Sort   by:  
 Page size 
GenomicsORC precisely positions nucleosomes at origins of replication
The budding yeast genome is marked by 250-350 origins of DNA replication. These origins are bound by the origin recognition complex (ORC) throughout the cell cycle. ORC has known DNA binding sequence preferences which, though necessary for binding, are not sufficient to fully specify a genomic locus...
ORGANISM(S): Saccharomyces cerevisiae 
2010-03-19 | E-GEOD-16926 | biostudies-arrayexpress
TranscriptomicsGenome-wide EMSA (gEMSA) of ORC and yeast genomic DNA
Eukaryotic DNA replication origins are selected in G1-phase when the origin recognition complex (ORC) binds chromosomal DNA, triggering a series of molecular events that culminate in the initiation of DNA replication (a.k.a. origin firing) during S-phase. Each chromosome requires multiple origins fo...
ORGANISM(S): Saccharomyces cerevisiae 
2013-09-30 | E-GEOD-48440 | biostudies-arrayexpress
TranscriptomicsORC binding profile in Candida albicans
Map ORC binding sites to identify replication origins in C. albicans by using polyclonal ORC antibodies (gift from Stephen Bell Lab). Due to the unsynchronized nature of Candida cells, log-phase cultures were taken to perfoem ChIP-chip experiments to find the genome-wide ORC binding sites. ChIP-chi...
ORGANISM(S): Candida albicans 
2014-02-13 | E-GEOD-54923 | biostudies-arrayexpress
TranscriptomicsTAP-ORC2 and control ChIP experiments in Drosophila Kc167 cells
Expression data from Kc167 cells under normal conditions. Used to assess expression levels of genes with ORC bound at promoter. Control experiments in support of our previous modencode submssion GSE17282. Kc167 cells were harvested from log phase gorwth. RNA extraction and hybridization on Affymet...
ORGANISM(S): Drosophila melanogaster 
2010-06-20 | E-GEOD-18942 | biostudies-arrayexpress
TranscriptomicsKc167 ORC2 Localization
modENCODE_submission_674 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known r...
ORGANISM(S): Drosophila melanogaster 
2010-05-15 | E-GEOD-17282 | biostudies-arrayexpress
ProteomicsYeast ORC sumoylation status fine-tunes origin activity
Sumoylation is emerging as a post-translation modification important for chromosome duplication and stability. The origin recognition complex (ORC), which directs DNA replication initiation by loading the MCM replicative helicases onto origins, is sumoylated in both yeast and human cells. However, t...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD032977 | Pride
Orc2 Sumo Yeast 
TranscriptomicsKc167 ORC Localization in hydroxyurea (HU)
Determination of the genome-wide distribution of ORC by chromatin immunoprecipitation in the Drosophila Kc167 cell line at the beginning of S phase. Kc167 cells were arrested at the G1/S transition with hydroxyurea (HU). Goal was to test whether the ORC binding distribution remained the same betwe...
ORGANISM(S): Drosophila melanogaster 
2014-12-05 | E-GEOD-63914 | biostudies-arrayexpress
GenomicsNucleoporins facilitate ORC loading onto chromatin
Nucleoporins facilitate ORC loading onto chromatin
PRJNA821897 | ENA
ProteomicsdBRWD3 manipulates chromatin signature by ubiquitination to regulate ORC
Ubiquitination and epigenetic modification both play essential roles in regulating cell fates and development, but little is known how two pathways crosstalk. A known chromatin-binding protein BRWD3, Bromodomain repeats and WD40 repeats domain contain protein 3, has been shown involved both in ubiqu...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-10-24 | PXD039708 | Pride
Histone Wd40 Degradation E3 ubiquitin ligase Dbrwd3 Orc 
ProteomicsCross-linking MS of S. cerevisiae ORC bound to DNA and Cdc6
During DNA replication initiation, the Origin Recognition Complex (ORC) and Cdc6 co-associate on DNA to load replicative helicases onto origins of replication. We apply DSSO crosslinking mass spectrometry of reconstituted ORC-DNA-Cdc6 to identify interactions between different subunits and domains i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-28 | PXD031033 | Pride
Cleavable Cid Xlms Xlinkx Crosslinking ms Dsso Phosphorylation Ms2_ms3 Orc 
Sort   by:  
 Page size