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ProteomicsMass spectrometry distinguishes putrescine, spermidine, and spermine covalently attached to proteins
Bacterial and mammalian cells are rich in putrescine, spermidine and spermine. Polyamines can be incorporated into proteins in vitro. Very few naturally occurring polyaminated proteins have been identified. Bovine albumin and the recombinant universal stress protein from Francisella tularensis we...
ORGANISM(S): Bos taurus (Bovine) 
2024-02-27 | PXD046396 | Pride
Polyamine Universal stress protein Transglutaminase Mass spectrometry Manual evaluation Carbodiimide Albumin 
ProteomicsEvaluation of mass spectrometry MS/MS spectra for the presence of isopeptide crosslinked peptides
Isopeptide crosslinked proteins can be the product of transglutaminase or of exposure to organophosphorus toxicants (OP). Transglutaminase links glutamine to lysine with loss of ammonia. OP toxicants induce a link between glutamic acid and lysine with loss of water. Our goal was to establish crit...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2021-07-12 | PXD026790 | Pride
Transglutaminase Dansyl cadaverine Isopeptide Dansyl-aminohexyl qqiv Protein prospector Map-rich tubulin Neurodegenerative disease Crosslinks 
ProteomicsEvaluation of mass spectrometry MS/MS spectra for the presence of isopeptide crosslinked peptides
Chronic low dose exposure to organophosphorus pesticides is associated with risk of neurodegenerative disease. The mechanism of neurotoxicity is unknown, though it is independent of acetylcholinesterase inhibition. Adducts on tyrosine, lysine, threonine, and serine can occur after exposure to organo...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-02-17 | PXD027203 | Pride
Transglutaminase Dansyl cadaverine Isopeptide Dansyl-aminohexyl qqiv Protein prospector Map-rich tubulin Neurodegenerative disease Crosslinks 
ProteomicsOrganophosphorus pesticides catalyze protein crosslinking
Exposure to organophosphorus pesticides (OP) can have chronic adverse effects that are independent of inhibition of acetylcholinesterase, the classic target for acute OP toxicity. In pure proteins, the organophosphorus pesticide chlorpyrifos oxon induces a crosslink between lysine and glutamate (or...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2022-10-14 | PXD034529 | Pride
Chlorpyrifos oxon Protein prospector Map-rich tubulin Isopeptide Crosslinks 
ProteomicsDichlorvos-induced formation of isopeptide crosslinks between proteins in SH-SY5Y cells
Chlorpyrifos oxon catalyzes the crosslinking of proteins via an isopeptide bond between lysine and glutamic acid or aspartic acid in studies with purified proteins. Our goal was to determine the crosslinking activity of the organophosphorus pesticide, dichlorvos. We developed a protocol for examini...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD033593 | Pride
Shsy5y human neuroblastoma cells Dichlorvos Mass spectrometry Manual evaluation Protein prospector Crosslink Isopeptide Crosslinks 
ProteomicsChlorpyrifos oxon crosslinking of amyloid beta 42 peptides is a new route for generation of self-aggregating amyloidogenic oligomers that promote Alzheimer’s disease
Epidemiological evidence suggests that people chronically exposed to organophosphorus pesticides are at increased risk of neurodegenerative disease. Covalently linked amyloid beta dimers have been isolated from the brains of Alzheimer’s patients. The toxic forms of amyloid beta are amyloid dimers ...
ORGANISM(S): Homo sapiens (Human) 
2022-07-17 | PXD034163 | Pride
Chlorpyrifos oxon Amyloid beta Isopeptide Crosslinks 
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