This study aimed to enhance the therapeutic effect of MSC-derived extracellular vesicles (EVs) by overexpressing the TSG-6 gene using CRISPR activation and evaluate the biological activity of these modified EVs on human intervertebral disc (IVD) cells in vitro. n immortalized human MSC line was tran...
Repressor-activator protein 1 (scRap1) is the major binding activity at Saccharomyces cerevisiae telomeres, with roles in telomere length regulation and establishment of subtelomeric silencing by recruiting the Sir proteins. scRap1 also acts as a transcription factor controlling the expression of ri...
Validation of a FAM98C KO in HEK Flp-In T-REx 293 cells by quantifying the occurrence of FAM98C-specific peptides in whole cell lysates with LC-MSMS. WT HEK Flp-In T-REx 293 cells were used as control.
TP53 mutations across multiple cancers, including acute myeloid leukemia (AML), are associated with poor outcomes irrespective of treatment modality. However, druggable vulnerabilities beyond canonical p53 targets remain largely unexplored. We identify BIRC5(encodes survivin), an inhibitor of the ap...
Here we show that the histone methyltransferase MLL4 (Kmt2d) is required for stem cell activity and an aggressive form of acute myeloid leukemia (AML) harboring the MLL-AF9 oncogene. MLL4 exerts its function by regulating transcriptional programs associated with the anti-oxidant response. The role o...
Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes in a fast and reproducible manner. Here, we present an ultra-fast scanning data-independent acquisition (DIA) strategy consisting on 2-Th precursor isolation windows, dissolving the differences between data-dependen...
