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TranscriptomicsSCL and LMO1 reprogram thymocytes into self-renewing cells.
The SCL and LMO1 oncogenic transcription factors reprogram thymocytes into self-renewing pre-leukemic stem cells (pre-LSCs). Here we report that SCL directly interacts with LMO1 to activate the transcription of a self-renewal program coordinated by LYL1. Gene expression profiles of thymocytes from S...
ORGANISM(S): Mus musculus 
2015-11-05 | E-GEOD-74659 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse double negative thymocytes
In order to understand the molecular mechanisms of DN thymocyte development, it may be also of use to clarify how these developmental processes are regulated in terms of their entire gene expression, to which cell differentiation is ultimately ascribed. In the current study, we approached this iss...
ORGANISM(S): Mus musculus 
2008-06-15 | E-GEOD-7784 | biostudies-arrayexpress
ProteomicsMass spectrometry analysis of the Themis1 interactome in murine thymocytes
Themis1, a recently described T-lineage specific protein, is essential for thymic positive and negative selection. Although Themis1 has been clearly identified as a component of the T cell antigen receptor (TCR) signalosome, its precise role in TCR signaling remains unclear. Here, we used quantitati...
ORGANISM(S): Mus musculus (Mouse) 
2017-02-06 | PXD004072 | Pride
Thymocytes Ap-ms Tcr signalling Themis1 Interactome 
TranscriptomicsTranscription profiling of mouse double-positive (CD4+CD8+) thymocytes following dexamethasone treatmen
Glucocorticoids play a role in regulation of T lymphocytes homeostasis and development. In particular, glucocorticoid treatment induces massive apoptosis of CD4+CD8+ double positive (DP) thymocytes. This effect is due to many mechanisms, mainly driven by modulation of gene transcription. To find out...
ORGANISM(S): Mus musculus 
2008-06-13 | E-GEOD-5463 | biostudies-arrayexpress
ProteomicsAnalysis of CD5 signalosome in primary T cells
CD5 is characterized as an inhibitory co-receptor with important regulatory role during T cell development. To study the molecular mechanism by which CD5 operates, we used quantitative mass spectrometry to analyze the components of the CD5 signaling machinery in primary T cells. In a first set of ex...
ORGANISM(S): Mus musculus (Mouse) 
2020-05-18 | PXD017343 | Pride
Tyrosine phosphorylation Cd5 Interactomics Tcr 
TranscriptomicsReal-time quantitative PCR analysis of mouse mature CD4SP thymocytes
RNA was isolated with the RNeasy kit (Qiagen) from electronically sorted mature CD4SP thymocytes. RNA was reverse transcribed into cDNA by oligo(dT) priming with the QuantiTect Reverse transcription kit (Qiagen). The expression of 84 key genes involved in apoptosis, or programmed cell death was perf...
ORGANISM(S): Mus musculus 
2013-07-27 | E-GEOD-49271 | biostudies-arrayexpress
Genomicsmouse thymocytes
mouse thymocytes
PRJNA1013063 | ENA
TranscriptomicsReal-time quantitative PCR analysis of mouse immature CD4SP thymocytes
RNA was isolated with the RNeasy kit (Qiagen) from electronically sorted immature CD4SP thymocytes. RNA was reverse transcribed into cDNA by oligo(dT) priming with the QuantiTect Reverse transcription kit (Qiagen). The expression of 84 key genes involved in apoptosis, or programmed cell death was pe...
ORGANISM(S): Mus musculus 
2013-07-27 | E-GEOD-49270 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of mouse total CD4SP thymocytes
RNA was isolated with the RNeasy kit (Qiagen) from electronically sorted total CD4SP thymocytes. RNA was reverse transcribed into cDNA by oligo(dT) priming with the QuantiTect Reverse transcription kit (Qiagen). The expression of 84 key genes involved in apoptosis, or programmed cell death was perfo...
ORGANISM(S): Mus musculus 
2013-07-27 | E-GEOD-49264 | biostudies-arrayexpress
TranscriptomicsEffect of c-Myb deficiency on pre-selection DP thymocytes
Comparing the mRNA expression profiles of c-Myb deficient and c-Myb sufficient Tcra-/- DP thymocytes. Results provide insight into the role of c-Myb in the regulation of survival and differentiation during the pre-selection DP stage where c-Myb expression is abundant during T cell development. DP th...
ORGANISM(S): Mus musculus 
2010-01-21 | E-GEOD-19528 | biostudies-arrayexpress
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