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We combined glycopeptide enrichment by SPEG (Tian et al., 2007) with a hydrophobic segment-oriented hpTC method (Vít et al 2016) and a standard “detergent and trypsin” approach into a tree-pronged “Pitchfork” strategy for the analysis of membrane proteome in human lymphoma cells.
ORGANISM(S): Homo sapiens (Human) 
2019-03-29 | PXD012647 | Pride
In this study, we combined the transmembrane segment-oriented method hpTC with a solid phase enrichment of glycopeptides (SPEG) and standard 'detergent and trypsin' approach into a three-pronged 'Pitchfork' strategy to maximize membrane proteome coverage. The strategy enabled identification of over ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-05 | MSV000083404 | MassIVE
In order to identify new proteins with a potential to become targets for therapy or tumor imaging in pheochromocytoma and paraganglioma, we performed detailed proteomic analysis of 22 well-characterized human PPGL samples and normal chromaffin tissue from adrenal medulla. A standard quantitative pro...
ORGANISM(S): Homo sapiens (Human) 
2023-09-25 | PXD039788 | Pride
We combined glycopeptide enrichment by N-glyco-FASP (Zielinska et al., 2010), SPEG (Tian et al., 2007) with a hydrophobic segment-oriented hpTC method (Vít et al 2016) and a standard “detergent and trypsin” approach into a tree-pronged “Pitchfork” strategy for the analysis of membrane proteome in hi...
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD029344 | Pride
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