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ProteomicsImproved Single Cell Proteome Coverage Using Narrow-Bore Packed NanoLC Columns and Ultrasensitive Mass Spectrometry
we report on improved single-cell proteome coverage through the combination of the previously developed Nanodroplet Processing in One Pot for Trace Samples (nanoPOTS) platform with further miniaturization of liquid chromatography (LC) separations and implementation of an ultrasensitive latest-genera...
ORGANISM(S): Homo sapiens (Human) 
2020-02-26 | PXD016921 | Pride
Eclipse Narrow-bore lc Single cell 
ProteomicsOpen-Tubular Trap Columns: Towards Simple and Robust Liquid Chromatography Separations for Single-Cell Proteomics
Nanoflow liquid chromatography-mass spectrometry is key to enabling in-depth proteome profiling of trace samples such as single cells, but these separations can lack robustness due to the use of narrow-bore columns that are susceptible to clogging. In the case of single-cell proteomics, offline clea...
ORGANISM(S): Homo sapiens (Human) 
2024-02-09 | PXD048565 | Pride
Solid-phase extraction Trap column Open-tubular Single-cell proteomics 
ProteomicsImproved sensitivity of ultra-low flow LC-MS-based proteomic profiling of limited samples using monolithic capillary columns and FAIMS technology
In this work, we pioneered a combination of ultra-low flow (ULF) high-efficiency ultra-narrow bore monolithic LC columns coupled to MS via a high-field asymmetric waveform ion mobility spectrometry (FAIMS) interface to evaluate the potential applicability for high sensitivity, robust and reproducibl...
ORGANISM(S): Homo sapiens (Human) 
2021-01-10 | PXD020669 | Pride
Faims Lc-ms Proteomic analysis Ultra-low flow lc Monolithic column 
UnknownImproved Single-Cell Proteome Coverage Using Narrow-Bore Packed NanoLC Columns and Ultrasensitive Mass Spectrometry.
Not available
S-EPMC7550239 | biostudies-literature
ProteomicsHigh-flow LC-MS/MS analyses - Conventional-Flow Liquid Chromatography-Mass Spectrometry for Exploratory Bottom-Up Proteomic Analyses
The major objective of our study was to find out what are the extra costs and what optimization and adjustment steps of a high-flow LC-MS system have to be undertaken to identify a comparable number of proteins as on a nanoLC-MS system.
ORGANISM(S): Homo sapiens (Human) 
2019-11-07 | PXD005481 | Pride
Microbore column Hela High-flow Narrow-bore column 
UnknownIntegrated Biomimetic 2D-LC and Permeapad® Assay for Profiling the Transdermal Diffusion of Pharmaceutical Compounds.
Not available
S-EPMC12843750 | biostudies-literature
UnknownValidation and implementation of liquid chromatographic-mass spectrometric (LC-MS) methods for the quantification of tenofovir prodrugs.
Not available
S-EPMC5835418 | biostudies-literature
ProteomicsProbing molecular diversity of brain cells with chemically modified aptamers
We developed a volumetric correlated light and electron microscopy (CLEM) approach that uses slow off-rate modified aptamers (SOMAmers) for fluorescence labeling of cells in ultrastructurally reconstructable electron micrographs. The small size of aptamers enables intracellular labeling in fixed bra...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2026-04-17 | PXD075602 | Pride
Neuroscience Pulldown ms 
MetabolomicsUntargeted In Silico Compound Classification - A Novel Metabolomics Method to Assess the Chemodiversity in Bryophytes

In plant ecology, biochemical analyses of bryophytes and vascular plants are often conducted on dried herbarium specimen as species typically grow in distant and inaccessible locations. Here, we present an automated in silico compound classification framework to annotate metabolites using an unta...

2026-01-30 | MTBLS851 | MetaboLights
ProteomicsChinese giant salamander liver LC-MS/MS
Chinese giant salamander Andrias davidianus has strong tolerance to starvation. Here, a high-throughput proteomic analysis was carried out on liver samples obtained from the A. davidianus after 3, 7 and 11 months of fasting.s a result, the expression levels of 364 proteins were significantly changed...
ORGANISM(S): Andrias davidianus 
2020-01-17 | PXD014924 | Pride
Chinese giant salamander Fasting Liver Lc-ms/ms 
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