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ProteomicsMapping Protein Targets of Bioactive Small Molecules using Lipid-Based Chemical Proteomics
Lipids play critical roles in cell biology, often through direct interactions with proteins. We recently described the use of photoreactive lipid probes combined with quantitative mass spectrometry to globally map lipid-protein interactions, and the effects of drugs on these interactions, in cells. ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-09-26 | PXD007570 | Pride
Hek293t Neuro2a Orbitrap velos Chemoproteomics Lipid probes 
TranscriptomicsLinalool, an aromatic terpenoid, rewires transcriptome profile of lipid-loaded HepG2 cells
The microarray results showed that linalool sitimulation of lipid-loaded HepG2 cells rewired the hepatic transcriptome profile, with linalool being comparable to those of fenofibrate. Total of 8,988 genes that were commonly and significantly expressed in all experimental groups, including control he...
ORGANISM(S): Homo sapiens 
2013-04-11 | E-GEOD-45961 | biostudies-arrayexpress
ProteomicsIdentification of proteins crosslinked to lysosomal sphingosine and cholesterol probes
HeLa cells were labelled with lysosomally localized photocrosslinkable and clickable probes for sphingosine and cholesterol. Upon photocrosslinking and cell lysis, the protein-lipid complexes were clicked to biotin azide and enriched via Streptavidin-immunoprecipitation. Enriched protein-lipid compl...
ORGANISM(S): Homo sapiens (Human) 
2023-02-07 | PXD039780 | Pride
Lysosomal lipids Photoaffinity labelling Click chemistry 
ProteomicsMapping of mycobacterial lipolytic enzymes involved in triacylglycerol accumulation as intrabacterial lipid inclusions using multi-target inhibitor-like affinity-based probes
Mycobacteria are known for their unique and complex cell wall architecture, characterized by distinct lipid-rich outer layers, making lipid metabolism a central point of their physiological processes. One crucial aspect of all mycobacteria is their ability to metabolize host lipids and to accumulate...
ORGANISM(S): Mycobacterium abscessus 
2025-05-12 | PXD048770 | Pride
Mycobacterium Lc_msms Activity-based protein profiling  multitarget inhibitor-like affinity-based probes  lipid metabolism  cyclipostins & cyclophostin  oxadiazolone 
ProteomicsAlkylation Damage by Lipid Electrophiles Targets Functional Protein Systems
These data correspond to "Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems," a paper from Codreanu, Liebler, et al, published at the journal _Molecular and Cellular Proteomics_ in 2013. Modifications also include: 4-hydroxynonenal (HNE) (UniMod #53), H(16) C(9) O(2), Mono...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2013-11-18 | MSV000078490 | MassIVE
ProteomicsTrifunctional lipid derivatives: PEs mi-tochondrial interactome
In this study, we employed chemically synthesized trifunctional lipid probes to identify putative protein partners that interact with lipids in HeLa cells. These probes incorporate three distinct chemical functionalities. First, a coumarin-based “caging” moiety is added to the lipid head group, whic...
ORGANISM(S): Homo sapiens (Human) 
2025-01-21 | PXD059960 | Pride
Phosphatidic-acid Trifunctional lipid Phospholipid Phosphatidylinositol Phosphatidylethanolamine Lipid interactome 
ProteomicsAlkylation Damage by Lipid Electrophiles-- HNE-induced expression in RKO cells
4-hydroxynonenal (HNE) (UniMod 53) (+156.115030 Da on Cys, His, Lys) reduced 4-Hydroxynonenal (UniMod 335(+158.130680 Da on Cys, His, Lys) These data were additions to the set for the paper Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems by Codreanu et al, Mol. Cell. Pro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2013-12-18 | MSV000078512 | MassIVE
ProteomicsActivity-based protein profiling in human M2 macrophages using DHA-based photoaffinity probes
17-Hydroxydocosahexaenoic acid (17-HDHA), an oxidative metabolite of the fish oil constituent docosahexaenoic acid (DHA, 22:6 n-3), is a signaling lipid with anti-inflammatory properties. The molecular mechanisms underlying the biological effect of 17-HDHA are poorly understood. Here, we report the ...
ORGANISM(S): Homo sapiens (Human) 
2023-04-28 | PXD027600 | Pride
Human Abpp Photoaffinity Macrophage 
TranscriptomicsRegulation of host weight gain and lipid metabolism by bacterial bile acid modification in the gut
Alterations in the gastrointestinal microbiota have been implicated in obesity in mice and humans, but the conserved microbial functions that influence host energy metabolism and adiposity have not been determined. Here we show that bacterial bile salt hydrolase (BSH) controls a microbe-host dialogu...
ORGANISM(S): Mus musculus 
2014-05-09 | E-GEOD-46952 | biostudies-arrayexpress
UnknownQuantification and visualization of phosphoinositides by quantum dot-labeled specific binding-domain probes.
Not available
S-EPMC3307658 | biostudies-literature
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