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MetabolomicsLiquid Chromatography-Mass Spectrometry Quantification of Phytochemicals in Withania somnifera Using Data-Dependent Acquisition, Multiple-Reaction-Monitoring, and Parallel-Reaction-Monitoring with an Inclusion List

Characterization of botanical extracts by mass spectrometry-based metabolomics analysis helps in determining the phytochemical composition that underlies their bioactivity and potential health benefits, while also supporting reproducibility of effects in clinical trials. The quantification of sev...

2024-07-10 | MTBLS10418 | MetaboLights
ProteomicsLarge-Scale Targeted Proteomics Using Internal Standard Triggered-Parallel Reaction Monitoring
Development, implementation, and evaluation of a new data acquisition scheme called internal standard triggered-parallel reaction monitoring (IS-PRM) to increase the scale of targeted quantitative experiments while retaining high detection and quantification performance. All the details about the da...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080832 | MassIVE
ProteomicsParallel Reaction Monitoring of 42 peptide pairs with 15N metabolic labeling
To examine the properties of 14N and 15N labeled peptides from 15 metabolic labeling samples, we set up targeted quantification using Parallel Reaction Monitoring (PRM) on a high resolution, high accuracy mass spectrometer. We first examined the co-elution of light and heavy peptide forms. Furthermo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-06-09 | PXD030112 | Pride
Arabidopsis Quantification 15n metabolic labeling Protein prospector Targeted quantification Parallel reaction monitoring (prm) 
ProteomicsLarge-Scale Targeted Proteomics Using Internal Standard Triggered-Parallel Reaction Monitoring
Development, implementation, and evaluation of a new data acquisition scheme called internal standard triggered-parallel reaction monitoring (IS-PRM) to increase the scale of targeted quantitative experiments while retaining high detection and quantification performance. All the details about the da...
ORGANISM(S): Homo sapiens (Human) 
2016-02-09 | PXD001731 | Pride
Remote control Targeted proteomics Real-time data analysis Parallel reaction monitoring Quadrupole-orbitrap High resolution and accurate mass 
ProteomicsA Multiplexed Parallel Reaction Monitoring Assay to monitor bovine Pregnancy-Associated Glycoproteins throughout pregnancy and after gestation - PRM Data
Bovine pregnancy-associated glycoproteins (boPAGs) are extensively glycosylated secretory proteins of trophoblast cells. Roughly 20 different boPAG members are known but their distribution patterns and degree of glycosylation during pregnancy are not well characterized. The objective of the present ...
ORGANISM(S): Bos taurus (Bovine) 
2022-07-05 | PXD034108 | Pride
Parallel reaction monitoring Pregnancy Cattle 
ProteomicsParallel Reaction Monitoring to Assess High-Affinity N-Glycoforms of the Human Fcγ Receptor IIIa (CD16a)
Fc γ-receptor IIIa (FcγRIIIa, or CD16a) is a receptor for the Fc region of IgG antibodies and plays an essential role in the regulation of antibody effector functions. While the activation of CD16a plays a role in a healthy immune response, dysregulated CD16a activity may contribute to tissue damage...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD071784 | Pride
Glycosylation Cd16a Oligomannose Igg N162 Immunotherapy High mannose n-linked glycans Fcγriiia Parallel reaction monitoring Monocytes Fc γ (gamma) receptor iiia 
ProteomicsData-independent Acquisition - Parallel Reaction Monitoring (DIA-PRM) Acquisition Reveals Alteration of Lysosomal Hydrolases in a Mouse Model of Metachromatic Leukodystrophy
For the reproducible analysis of peptides by mass spectrometry-based proteomics, data-independent acquisition (DIA) and parallel/multiple reaction monitoring (PRM/MRM) deliver unrivalled performance with respect to sensitivity and reproducibility. Both approaches come, however, with distinct advanta...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-07 | PXD052313 | Pride
Dia Prm Targeted Mass spectrometry Mld Lysosomes 
ProteomicsDevelopment of a parallel reaction monitoring (PRM) mass spectrometry-assay for the detection of SARS-CoV-2 spike glycoprotein and nucleoprotein
There is an urgent need for robust and high-throughput methods for SARS-CoV-2 detection in suspected pa-tient samples to facilitate disease management, surveillance, and control. Although nucleic acid detection methods such as RT-PCR are the gold standard, during the current pandemic the deployment ...
ORGANISM(S): SARS coronavirus WHU 
2022-01-12 | PXD021080 | Pride
Skyline Nucleoprotein Qexactive hf-x Parallel reaction monitoring Sars-cov-2 Heavy standard peptides Spike protein 
ProteomicsApplying log-normal peak fitting to parallel reaction monitoring data analysis.
To support quantitative data analysis, we have developed a software, peakfit, that fits acquired chromatographic data to the log-normal peak equation and reports the calculated peak parameters. To demonstrate the capabilities of this approach, we provide hereby four example datasets: (1) 15 QCs of a...
ORGANISM(S): Homo sapiens (Human) 
2021-07-26 | PXD026875 | Pride
Data processing Peak modelling Peak fitting Parallel reaction monitoring Software tool 
ProteomicsPrecise diagnosis and typing of early-stage renal immunoglobulin-derived amyloidosis by label-free quantification of parallel reaction monitoring-based targeted proteomics
Untargeted proteomics based precise diagnosis and typing of amyloid deposits are crucial for guiding patient management. However, it may not be suitable for early-stage renal amyloidosis with minor damages. Here, we aimed to develop parallel reaction monitoring (PRM)-based targeted proteomics to ach...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD038265 | Pride
Laser microdissection Amyloidosis Kidney Parallel reaction monitoring Targeted proteomics 
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