Sort   by:  
 Page size 
ProteomicsThe $10 proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers
Mass spectrometry (MS)-based proteomics remains technically demanding and prohibitively expensive for many large-scale or routine applications, with per-sample costs of hundreds of dollars or more. To democratize access to proteomics and facilitate its integration into more high-throughput multiomic...
ORGANISM(S): Homo sapiens (Human) 
2026-02-04 | PXD066701 | Pride
Limited sample input $10 proteome 
ProteomicsProteome-wide structural changes measured with limited proteolysis-mass spectrometry: an advanced protocol for high-throughput applications.
Proteins regulate biological processes by changing their structure or abundance to accomplish a specific function. In response to any perturbation or stimulus, protein structure may be altered by a variety of molecular events, such as post translational modification, protein-protein interaction, agg...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-01-10 | PXD031627 | Pride
Alpha-synuclein Shotgun proteomics Yeast Structural proteomics Lip-ms 
ProteomicsCapillary electrophoresis coupled to electrospray ionization tandem mass spectrometry (CE-MS/MS) for ultra-sensitive proteomic analysis of limited sample
In this work, we developed an ultra-sensitive CE-MS/MS method for bottom-up proteomics analysis of limited samples, down to sub-nanogram levels of total protein. Analysis of 880 pg and 88 pg of HeLa protein digest standard by CE-MS/MS yielded ~1,100±46 and ~160±59 proteins, respectively, demonstrati...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027226 | Pride
Ultra-low flow liquid phase separation Ce-ms Ultra-sensitive proteomics Monolithic columns Capillary electrophoresis Rp-nlc-ms 
UnknownTriboelectric Nanogenerator-Coated Blade Spray Mass Spectrometry for Volume-Limited Drug Analysis.
Not available
S-EPMC10653212 | biostudies-literature
ProteomicsProteome-wide structural changes measured with 2 limited proteolysis-mass spectrometry: an advanced 3 protocol for high-throughput applications.
Proteins regulate biological processes by changing their structure or abundance to accomplish a specific function. In response to any perturbation or stimulus, protein structure may be altered by a variety of molecular events, such as post translational modification, protein-protein interaction, agg...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-01-10 | PXD031616 | Pride
Shotgun proteomics Human alpha-synuclein Yeast Structural proteomics Lip-ms 
MetabolomicsMetabolic phenotyping of urine from a dementia research cohort
To better understand the molecular mechanisms underpinning physiological variation in human populations, metabolic phenotyping approaches are increasingly being applied to studies involving hundreds and thousands of biofluid samples. Hyphenated ultra-performance liquid chromatography-mass spectromet...
2022-03-01 | MTBLS719 | MetaboLights
UnknownSub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples.
Not available
S-EPMC7648103 | biostudies-literature
ProteomicsLowering Sample Requirements to Study Tyrosine Kinase Signaling using Phosphoproteomics with the TMT Calibrator Approach
Analysis of tyrosine kinase signaling is critical for the development of targeted cancer therapy. Currently, immunoprecipitation (IP) of phosphotyrosine (pY) peptides prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used to profile kinase substrates. A typical protocol request...
ORGANISM(S): Homo sapiens (Human) 
2020-07-29 | PXD019764 | Pride
Signaling Phosphotyrosine Label free quantification Lung cancer Lc-ms/ms 
ProteomicsLowering Sample Requirements to Study Tyrosine Kinase Signaling using Phosphoproteomics with the TMT Calibrator Approach
Analysis of tyrosine kinase signaling is critical for the development of targeted cancer therapy. Currently, immunoprecipitation (IP) of phosphotyrosine (pY) peptides prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used to profile kinase substrates. A typical protocol requests ...
ORGANISM(S): Homo sapiens (Human) 
2020-07-29 | PXD018952 | Pride
Signaling Tmt Cancer Calibrator Tyrosine phosphorylation 
ProteomicsNanoTPOT: Enhanced Sample Preparation for Quantitative Na-noproteomic Analysis (revision work)
With the ever-growing need for protein-level understanding in pathological research, proteomics researchers thrive to exam-ine detailed proteome dynamics using crucial, yet often limited, primary and clinical samples. Aside from mass spectrometer instrumentation advancement, a single-tube-based high...
ORGANISM(S): Homo sapiens (Human) 
2021-03-16 | PXD018247 | Pride
Hela Tmt 
Sort   by:  
 Page size