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ProteomicsSpecialisation of meiotic kinetochores revealed through a synthetic spindle assembly checkpoint strategy.
Meiosis creates haploid gametes through two sequential M phases. While many studies have focused on meiosis I, the molecular events which drive and define meiosis II are largely unknown. Here, we report a novel cell synchronization strategy which allows for collection of yeast cells arrested at meta...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-03-04 | PXD067911 | Pride
ProteomicsA functional genomics screen identifies genes required for Schizosaccharomyces pombe meiosis
Meiosis is a specialized cell division that generates gametes, such as eggs and sperm. Errors in meiosis result in miscarriages and are the leading cause of birth defects, however the molecular origins of these defects remain unknown. Studies in model organisms are beginning to identify the genes an...
ORGANISM(S): Schizosaccharomyces pombe 
2018-01-04 | PXD008245 | Pride
Schizosaccharomyces pombe Chromosome segregation Sporulation Meiosis 
ProteomicsA Siz1/Siz2-mediated SUMOylation programme inactivates the shugoshin-associated phosphatase-kinase network to stabilize sister kinetochore biorientation
The accurate segregation of chromosomes during mitosis relies on the attachment of sister chromatids to microtubules from opposite poles, called biorientation. Sister chromatid cohesion resists microtubule forces, generating tension which provides the signal that biorientation has occurred. How tens...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-03-25 | PXD019287 | Pride
Chromosome segregation Mitosis Shugoshin 
ProteomicsRewiring of the phosphoproteome executes two meiotic divisions
The cell cycle is ordered by a controlled network of kinases and phosphatases. To generate gametes via meiosis, two distinct and sequential chromosome segregation events occur without an intervening S phase. How canonical cell cycle controls are modified for meiosis is not well understood. Here, usi...
ORGANISM(S): Saccharomyces cerevisiae Saccharomyces cerevisiae (Baker's yeast) 
2026-09-12 | PXD045368 | Pride
Spo13 Saccharomyces cerevisiae Kinases Phosphorylation Meiosis Cell cycle 
ProteomicsReductional meiosis I chromosome segregation is established by coordination of key meiotic kinases
Meiosis produces gametes through a specialised, two-step cell division, which is highly error-prone in humans. Reductional meiosis I, where maternal and paternal chromosomes (homologs) segregate, is followed by equational meiosis II, where sister chromatids separate. Uniquely during meiosis I, siste...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-11-13 | PXD012627 | Pride
Chromosome segregation Lc-ms Kinases Meiosis 
ProteomicsThe proteomic landscape of centromeric chromatin reveals an essential role for the Ctf19 complex in meiotic kinetochore assembly.
Faithful gamete formation during meiosis requires that kinetochores take on new functions that impact homolog pairing/recombination and their attachments to microtubules. We used a proteomics pipeline to determine the global composition of centromeric chromatin and kinetochores at distinct meiotic s...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-02-23 | PXD019754 | Pride
Kinetochores Saccharomyces cerevisiae Meiosis Chromatin Label-free quantitation. 
ProteomicsRewiring of the phosphoproteome executes two meiotic divisions
The cell cycle is ordered by a controlled network of kinases and phosphatases. To generate gametes via meiosis, two distinct and sequential chromosome segregation events occur without an intervening S phase. How canonical cell cycle controls are modified for meiosis is not well understood. Here, usi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-03-21 | PXD048618 | Pride
Spo13 Saccharomyces cerevisiae Kinases Phosphorylation Meiosis 
ProteomicsNon-canonical functions of spindle checkpoint proteins in meiosis
Gametes are produced via meiosis, a specialized cell division associated with frequent errors which cause birth defects and infertility. Uniquely in meiosis I, homologous chromosomes segregate to opposite poles, usually requiring their linkage by chiasmata, the products of crossover recombination1....
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-07-18 | PXD048251 | Pride
Chromosome segregation Mad3 Mad2 Saccharomyces cerevisiae Meiosis Spindle checkpoint 
ProteomicsMolecular mechanism targeting condensin for chromosome condensation
Faithful chromosome segregation requires packaging of the genome on both global and local scales. Condensin plays a crucial role at pericentromeres to resist spindle forces and ensure the oriented attachment of kinetochores to microtubules at mitosis. Here we demonstrate that budding yeast condensin...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-12-10 | PXD056626 | Pride
Chromosome segregation Dia Shugoshin Ipms Pericentromeres Condensin 
ProteomicsMolecular mechanism targeting condensin for chromosome condensation.
Faithful chromosome segregation requires packaging of the genome on both global and local scales. Condensin plays a crucial role at pericentromeres to resist spindle forces and ensure the oriented attachment of kinetochores to microtubules at mitosis. Here we demonstrate that budding yeast condensin...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-12-10 | PXD056649 | Pride
Condensin Chromosome segregation Slim Cross-linking mass spectrometry Shugoshin 
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