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ProteomicsParallel Reaction Monitoring on a Q Exactive Mass Spectrometer Increases Reproducibility of Phosphopeptide Detection in Bacterial Phosphoproteomics Measurements
Increasing number of studies report the relevance of protein Ser/Thr/Tyr phosphorylation in bacterial physiology, yet the analysis of this type of modification in bacteria still presents a considerable challenge. Unlike in eukaryotes, where tens of thousands of phosphorylation events likely occupy m...
ORGANISM(S): Escherichia coli 
2018-06-05 | PXD008211 | Pride
Lc-msms Phosphoproteomics Tio2 Prm E.coli Qexactive Dda 
ProteomicsPhosphoproteome Dynamics Mediate Revival of Bacterial Spores
Background: Bacterial spores can remain dormant for decades, yet harbor the exceptional capacity to rapidly resume metabolic activity and recommence life. Although germinants and their corresponding receptors have been known for more than 30 years, the molecular events underlying this remarkable cel...
ORGANISM(S): Bacillus subtilis 
2015-08-27 | PXD002559 | Pride
Bacillus subtilis  spore  phosphoproteome  germination 
ProteomicsArginine phosphorylation during spore germination in bacteria
Arginine phosphorylation, an emerging protein modification, has been lately described to occur in B. subtilis and Staphylococcus aureus. The kinase responsible for arginine phosphorylation in B. subtilis was shown to be McsB, which counteracts the protein arginine phosphatase YwlE. Here we demonstra...
ORGANISM(S): Bacillus subtilis 
2022-03-02 | PXD011082 | Pride
Arginine phosphorylation Spore germination 
MetabolomicsHorizontal Transfer of Bacteriocin Biosynthesis Genes Requires Metabolic Adaptation To Improve Compound Production and Cellular Fitness

Biosynthetic gene clusters (BGCs) encoding the production of bacteriocins are widespread among bacterial isolates and are important genetic determinants of competitive fitness within a given habitat. Staphylococci produce a tremendous diversity of compounds, and the corresponding BGCs are frequen...

2022-11-09 | MTBLS5196 | MetaboLights
ProteomicsDeep proteogenomics of Bacillus subtilis provides insights into function and evolution of bacterial organism
"Bacillus subtilis is an aerobic, endospore forming, rod-shaped Gram-positive bacterium. It has a relatively small genome of 4,215,606 bp with 4,197 protein coding genes. It is considered a model organism to study natural phenomena, such as chromosome replication, sporulation or swarming motility. F...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2018-11-28 | PXD008860 | Pride
Phylostratigraphy" "bacillus subtilis Proteogenomics Acetylation Shotgun proteomics Phosphorylation 
ProteomicsChlorosis as developmental program in cyanobacteria: The proteomic fundament for surviving and awakening
Cyanobacteria that do not fix nitrogen adapt to prolonged periods of nitrogen deprivation in a chlorotic state where cell growth and metabolism is arrested. Upon nutrient availability, the cells return back to vegetative growth within 2-3 days. This resuscitation process is highly orchestrated and r...
ORGANISM(S): Synechocystis sp. PCC 6803 
2018-06-28 | PXD008027 | Pride
Synechocystis sp. pcc 6803 phosphoproteome dynamics during resuscitation form nitrogen-chlorosis 
ProteomicsPhosphoproteome of the cyanobacterium Synechocystis sp. PCC 6803 and its dynamics during nitrogen starvation
Cyanobacteria have shaped the earth’s biosphere as the first oxygenic photoautotrophs and still play an important role in many ecosystems. The ability to adapt to changing environmental conditions is an essential characteristic in order to ensure survival. To this end, numerous studies have shown th...
ORGANISM(S): Synechocystis sp. (strain PCC 6803 / Kazusa) 
2016-05-09 | PXD001831 | Pride
Nitrogen starvation Ltq-orbitrap Cyanobacteria Synechocystis sp. pcc 6803 Peptide dimethylation labeling Phosphoproteome 
ProteomicsA nanobody-based toolset to monitor and modify the mitochondrial GTPase Miro1
The mitochondrial outer membrane (MOM)-anchored GTPase Miro1, is a central player in mitochondrial transport and homeostasis. The dysregulation of Miro1 in amyotrophic lateral sclerosis (ALS) and Parkinson's disease (PD) suggests that Miro1 may be a potential biomarker or drug target in neuronal dis...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD031218 | Pride
Nanobody Miro1 Interactome 
ProteomicsQuantitative proteomics links the intermediate filament nestin to vemurafenib resistance in melanoma cell lines
Targeted inhibition of mutated kinases using selective MAP kinase inhibitors in malignant melanoma often results in temporary improvement of the metastatic disease followed by rapid development of resistance. To gain insights in molecular processes that govern resistance, we performed SILAC-based qu...
ORGANISM(S): Homo sapiens (Human) 
2019-05-14 | PXD010683 | Pride
Cytoskeletal proteins Mass spectrometry Drug resistance Melanoma 
ProteomicsIncorporation of nonproteinogenic amino acids into bacterial proteome analyzed by unbiased quantitative MS of protein modifications
To study incorporation of nonproteinogenic amino acids in bacterial proteome we performed a global, unbiased protein modification analysis of the E. coli K12 strain with defective editing mechanism of the leucyl tRNA synthetase (LeuRS), which in addition to leucine incorporates a nonproteinogenic am...
ORGANISM(S): Escherichia coli 
2016-07-11 | PXD003468 | Pride
Mistranslation Trna synthetase Norvaline Leucine Nonproteinogenic amino acids Orbitrap 
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