top-down proteomics Histones Post-translation modifications On-line lcms Rplc Esi-compatible wcx/hilic 
ProteomicsGlobal and diGLY enriched proteomes of RanBPM depleted HeLa cells and RanBPM APMS
The human CTLH complex is a newly discovered multi-subunit E3 ligase. At present, only a few of its ubiquitination targets are known. Here, we use proteomic techniques in RanBPM-depleted HeLa cells to identify ubiquitination substrates of the CTLH complex. First, global proteomics determined protein...
ORGANISM(S): Homo sapiens (Human) 
2021-08-13 | PXD024872 | Pride
Human Hela Ctlh complex 
ProteomicsFrom HeLa to human blood: extending single-cell proteomics to the smallest immune cells – REVISION
Single-cell proteomics (SCP) holds the promise of decoding cellular heterogeneity at the functional level, yet achieving deep and reproducible proteome coverage from individual cells has remained a formidable challenge. Here, we establish a high-sensitivity, label-free SCP platform that extends curr...
ORGANISM(S): Homo sapiens (Human) 
2026-08-20 | PXD078497 | Pride
Single-cell proteomics Orbitrap astral zoom Pbmc Hela Scp 
ProteomicsQuantitative proteomics of nutrient starved wildtype (WT) and MTM1 knock out (KO) HeLa cells.
Quantitative whole cell proteomics that compare fed or starved wildtype (WT) and MTM1 KO HeLa cells.
ORGANISM(S): Homo sapiens (Human) 
2022-12-22 | PXD033850 | Pride
Lc-ms Starvation Hela Tmt-labeling Mtm1 
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