Sort   by:  
 Page size 
Metabolomics13C-glucose tracing analysis of IFN-treated A549 cells expressing wild-type SARS-CoV-2 Nsp3-4 or a deISGylation-defective Nsp3-4 mutant protein

Interferon (IFN)-stimulated gene 15 (ISG15) regulates diverse cellular processes, including antiviral immunity, through its conjugation to target proteins (ISGylation). Increasing evidence suggests that ISGylation can also reshape cellular metabolism; however, how viruses counteract ISGylation-me...

2026-05-05 | MTBLS14030 | MetaboLights
ProteomicsBiochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies
Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is the largest and plays important roles in the viral life cycle. Being a large, multidomain, transmembrane protein, Nsp3 has been the most challenging to characterize. Encoded within the multidomain Nsp3 is the papain-like proteas...
ORGANISM(S): Homo sapiens (Human) 
2021-09-15 | PXD022904 | Pride
Ubiqutylation Isg15 Sars cov-2 
ProteomicsSARS-CoV-2 Nsp3 interactome in Caco-2 cells during authentic infection
The nonstructural protein 3 (Nsp3) of SARS-CoV-2 is a large, multi-domain protein that localizes to double-membrane vesicles (DMVs), the physical site where SARS-CoV-2 (and other coronaviruses) replicate inside mammalian cells. One of the domains of Nsp3 is PLpro (papain-like protease), which harbor...
ORGANISM(S): Homo sapiens (Human) 
2026-05-05 | PXD068796 | Pride
Nsp3 Sars-cov-2 Coronavirus Interactome Caco-2 Lc-ms/ms 
ProteomicsAn Integrative multi-omics landscape of SARS-CoVs NSP3
Proteomic analysis of SARS-CoV NSP3 expressing cells
ORGANISM(S): Homo Sapiens 
2021-11-03 | PXD023927 |
ProteomicsGlobal ISGylome analysis to identify substrates of SARS-CoV-2 Nsp3-PLpro
Interferon-stimulated gene 15 (ISG15) regulates diverse cellular processes, including antiviral immunity, through its conjugation to proteins (ISGylation). Several viruses, including SARS-CoV-2, reverse the process of protein ISGylation by encoding specialized enzymes harboring deISGylating enzymati...
ORGANISM(S): Homo sapiens (Human) 
2026-05-05 | PXD068808 | Pride
Sars-cov-2 Plpro Isg15 Deisgylation Isgylome Lc-ms/ms 
ProteomicsSINV-nsP3 mScarelt-tagged immunoprecipitation
1x107 cells HEK293 FLP-IN T-REX cells were infected with SINVnsP3-mScarlet (bait) or SINVwt (negative control) at 1 MOI. After 18 hours, cells were scrapped and spin down at 300 g 4°C for 10 mins. After the centrifugation, supernatants were discarded and cell pellet washed once with 1X PBS, followed...
ORGANISM(S): Homo sapiens (Human) 
2024-12-19 | PXD054117 | Pride
Sinv-nsp3 
ProteomicsThe NSP3 protein of SARS-CoV-2 binds fragile X mental retardation proteins to disrupt UBAP2L binding
Viruses interact with numerous host factors to facilitate viral replication and to dampen antiviral defense mechanisms. We currently have a limited mechanistic understanding of how SARS-CoV-2 binds host factors and the functional role of these interactions. Here, we uncover a novel interaction betwe...
ORGANISM(S): Homo sapiens (Human) 
2023-12-28 | PXD047232 | Pride
Sars-cov-2 Ubap2l Fragile x syndrome Stress granules Nsp3 
ProteomicsAPEX2-SFV nsP3 proteome identification upon APEX2-rSFV infection
Identifiy SFV nsP3 interactome via APEX2 proximity labeling in APEX2-rSFV infected U2OS cells
ORGANISM(S): Homo Sapiens 
2025-03-10 | PXD061676 |
ProteomicsInteracting Partners of CHIKV nsP3 in Human Macrophages
Chikungunya virus (CHIKV), an alphavirus transmitted by mosquitoes, has instigated several epidemics in recent years, sparking intensive efforts to understand its biology. Despite progress, the understanding of CHIKV’s molecular interactions with host cell constituents, especially in susceptible cel...
ORGANISM(S): Homo sapiens (Human) 
2024-03-06 | PXD045252 | Pride
Virus host interaction Macrophages Nonstructural protein 3 Proteome Chikv 
ProteomicsComparative Interactome Profiling of Nonstructural Protein 3 Across SARS-CoV-2 Variants Emerged During the COVID-19 Pandemic
SARS-CoV-2 virus and its variants have proven to be a threat to global health, in part due to their capacity for rapid evolution. SARS-CoV-2 variants that emerged throughout the COVID-19 pandemic exhibited variations in virulence, leading to diminished vaccine protection and modulating disease sever...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD061195 | Pride
Virus-host interactions Affinity purification-mass spectrometry Variants Nsp3 
Sort   by:  
 Page size