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ProteomicsTBK1 phosphorylates NAP1 on several sites
NAP1 is known to be phosphorylated on several sites by TBK1. We decided to identify the phosphorylation sites on NAP1. For this, using an anti-GFP antibody, we immunoprecipitated a version of NAP1 fused to the GFP protein at its amino terminus (GFP-NAP1) from HEK293T cells co-expressing GFP-NAP1 and...
ORGANISM(S): Homo sapiens (Human) 
2026-07-16 | PXD062298 | Pride
Tbk1 Nap1 Azi2 Phosphorylation 
ProteomicsNAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis.
Supporting raw MS data for paper (DOI:10.1083/jcb.202303082) by Paul S. et al., titled "NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis". Index of MS supporting files uploaded: - Related to Figure 6A;S4A (AO3556-AO3579: whole proteome (Unimod: 35; 2016; 4)). -...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-01 | MSV000093220 | MassIVE
ProteomicsIdentification of phosphorylation sites on NAP1/AZI2
We observed smearing for the TBK1-adaptor protein NAP1/AZI2 during mitophagy. To verify whether this smearing was due to phosphorylation by TBK1, we performed an in vitro kinase assay and complemented this with the immunoprecipitation of NAP1 from HAP1, whose genetic background (FIP200 knockout) res...
ORGANISM(S): Homo sapiens (Human) 
2024-04-12 | PXD049184 | Pride
Azi2 Nap1 Mitophagy Tbk1 
TranscriptomicsHistone chaperone NAP1 is required for sister chromatid resolution
The fidelity of chromosome duplication through cell divisions requires timely binding and release of the cohesin. Cohesin is a ring-shaped protein complex linking newly replicated sister chromatids to ensure their appropriate transmission through mitosis. Upon commencement of mitosis cohesin is remo...
ORGANISM(S): Drosophila melanogaster 
2012-07-25 | E-GEOD-30938 | biostudies-arrayexpress
GenomicsErythrobacter sp. NAP1
An aerobic, bacteriochlorophyll a-producing bacteria isolated from the northwest Atlantic Ocean
PRJNA13512 | ENA
GenomicsHistone chaperone NAP1 is required for sister chromatid resolution
The fidelity of chromosome duplication through cell divisions requires timely binding and release of the cohesin. Cohesin is a ring-shaped protein complex linking newly replicated sister chromatids to ensure their appropriate transmission through mitosis. Upon commencement of mitosis cohesin is remo...
ORGANISM(S): Drosophila melanogaster 
2012-07-26 | GSE30938 | GEO
ProteomicsNAP1-RELATED PROTEINS 1 and 2 negatively regulate H2A.Z abundance in chromatin
LC-MS/MS analysis of NAP1-RELATED PROTEIN1 and 2 affinity purifications from Arabidopsis thaliana
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-02-19 | MSV000084979 | MassIVE
GenomicsA genome wide role for CHD remodeling factors and Nap1 in nucleosome disassembly
Chromatin remodeling factors and histone chaperones were previously shown to cooperatively affect nucleosome assembly and disassembly processes in vitro. Here we show that S. pombe CHD remodellers, Hrp1 and Hrp3 physically interact with the histone chaperone Nap1. Genome wide analysis of Hrp1, Hrp3 ...
ORGANISM(S): Schizosaccharomyces pombe 
2007-05-19 | GSE6557 | GEO
UnknownRedundant Functions for Nap1 and Chz1 in H2A.Z Deposition.
Not available
S-EPMC5589762 | biostudies-literature
UnknownControl of mitotic events by Nap1 and the Gin4 kinase.
Not available
S-EPMC2139941 | biostudies-literature
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