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ProteomicsDietary protein in vitro digest (LC-MS)
16 dietary protein preparations were digested according to a standardized invitro digestion (InfoGest) protocol, simulating gastrointestinal digestion. Resulting oligopeptide mixtures were subsequently analyzed by UPLC-MS using DDA shot-gun detection. Peak intensity data from MS1 spectra were obtain...
ORGANISM(S): Bos taurus (Bovine) Pisum sativum (Garden pea) Solanum tuberosum (Potato) Zea mays (Maize) Triticum aestivum (Wheat) Saccharomyces cerevisiae (Baker's yeast) Gallus gallus (Chicken) 
2021-09-09 | PXD021577 | Pride
Dietary protein  in vitro digest  lc-ms  de novo 
ProteomicsAKT and AMPK site-specific phosphorylation on TBC1D4 biological activity
TBC1D4 is a 160 kDa multi-domain containing RabGAP (Rab GTPase-activating protein) and a downstream target of the insulin and contraction-activated kinases AKT and AMPK. While phosphorylation of TBC1D4 has been linked to GLUT4 translocation from storage vesicles (GSVs) to the cell surface, its impac...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-10 | PXD024645 | Pride
Lc-msms Partial digest Tbc1d4 
ProteomicsProtoemics on HIPPOCAMPi FROM PARTIAL-BODY IRRADIATED MICE
Background: The brain undergoes ionizing radiation exposure in many clinical situations, particularly during radiotherapy for brain tumors. The critical role of hippocampus in the pathogenesis of radiation-induced neurocognitive dysfunction is well recognized. Aim: The goal of this study is to test...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-10 | PXD024975 | Pride
2 weeks Shotgun proteomics 6 month Low radiation Hippocampus 
ProteomicsPhosphopeptide enrichment on synthetic libraries and HeLa cells digest
2 phosphopeptide enrichment methods (TiO2 and Ti-IMAC) were evaluated on synthetic libraries and HeLa cells digest. The resulting peptides were compared to the ones without enrichment with regards to the nature of the phosphorylation site (serine, threonine, tyrosine) and the characteristics of the ...
ORGANISM(S): Homo sapiens (Human) 
2014-08-11 | PXD000759 | Pride
Phosphopeptide enrichment Tio2 Ti-imac Hela Synthetic library 
GenomicsAlpha-galactosidase from Papiliotrema (Cryptococcus) flavescens
Partial sequence characterization of lactose-inducible long-chain alpha-galactosidase from Papiliotrema (Cryptococcus) flavescens
PRJEB20951 | ENA
ProteomicsEvaluation of different data-driven rescoring platforms to increase peptide identifications in HeLa digest samples
Evaluate the performance of different rescoring platforms with the aim of standardizing pipelines within our research group.
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD052875 | Pride
Hela samples Rescoring pipelines 
ProteomicsFLAG-PHF3 interactome in HEK293 cells
FLAG-hPHF3 was transiently expressed in human HEK293 cells, followed by anti-FLAG immunoprecipitation, on-bead digest and NanoLC-MS analysis.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026292 | Pride
Human Anti-flag immunoprecipitation Nanolc-ms analysis On-bead digest 
TranscriptomicsMesenchymal stromal cells isolated from bronchoalveolar lavage and digest parenchymal lung tissue
Stromal support is critical to the achievement of lung homeostasis and the maintenance of an effective epithelial barrier, and yet previous studies have found a positive association between the number of mesenchymal stromal cell populations (MSCs) isolated from the alveolar space and human diseases ...
ORGANISM(S): Homo sapiens 
2016-07-04 | E-GEOD-74284 | biostudies-arrayexpress
ProteomicsProteomics Using Proteases Alternative to Trypsin Benefits from Sequential Digestion with Trypsin
High specificity and ease of use make trypsin the most used enzyme in proteomics. Proteases with complementary cleavage specificity to trypsin have been applied to obtain additional data. However, use of proteases with broad specificity proved especially challenging. In this work, we analyzed the ch...
ORGANISM(S): Schizosaccharomyces pombe OY26 
2021-06-07 | PXD017321 | Pride
Alternative proteases Sequential digest 
ProteomicsSequential CRISPR screening reveals partial NatB inhibition as a strategy to mitigate alpha-synuclein levels in human neurons
Alpha-synuclein (αSyn) protein levels correlate with the risk and severity of Parkinson's disease and related neurodegenerative diseases. Lowering αSyn is being actively investigated as a therapeutic modality. Here we systematically map the regulatory network that controls endogenous αSyn using sequ...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD048283 | Pride
Crispr Alpha-synuclein Natb inhibition Parkinson's disease Human neurons 
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