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ProteomicsSILAC-based quantitative proteomics of endoplasmic reticulum stress
ER stress and the unfolded protein response (UPR) involve extensive proteome remodeling in many cell compartments. However, a comprehensive analysis has been lagging due to technological limitations. Here we employ SILAC-based proteomics to quantify over 6,200 proteins at increasing concentrations o...
ORGANISM(S): Homo sapiens (Human) 
2019-11-01 | PXD013496 | Pride
Unfolded protein response Proteomics Silac Tunicamycin Endoplasmic reticulum stress 
ProteomicsPlasma proteome profiling of healthy subjects undergoing bed rest reveals unload-dependent changes linked to muscle atrophy.Plasma proteome profiling of healthy subjects undergoing bed rest reveals unload-dependent changes linked to muscle atrophy.
Inactivity and unloading. induce skeletal muscle atrophy, loss of strength and detrimental metabolic effects. We used mass spectrometry-based proteomics to measure the abundance changes of proteins circulating in the blood plasma of young healthy subject undergoing ten days of continuous bed rest. S...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD032969 | Pride
Bed rest Proteomics Plasma Skeletal muscle Cachexia Atrophy 
ProteomicsSingle muscle fiber proteomics reveals fiber type-specific features of human muscle aging
Skeletal muscle is a key tissue in human aging, which affects different muscle fiber types unequally. We developed a highly sensitive single muscle fiber proteomics workflow to study human aging and show that the senescence of slow and fast muscle fibers is characterized by diverging metabolic and p...
ORGANISM(S): Homo sapiens (Human) 
2017-06-14 | PXD006182 | Pride
Muscle fibers Skeletal muscle Aging 
ProteomicsYeast homologs of MCUR1 regulate mitochondrial proline metabolism
MCUR1 is an evolutionarily conserved mitochondrial protein that has been shown to act as a scaffold factor for the assembly of the mammalian mitochondrial calcium uniporter (MCU). Surprisingly, many fungi that do not have MCU contain MCUR1 homologs, suggesting these proteins possess alternate functi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-09-30 | PXD015476 | Pride
Proline metabolism Mcur1 Mitochondrion 
ProteomicsSingle muscle fiber proteomics reveals remodeling of focal adhesions in response to muscle unloading and microgravity
Spaceflight causes loss of muscle mass and strength, metabolic remodeling and insulin resistance, contributing to severe challenges for astronaut health. We used highly sensitive proteomics to detail single fiber type-specific molecular remodeling caused by muscle unloading in subjects undergoing pr...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD028435 | Pride
Proteomics Microgravity Muscle 
ProteomicsCalsequestrins make the difference in adult zebrafish brain
Aim of the study was to examine the expression of calsequestrin isoforms in zebrafish adult brain. By quantitative PCR we found three RNA transcripts coding for Casq1a, Casq1b and Casq2. In brain homogenate, two protein isoforms were detected by western blotting and mass spectrometry. Whole brain fr...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2020-05-27 | PXD015577 | Pride
Calsequestrin Danio rerio Cerebellum Adult 
ProteomicsMuscle proteomics of mitochondrial disorders
Mitochondrial DNA mutations progressively compromise the respiratory chain of skeletal muscle, resulting in a mosaic of metabolically healthy and defective fibers. This heterogeneity is the best diagnostic signpost of mitochondrial disorders. However, the single fiber investigation of its molecular...
ORGANISM(S): Homo sapiens (Human) 
2019-11-15 | PXD010489 | Pride
Laser microdissection Single muscle fibers Q exactive hf Human muscle Mitochondrial disorders 
ProteomicsProteomic analysis reveals blunted effect of sprint interval training by vitamin C and E treatment in both type 1 and type 2 muscle fibres
We hypothesized that SIT causes oxidative stress preferentially in fast-twitch type 2-fibres and that this stress constitutes an important trigger of mitochondrial adaptations towards increased oxidative capacity and hence improved muscular endurance. In addition, we hypothesized that the SIT-induce...
ORGANISM(S): Homo sapiens (Human) 
2025-07-29 | PXD055360 | Pride
Proteomics Sprint interval training Muscle fibers 
ProteomicsProteomics analysis of tibialis anterior muscle and descending thoracic aorta from Mustn1 whole-body knockout mice and wild-type littermates
Skeletal muscle is a highly adaptive tissue that changes with many physiological stimuli and is composed of many cell types. Upon muscle injury, the concerted action of these cells is required to proceed through the process of inflammation, extracellular matrix remodeling, and restoration of functio...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-24 | PXD046610 | Pride
Proteomics Aorta Skeletal muscle Mustn1 
ProteomicsHAX1 controls mitochondrial proteostasis
The relevance of mitochondrial proteostasis for the differentiation and function of hematopoietic and immune cells is unknown. Dissecting the HCLS1-associated protein X-1 (HAX1) –related protein network, we define a functional CLPB-HAX1-PRKD2-HSP27 axis with critical importance for the differentiati...
ORGANISM(S): Homo sapiens (Human) 
2022-05-19 | PXD023790 | Pride
Inborn errors of immunity Severe congenital neutropenia Protein quality control Hematopoiesis 
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