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ProteomicsMucin-binding proteins of E. coli membrane
Proteins from E. coli outer membrane were extracted with carbonate method, separated in 2D-PAGE and transferred onto nitrocellulose membrane. After incubation with flourescent-labelled mucin, mucin-binding protein spots were cut out, subjected to trypsinolysis and identified by protein analysis wit...
ORGANISM(S): Escherichia coli 
2019-12-16 | PXD010472 | Pride
Escherichia coli Hplc-ms-ms Mucin binding Membrane 
TranscriptomicsCross-feeding by Bifidobacterium breve UCC2003 during Bifidobacterium bifidum PRL2010 growth on a mucin-based medium.
Bifidobacteria constitute commensal bacteria that commonly inhabit the mammalian gastro intestinal tract. The gut commensal Bifidobacterium breve UCC2003 was previously shown to utilise a variety of plant/diet-derived carbohydrates, including cellodextrin, starch and galactan. In the current study, ...
ORGANISM(S): Bifidobacterium breve UCC2003 
2014-12-17 | E-GEOD-59013 | biostudies-arrayexpress
TranscriptomicsA GNAS mutation found in pancreatic intraductal papillary mucinous neoplasms induces drastic alterations of gene expression profiles with upregulation of mucin genes.
GNAS, a gene encoding G-protein stimulating alpha subunit, is frequently mutated in intraductal papillary mucinous neoplasms (IPMNs), which is an indolent and slow-growing pancreatic neoplasm that secretes abundant mucin. GNAS mutation is not observed in conventional ductal adenocarcinomas of the pa...
ORGANISM(S): Homo sapiens 
2014-02-04 | E-GEOD-53350 | biostudies-arrayexpress
ProteomicsHuman plasma proteins detected by HPLC-MS/MS after blood incubation with E. coli treated/not treated with mucin
Whole human blood incubated with E. coli isolate with or without bound mucin. Plasma was obtained and the effect of mucin bound to E. coli on plasma proteins content was analysed by HPLC-MS-MS.
ORGANISM(S): Homo sapiens (Human) 
2019-12-16 | PXD010496 | Pride
Plasma Mucin E. coli 
TranscriptomicsTranscription profiling of mouse intestine in ENU mutagenesis mouse strains with missense mutations in Muc2 mucin and ER stress
Background; MUC2 mucin produced by intestinal goblet cells is the major component of the intestinal mucus barrier. MUC2 homo-oligomerizes intracellularly into large secreted polymers which give mucus its viscous properties. The inflammatory bowel disease (IBD) ulcerative colitis is characterized by...
ORGANISM(S): Mus musculus 
2008-04-06 | E-GEOD-9913 | biostudies-arrayexpress
ProteomicsBinding of Akkermansia muciniphila to mucin is O-glycan specific
The intestinal anaerobic bacterium Akkermansia muciniphila is specialized in the degradation of mucins, which are heavily O-glycosylated proteins that constitute the major components of the mucus lining the intestine. Despite that adhesion to mucins is considered critical for the persistence of A. m...
ORGANISM(S): Homo sapiens (Human) 
2024-05-30 | PXD051738 | Pride
Akkermansia muciniphila Mucin O-glycan 
ProteomicsStructure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences
Akkermansia muciniphila, a mucin-degrading microbe found in the human gut, is often associated with positive health outcomes. The abundance of Akkermansia muciniphila is modulated by the presence and accessibility of nutrients, which can be derived from diet or host glycoproteins. In particular, the...
ORGANISM(S): Homo sapiens (Human) 
2022-08-28 | PXD031225 | Pride
X-ray crystallography Mucin Mucinase Microbiome O-glycosylation Molecular docking Enzyme structure Metalloprotease 
TranscriptomicsSpecific modulation of mucosal immune response, tolerance and proliferation in mice colonized with A. muciniphila
Epithelial cells of the mammalian intestine are covered with a mucus layer that prevents direct contact with intestinal microbes but also constitutes a substrate for mucus-degrading bacteria. To study the effect of mucus degradation on the host response, germ-free mice were colonized with Akkermansi...
ORGANISM(S): Mus musculus 
2011-09-20 | E-GEOD-18587 | biostudies-arrayexpress
TranscriptomicsFungal allergen extracts-induced gene expression in airway epithelial cells (NCI-H292)
We compared the gene expression stimulated with fungal extracts from Aspergillus (A.) fumigatus, Alternaria (A.) alternata, or Penicillium (P.) notatum in NCI-H292 (a human bronchial epithelial cell line) to search Allergic bronchopulmonary mycosis (ABPM)-related genes. We identified a mucin-related...
ORGANISM(S): Homo sapiens 
2011-07-07 | E-GEOD-22323 | biostudies-arrayexpress
ProteomicsDesign of a mucin-selective protease for targeted degradation of cancer-associated mucins
Targeted protein degradation is an emerging strategy for the elimination of classically undruggable proteins. Here, to expand the landscape of targetable substrates, we designed degraders that achieve substrate selectivity via recognition of a discrete peptide- and glycan- motif and achieve cell-typ...
ORGANISM(S): Homo sapiens (Human) 
2023-07-21 | PXD042243 | Pride
Mucins Glycoprotease Targeted degradation Tails 
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