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ProteomicsSpatial distribution of endogenous tissue protease activity in gastric carcinoma mapped by MALDI mass spectrometry imaging
Aberrant protease activity has been implicated in the etiology of various prevalent diseases including neurodegeneration and cancer, in particular metastasis. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has recently been established as a key technology for bio...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-25 | PXD010369 | Pride
Gastric mouse carcinoma Lc-ms Sp3 Proteases Maldi 
ProteomicsEZHIP / CXorf67 mimics K27M mutated oncohistones and functions as an intrinsic inhibitor of PRC2 function in aggressive posterior fossa ependymoma
Posterior fossa A (PFA) ependymomas comprise one out of nine molecular groups of ependymoma. PFA tumors are mainly diagnosed in infants and young children, show a poor prognosis and are characterized by a lack of the repressive histone H3 lysine 27 trimethylation (H3K27me3) mark. Recently, we report...
ORGANISM(S): Homo sapiens (Human) 
2019-07-08 | PXD012318 | Pride
Prc2 Ezhip Cxorf67 Ependymoma 
ProteomicsFunctional states in tumor-initiating cell differentiation in human colorectal cancer
Intra-tumor heterogeneity of tumor-initiating cell (TIC) activity drives colorectal cancer (CRC) progression and therapy resistance. Here, we used single-cell mRNA-sequencing (scRNA-seq) of patient-derived CRC models to decipher distinct cell subpopulations based on their transcriptional profiles. C...
ORGANISM(S): Homo sapiens (Human) 
2021-05-07 | PXD018230 | Pride
Tumor heterogeneity Transcriptional programs Colorectal cancer Tumor cell differentiation Patient-derived cancer models Tumor-initiating cells Tumor metabolism Single-cell rna-sequencing 
ProteomicsAutomated sample preparation with SP3 for low-input clinical proteomics
High-throughput and streamlined workflows are essential in clinical proteomics for standardized processing of samples originating from a variety of sources, including frozen tissue, FFPE tissue, or blood. To reach this goal, we have implemented single-pot solid-phase-enhanced sample preparation (SP3...
ORGANISM(S): Homo sapiens (Human) 
2020-01-10 | PXD014556 | Pride
Sp3 Clinical proteomics Pulmonary adenocarcinoma Sample preparation Low-input High-throughput Automation 
ProteomicsSystematic Evaluation of Protein Reduction and Alkylation reveals Massive Unspecific Side Effects by Iodine-containing Reagents
In this study, we systematically evaluated reduction and alkylation of proteins using three reducing agents (DTT, TCEP, and BME) in combination with four alkylation agents (IAA, IAC, AA, and CAA). We tested these conditions using strong anion exchange (SAX) fractionated in-solution digests and in-ge...
ORGANISM(S): Homo sapiens (Human) 
2017-05-30 | PXD005183 | Pride
Alkylation Proteomics Reduction 
ProteomicsAutomated sample preparation with SP3 for low-input clinical proteomics
High-throughput and streamlined workflows are essential in clinical proteomics for standardized processing of samples originating from a variety of sources, including frozen tissue, FFPE tissue, or blood. To reach this goal, we have implemented single-pot solid-phase-enhanced sample preparation (SP3...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2020-03-27 | PXD015840 | Pride
Fresh-frozen tissue Proteomics Sp3 Cells Sample preparation 
ProteomicsEnhancing the cardiac pig transcriptome by long-read sequencing and in-depth proteomics
Clinically translatable large animal models have become indispensable for cardiovascular research, clinically relevant proof of concept studies and for novel diagnostic and therapeutic interventions. In particular, the pig as emerged as an essential cardiovascular disease model, because its heart, c...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2020-10-29 | PXD018985 | Pride
Pig Tmt Annotation Proteomics Transcriptomics Cardiac Nanopore Lcms 
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