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ProteomicsAnalysis of conformational changes induced by binding of allosteric nanobodies to a bacterial LRRK2 homologue by CX-MS
As part of an in-depth characterization of nanobodies directed against the Chlorobium tepidum protein CtRoco, a bacterial LRRK2 homologue, chemical crosslinking combined with mass spectrometry (CX-MS) was performed. Two allosteric nanobodies binding to two different epitopes in the LRR and the Roc d...
ORGANISM(S): Chlorobium tepidum 
2024-04-23 | PXD046634 | Pride
Lrrk2 Dsso Nanobodies Crosslinking Ctroco 
ProteomicsMapping of nanobody binding sites on human LRRK2 by CX-MS
As part of an in-depth characterization of nanobodies directed against the human Leucine-rich repeat kinase 2 (LRRK2), epitopes of the nanobodies bound to LRRK2 were mapped by chemical crosslinking combined with mass spectrometry using the CID-cleavable crosslinker disuccinimidyl sulfoxide (DSSO).
ORGANISM(S): Homo sapiens (Human) 
2022-02-27 | PXD030063 | Pride
Cl-ms Lrrk2 Dsso Nanobodies Cx-ms Crosslinking Cid-cleavable 
ProteomicsCX-MS of DSSO-cross-linked A2M - Structural investigations of human α2-macroglobulin elucidate its native conformation and inhibitory mechanism
Human A2M was cross-linked with DSSO in its native, methylamine-treated, and trypsin-cleaved conformations. The monomer, dimer, and tetramer SDS-PAGE gel bands of native A2M were analyzed by in-gel digest. Total protein digests of all three conformations were analyzed and compared.
ORGANISM(S): Homo sapiens (Human) 
2021-05-21 | PXD019101 | Pride
Human a2m dsso 
ProteomicsA2M-3K DSSO cross-linking to identify the bait region position
An A2M mutant, A2M-3K, with the bait region arginines replaced with lysines, was cross-linked with DSSO. Its monomeric gel band was analyzed by XL-MS to identify the bait region position within a subunit.
ORGANISM(S): Homo sapiens (Human) 
2021-05-21 | PXD019048 | Pride
Human a2m 
ProteomicsThe structures of R124H and R555W TGFBIp suggest a direct role of the mutated residues in the aggregation of TGFBIp in granular corneal dystrophy
Protein aggregation in the outermost layers of the cornea, leading to cloudy vision and in severe cases blindness, is linked to mutations in the extracellular matrix protein, transforming growth factor-β-induced protein (TGFBIp). Among the most frequent disease-causing mutations are R124H and R555W,...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD022976 | Pride
Tgfbip X‐ray crystallography Extracellular matrix protein Lattice corneal dystrophy Granular corneal dystrophy Protein cross‐linking Tgfbi Protein aggregation 
ProteomicsStructure and molecular assembly of a MuvB transcription factor complex with the nucleosome
The core cell cycle machinery genes are transcriptionally regulated by the MuvB family of protein complexes in a cell cycle specific manner. During cell cycle exit in quiescence or senescence, the DREAM complex, which is the repressive form of MuvB, directs transcriptional repression of cell cycle g...
ORGANISM(S): Homo sapiens (Human) 
2022-08-17 | PXD031421 | Pride
Muvb Crosslinking 
ProteomicsCross-linking MS of S. cerevisiae ORC bound to DNA and Cdc6
During DNA replication initiation, the Origin Recognition Complex (ORC) and Cdc6 co-associate on DNA to load replicative helicases onto origins of replication. We apply DSSO crosslinking mass spectrometry of reconstituted ORC-DNA-Cdc6 to identify interactions between different subunits and domains i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-28 | PXD031033 | Pride
Cleavable Cid Xlms Xlinkx Crosslinking ms Dsso Phosphorylation Ms2_ms3 Orc 
ProteomicsSelennoprotein S interacts with the replication and transcription complex of the SARS-CoV-2 by binding nsp7
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) replicates and evades detection by using endoplasmic reticulum (ER) membranes and their associated protein machineries. Among these hijacked human ER proteins is selenoprotein S, which usually takes part in the ER protein degradation p...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD038629 | Pride
Selenos Nsp12 Nsp8 Covid-19 Vimp 
ProteomicsCryo-electron microscopy unravels microtubule inner protein networks reinforcing mammalian sperm flagella
To find and fuse with the egg, mammalian sperm must complete an arduous voyage through the female reproductive tract. This odyssey is powered by the sperm tail, a specialized motile cilium. Mammalian sperm tails are reinforced at the molecular scale with sperm-specific microtubule inner proteins (sp...
ORGANISM(S): Bos taurus (Bovine) 
2023-06-20 | PXD035941 | Pride
Sperm Proteomics Crosslinking ms Tubulin Dsso 
ProteomicsIn-cell structures of a conserved supramolecular array at the mitochondria-cytoskeleton interface in mammalian sperm
Mitochondria-cytoskeleton interactions modulate cellular physiology by regulating mitochondrial transport, positioning, and immobilization. However, there is very little structural information defining mitochondria-cytoskeleton interfaces in any cell type. Here, we use cryo-focused ion beam milling-...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2021-10-29 | PXD025562 | Pride
Boar sperm Cryo-electron tomography Proteomics Mitochondria Crosslinking 
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