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ProteomicsBenchmarking multiple fragmentation methods on an Orbitrap Fusion for top-down phospho-proteoform characterization
Top-down analysis of intact proteins by mass spectrometry provides an ideal platform for comprehensive proteoform characterization, in particular, for the identification and localization of post-translational modifications (PTM) co-occurring on a protein. One of the main bottlenecks in top-down prot...
ORGANISM(S): Homo sapiens (Human) 
2015-07-16 | PXD001845 | Pride
Fragmentation Bora Ethcd Top down proteomics Phosphorylation Proteoform Orbitrap fusion 
ProteomicsC4PR_LIV Evaluation of Parameters for Confident Phosphorylation Site Localization using an Orbitrap Fusion Tribrid Mass Spectrometer
Confident identification of sites of protein phosphorylation by mass spectrometry (MS) is essential to advance understanding of phosphorylation-mediated signaling events. However, development of novel instrumentation requires that methods for MS data acquisition and its interrogation be evaluated an...
ORGANISM(S): Homo sapiens (Human) 
2019-09-27 | PXD007058 | Pride
Mascot/ptmrs Andromeda/ptmscore Orbitrap fusion Mass spectrometry Phosphoproteomics 
ProteomicsMirroring the charged termini; ETD/HCD fragmentation characteristics of LysargiNase and tryptic peptides and their benefits for peptide sequencing in proteomics
A key step in proteomics is the digestion of proteins into peptides, so far largely done by using trypsin. Tryptic digestion leads to peptides that in ESI-MS attain predominantly two charges, via protonation at the free N-terminus and at the C-terminal basic residue Arginine or Lysine. These peptide...
ORGANISM(S): Homo sapiens (Human) 
2016-12-02 | PXD004447 | Pride
Fragmentation Etd Hcd Lysarginase Orbitrap Phosphoproteomics Trypsin 
ProteomicsC4PR_LIV Evaluation of Parameters for Confident Phosphorylation Site Localization using an Orbitrap Fusion Tribrid Mass Spectrometer
Confident identification of sites of protein phosphorylation by mass spectrometry (MS) is essential to advance understanding of phosphorylation-mediated signaling events. However, development of novel instrumentation requires that methods for MS data acquisition and its interrogation be evaluated an...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-03-31 | MSV000085203 | MassIVE
ProteomicsAn optimised fragmentation regime for cross-linking/mass spectrometry
In this study we compared three different fragmentation techniques and two combined fragmentation schemes available on a novel tribrid mass spectrometer (Orbitrap Fusion Lumos, Themro Fisher Scientific) CID, HCD, ETD, ETD with supplemental CID (ETciD) and ETD with supplemental HCD (EThcD) on cross-l...
ORGANISM(S): Homo sapiens (Human) 
2016-08-02 | PXD003737 | Pride
Etcid Cid Cross-linking Ethcd Hcd Etd 
ProteomicsMapping physiological ADP-ribosylation using Activated Ion Electron Transfer Dissociation (AI-ETD)
ADP-ribosylation (ADPr) is a post-translational modification that plays pivotal roles in a wide range of cellular processes. Mass spectrometry (MS)-based analysis of ADPr under physiological conditions, without relying on genetic or chemical perturbation, has been hindered by technical limitations. ...
ORGANISM(S): Homo sapiens (Human) 
2020-09-23 | PXD017417 | Pride
Activated ion electron transfer dissociation Post-translational modification Adpr Ptm Parp Ai-etd Adp-ribosylation 
ProteomicsTop-Down Characterization of an Intact Monoclonal Antibody using Activated Ion-Electron Transfer Dissociation
Here, we examined the utility of AI-ETD for sequencing of the intact NIST monoclonal antibody on a modified Fusion Lumos Orbitrap platform using direct infusion. We also tested other dissociation techniques including HCD, ETD, and EThcD.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-10-19 | MSV000086331 | MassIVE
ProteomicsUnique Histone Proteoforms by Differential Top Down MS
H3.1 and H3.3 quadrupole isolated and fragmented by ETD and EThcD on an Orbitrap Fusion Lumos. Comparison of two cell lines.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-07-14 | MSV000079191 | MassIVE
ProteomicsThe effect of exercise on heart proteome
Mice were divided in two groups, one group was sedentary while the other were trained by swimming for 5 days a week for 5 months. After 5 months animals were sacrificed and left ventricles isolated for proteomics profiling. Proteins were isolated, fractionated and digested. Peptide fractions were an...
ORGANISM(S): Mus musculus (Mouse) 
2021-08-23 | PXD026558 | Pride
Mouse Left ventricle Lc-ms/ms 
ProteomicsCapillary zone electrophoresis-tandem mass spectrometry with activated ion electron transfer dissociation for large-scale top-down proteomics
Capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) has been recognized as an efficient approach for top-down proteomics recently for its high-capacity separation and highly sensitive detection of proteoforms. However, the commonly used collision-based dissociation methods often ca...
ORGANISM(S): Escherichia coli 
2019-06-11 | PXD012247 | Pride
Top-down proteomics E. coli Cze-ms Ai-etd 
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