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ProteomicsHuman glomerular extracellular matrix analysed by LC-MSMS
Extracellular matrix proteins were isolated from human glomeruli and analysed by LC-MSMS
ORGANISM(S): Homo sapiens (Human) 
2014-01-22 | PXD000456 | Pride
Human Glomerulus Kidney Extracellular matrix 
ProteomicsHuman glomerular cell-derived extracellular mantrix
We isolated the extracellular matrix from human podocytes and endothelial cells in monoculture and coculture and analysed the matrix proteins by mass-spectrometry. The glomerular basement membrane (GBM) is a specialized extracellular matrix (ECM) compartment within the glomerulus and is known to con...
ORGANISM(S): Homo sapiens (Human) 
2014-01-29 | PXD000643 | Pride
Coculture Endothelial cells Pododcytes 
ProteomicsInvestigating the effect of fluid flow on glomerular cell-derived matrix
The glomerular basement membrane has a complex composition and structure in vivo. In vitro models of the glomerular capillary wall have been developed using microfluidic and perfusion systems, however, how the extracellular matrix alters under static and flow conditions has not yet been investigate...
ORGANISM(S): Homo sapiens (Human) 
2026-02-02 | PXD044226 | Pride
Glomerular matrix Microfluidic bioreactor Glomerular filtration barrier 
ProteomicsProteomic profiling of kidney biopsies in nephrotic syndrome
Minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS) are patterns of kidney injury observed in the filtering units of the kidney known as glomeruli. From the NURTuRE cohort of individuals with nephrotic syndrome (NS), we performed laser microdissection and mass spectrometry ana...
ORGANISM(S): Homo sapiens (Human) 
2024-07-15 | PXD053362 | Pride
Nurture cohort Laser-microdissection Global proteomics Minimal change Focal segmental glomerulosclerosis Nephrotic syndrome 
ProteomicsIntegrin adhesion complexes formed on different basement membrane ligands
Adhesion complexes isolated from cells in culture. Podocytes were attached to different extracellular matrix ligands and adhesion complex isolated.
ORGANISM(S): Homo sapiens (Human) 
2020-03-09 | PXD017913 | Pride
Podocyte Ab Extracellular matrix ligand. Integrins Adhesions Rpe19 
ProteomicsHuman kidney proteomics, glomerular and tubular cellular and matrix fractions
Glomerular and tubulointerstitial fractions were enriched from human kidneys samples. Ages of human kidney samples: 15, 29, 37, 61, 67 and 69 years of age. The glomerular and tubulointerstitial samples were processed to enrich for extracellular matrix components and the fractions analysed by mass sp...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD022219 | Pride
Tubulointerstitial Tubular Glomerular Kidney Age 
ProteomicsProteomic analysis of induced pluripotent stem cell-derived kidney organoids
Kidney organoids were generated from a control iPSC line using a previously published protocol (https://doi.org/10.1038/nprot.2016.098). Organoids were collected at three timepoints during the protocol (day 14, 18 and 25) and prepared for proteomic analyses. The focus of the study was to define the ...
ORGANISM(S): Homo sapiens (Human) 
2021-10-13 | PXD025838 | Pride
Mass spectrometry Basement membrane Kidney organoid Enrichment Kidney Extracellular matrix 
ProteomicsProteomic analysis of healthy and hyperglycemic murine fetal kidneys
Alteration of kidney morphogenesis in diabetic pregnancies is poorly described, especially changes in the extracellular matrix (ECM) and glomerular basement membrane (GBM during glomerulogenesis. Addressing the ECM proteome, or matrisome, in the mouse fetal kidney in a healthy and diabetic environme...
ORGANISM(S): Mus musculus (Mouse) 
2021-10-13 | PXD025874 | Pride
Mass spectrometry Basement membrane Enrichment Kidney Extracellular matrix Diabetes 
ProteomicsMouse kidney glomerular extracellular matrix LC-MSMS
Across the spectrum of human disease there are significant changes in the composition and organisation of extracellular matrix (ECM). Furthermore, disease severity can vary according to race and sex, yet mechanisms for these variations are unclear. Using the kidney glomerulus as a model system, we t...
ORGANISM(S): Mus musculus (Mouse) 
2016-07-09 | PXD000811 | Pride
Lc-msms Mouse Kidney Glomerular Extracellular matrix 
ProteomicsCol4a1 mouse glomerular datasets
Glomeruli were isolated from Col4a1+/SVC and Ctrl mice. Samples were enriched for extracellular matrix. Glomerular extracellular matrix and cellular fractions were analysed by mass spectrometry.
ORGANISM(S): Mus musculus (Mouse) 
2022-08-12 | PXD022363 | Pride
Matrix fraction Glomerulus Mouse Kidney Col4a1 Cellular fraction 
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