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Thirty commercial proteins were prepared at 1.5 pmol/µL in three different subsets of 10 proteins each. Proteins from these subsets were spiked to a 15-µg Escherichia coli background in different amounts and five different mixtures were prepared in triplicates. The final amount of each protein in ea...
ORGANISM(S): Escherichia coli 
2019-07-19 | PXD005642 | Pride
In this project, Q-Exactive HF(Thermo Fisher Scientific,San Jose,CA) was used to acquire mass spectrometry (MS) data for 7 samples in Data Independent Acquisition (DIA) mode. Quantification of peptides and proteins was performed using Spectronaut™ and MSstats software packages
ORGANISM(S): Rattus norvegicus (Rat) 
2025-12-01 | PXD032395 | Pride
Mass spectrometry-based proteomics with data-independent acquisition benefits from advanced instrumentation and computational analysis. Despite continued im- provements, the quality of quantification may be poor for some measurements. As the scale of proteomic experiments increases, these poor-quali...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-07-23 | MSV000098622 | MassIVE
This study involves the identification of specific Stag1 interactors with and without Cohesin loss. Stag1 was immunoprecipiated from human HCT116 cells, under 4 treatments - untreated (UTR), Non-specific siRNA treatment (SiCON), SA1 siRNA treatment (SiSA1) and Auxin treatment for Cohesin loss (IAA)....
ORGANISM(S): Homo sapiens (Human) 
2023-04-18 | PXD024354 | Pride
This study involves the identification of specific interactors for STAG2ex32+ and STAG2ex32delta. Plasmids expressing YFP-STAG2 isoforms were transfected into HCT116OsTIR cells using untransfected cells as mock control. 40h post transfection, whole cell lysates were extracted and STAG2 isoforms were...
ORGANISM(S): Homo sapiens (Human) 
2023-05-04 | PXD041975 | Pride
Vasculogenic therapies have been investigated for the treatment of peripheral artery disease (PAD) with very limited success in clinical trials. The isoform PP2A (protein phosphatase 2)/B55alpha inhibits the activity of the prolyl hydroxylase 2 (PHD2) and activates the hypoxia inducing factor-1alpha...
ORGANISM(S): Mus musculus (Mouse) 
2024-04-03 | PXD050360 | Pride
The GASH/Sal hamster (Genetic audiogenic seizure, Salamanca) is a model of audiogenic seizures with the epileptogenic focus localized in the inferior colliculus (IC). The sound-induced seizures exhibit a short latency (7-9 seconds), which implies innate protein disturbances in the IC as a basis for ...
ORGANISM(S): Mesocricetus auratus (Golden hamster) 
2023-05-10 | PXD039755 | Pride
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